Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   J4N37_RS13130 Genome accession   NZ_CP071855
Coordinates   2769926..2771611 (+) Length   561 a.a.
NCBI ID   WP_010450185.1    Uniprot ID   A0A2K7SNG8
Organism   Vibrio sp. SCSIO 43153     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2764926..2776611
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J4N37_RS13110 (J4N37_12980) pdhR 2766537..2767304 (-) 768 WP_005446261.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  J4N37_RS13115 (J4N37_12985) ampD 2767709..2768260 (-) 552 WP_045393860.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  J4N37_RS13120 (J4N37_12990) nadC 2768353..2769240 (+) 888 WP_045393857.1 carboxylating nicotinate-nucleotide diphosphorylase -
  J4N37_RS13125 (J4N37_12995) - 2769468..2769926 (+) 459 WP_045393855.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  J4N37_RS13130 (J4N37_13000) pilB 2769926..2771611 (+) 1686 WP_010450185.1 type IV-A pilus assembly ATPase PilB Machinery gene
  J4N37_RS13135 (J4N37_13005) pilC 2771627..2772850 (+) 1224 WP_045393852.1 type II secretion system F family protein Machinery gene
  J4N37_RS13140 (J4N37_13010) pilD 2772921..2773790 (+) 870 WP_045393848.1 A24 family peptidase Machinery gene
  J4N37_RS13145 (J4N37_13015) coaE 2773791..2774405 (+) 615 WP_038885728.1 dephospho-CoA kinase -
  J4N37_RS13150 (J4N37_13020) zapD 2774434..2775174 (+) 741 WP_045393845.1 cell division protein ZapD -
  J4N37_RS13155 (J4N37_13025) yacG 2775231..2775431 (+) 201 WP_252041145.1 DNA gyrase inhibitor YacG -
  J4N37_RS13160 (J4N37_13030) rplS 2775802..2776155 (-) 354 WP_005379971.1 50S ribosomal protein L19 -

Sequence

Protein


Download         Length: 561 a.a.        Molecular weight: 62106.11 Da        Isoelectric Point: 4.9193

>NTDB_id=548416 J4N37_RS13130 WP_010450185.1 2769926..2771611(+) (pilB) [Vibrio sp. SCSIO 43153]
MLTNLCSVLRQAGLIDTQQEDAVIELVKASGISVPEALIHNMYFTSDELAVHLSSLFGLNYCSLCEFDFEPLCKELGLRE
LITKYNALPLKKDSNALTLAIPDPTMWHAEDDFRFATGLQVELVLADFKELQASIRRLYGRSIENSHSNLKDINQDELAN
LVDIGEDEIENIEDLSEDQSPVSRYINQILLDAVRKGASDIHFEPYENIFRVRLRCDGILVETQQPPSHLSRRLSARVKI
LSKLDIAERRLPQDGRIKLKLNSDVAIDMRVSTLPTLFGEKIVLRLLDSSSASLDIDKLGYSTEQKALYLNALKRPQGMI
LMTGPTGSGKTVSLYTGLNILNQPEVNISTAEDPVEINLSGINQVQVQPKIGFTFAEALRSFLRQDPDIVMVGEIRDLET
AEIAIKASQTGHLVLSTLHTNSAAETVVRLNNMGIASFNLASSLSLIIAQRLARRLCQNCKQPASMTPALSSLGISDKDH
VFDANPVGCSDCTNGYSGRTGIYEVMPFDDAMSEAIIKGYTSLELERLAVERGMKTLKASGLEKLKQGITSFSELQRVLY
L

Nucleotide


Download         Length: 1686 bp        

>NTDB_id=548416 J4N37_RS13130 WP_010450185.1 2769926..2771611(+) (pilB) [Vibrio sp. SCSIO 43153]
ATGCTCACCAACCTCTGCTCTGTTCTTCGTCAGGCTGGTTTAATTGATACTCAGCAAGAAGATGCCGTCATTGAGCTAGT
AAAAGCCTCTGGTATTTCGGTACCGGAGGCTTTGATCCATAACATGTATTTTACCTCAGATGAACTAGCGGTTCATTTGA
GCTCTTTGTTTGGCTTAAACTATTGCTCATTATGCGAGTTTGACTTTGAACCACTTTGTAAAGAGTTGGGGTTAAGAGAG
CTCATTACAAAATATAATGCTCTCCCTCTAAAAAAAGACTCCAATGCTCTTACGCTAGCAATTCCCGACCCCACAATGTG
GCATGCGGAAGATGATTTTAGGTTCGCTACAGGATTGCAAGTGGAGCTAGTCCTTGCAGACTTCAAAGAGCTTCAAGCTT
CTATACGTCGCTTATATGGCAGGTCAATTGAAAACTCTCACTCAAACTTAAAAGATATCAATCAAGACGAACTGGCTAAC
CTTGTAGATATTGGTGAAGACGAAATAGAAAACATCGAAGATTTAAGTGAGGATCAATCCCCAGTCAGCCGTTACATCAA
CCAAATTTTGCTCGACGCAGTCCGGAAAGGAGCTTCCGACATTCATTTTGAGCCATATGAAAACATCTTTCGTGTTCGGC
TACGTTGTGATGGCATCTTAGTTGAAACTCAACAGCCCCCTAGCCATCTTAGCCGTAGACTCTCTGCACGCGTAAAAATT
CTCTCTAAACTTGATATTGCAGAACGACGGTTACCACAAGATGGCCGAATTAAGCTCAAGTTAAATAGTGATGTCGCCAT
TGATATGCGCGTATCCACGCTCCCTACGTTATTTGGCGAAAAAATAGTATTACGCCTATTGGATAGCAGCTCCGCATCTT
TAGATATTGATAAGCTCGGATACAGTACAGAGCAGAAAGCTTTATATTTGAATGCCTTAAAACGCCCTCAAGGTATGATT
CTTATGACCGGCCCTACCGGTAGTGGAAAAACGGTGTCACTGTATACCGGGTTAAACATTCTAAACCAACCAGAAGTTAA
TATTTCTACAGCGGAAGATCCTGTTGAAATTAACTTATCTGGCATCAATCAAGTTCAAGTTCAACCAAAAATTGGTTTTA
CATTTGCAGAAGCACTGCGTTCATTTTTAAGGCAAGACCCTGATATTGTCATGGTTGGTGAAATACGAGATTTAGAAACT
GCCGAAATAGCAATAAAAGCCTCCCAAACTGGCCACCTCGTTCTATCGACACTGCATACAAATTCCGCTGCCGAAACGGT
TGTTCGCTTAAATAATATGGGCATTGCAAGCTTTAACCTAGCTTCCTCTCTAAGCCTTATTATTGCTCAACGTCTCGCGA
GACGTTTATGCCAAAACTGCAAGCAGCCAGCGTCAATGACTCCTGCGCTATCCTCACTGGGTATATCTGACAAAGACCAT
GTTTTTGACGCCAACCCTGTAGGGTGTAGTGATTGTACAAATGGTTACAGTGGGCGCACAGGGATTTATGAAGTAATGCC
ATTCGATGATGCAATGTCAGAGGCGATCATCAAAGGGTATACATCTCTGGAGCTTGAACGATTAGCTGTAGAGCGAGGAA
TGAAAACACTAAAGGCTTCAGGGTTAGAAAAACTAAAACAAGGGATCACCAGTTTTAGCGAGCTTCAACGCGTACTTTAC
TTATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(188-455)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2K7SNG8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio parahaemolyticus RIMD 2210633

77.005

100

0.77
  pilB Vibrio campbellii strain DS40M4

76.649

100

0.766
  pilB Vibrio cholerae strain A1552

70.745

100

0.711
  pilB Acinetobacter baylyi ADP1

50.269

99.287

0.499
  pilB Acinetobacter baumannii D1279779

51.692

94.831

0.49
  pilB Legionella pneumophila strain ERS1305867

48.463

98.574

0.478
  pilF Neisseria gonorrhoeae MS11

45.018

100

0.451
  pilF Thermus thermophilus HB27

37.367

100

0.374
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.097

99.465

0.369
  pilB/pilB1 Synechocystis sp. PCC 6803

37.004

98.752

0.365