Detailed information    

insolico Bioinformatically predicted

Overview

Name   recU   Type   Machinery gene
Locus tag   EXW53_RS07805 Genome accession   NZ_CP071808
Coordinates   1510018..1510620 (-) Length   200 a.a.
NCBI ID   WP_000155584.1    Uniprot ID   J8ATF7
Organism   Bacillus mycoides strain JAS014     
Function   plasmid transformation; homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1505018..1515620
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EXW53_RS07785 (EXW53_07805) dnaD 1505324..1506031 (+) 708 WP_000728545.1 DNA replication protein DnaD -
  EXW53_RS07790 (EXW53_07810) nth 1506047..1506694 (+) 648 WP_002201120.1 endonuclease III -
  EXW53_RS07795 (EXW53_07815) - 1506698..1507216 (+) 519 WP_002201119.1 YpoC family protein -
  EXW53_RS07800 (EXW53_07820) - 1507237..1509951 (-) 2715 WP_215558229.1 PBP1A family penicillin-binding protein -
  EXW53_RS07805 (EXW53_07825) recU 1510018..1510620 (-) 603 WP_000155584.1 Holliday junction resolvase RecU Machinery gene
  EXW53_RS07810 (EXW53_07830) - 1510724..1511749 (+) 1026 WP_002201117.1 DUF2515 domain-containing protein -
  EXW53_RS27765 - 1512045..1512191 (-) 147 WP_306475192.1 phage portal protein -
  EXW53_RS07815 (EXW53_07835) - 1512310..1512552 (+) 243 WP_002158558.1 hypothetical protein -
  EXW53_RS07820 (EXW53_07840) - 1512568..1512912 (+) 345 WP_002158556.1 YppE family protein -
  EXW53_RS07825 (EXW53_07845) - 1512938..1513129 (-) 192 WP_002030817.1 YppF family protein -
  EXW53_RS07830 (EXW53_07850) - 1513307..1513990 (+) 684 WP_215558230.1 YppG family protein -
  EXW53_RS07835 (EXW53_07855) - 1514147..1514551 (+) 405 WP_098988032.1 CotD family spore coat protein -
  EXW53_RS07840 (EXW53_07860) - 1514608..1515162 (+) 555 WP_002158553.1 DUF1273 domain-containing protein -
  EXW53_RS07845 (EXW53_07865) gpsB 1515260..1515595 (+) 336 WP_002201113.1 cell division regulator GpsB -

Sequence

Protein


Download         Length: 200 a.a.        Molecular weight: 23361.62 Da        Isoelectric Point: 9.5601

>NTDB_id=548009 EXW53_RS07805 WP_000155584.1 1510018..1510620(-) (recU) [Bacillus mycoides strain JAS014]
MTIRYPNGKRYDQASQPHKTPIKKHTYSNRGMSLEEELNETNQYYLTHNIACVHKKPTPLQIVKVDYPARSAAVVKEAYF
KQPSTTDYNGVYKGKYIDFEAKETKNKTSFPLQNFHLHQIEHMKQVVAHNGIAFVIIKFTLFDEFYLLDAKHIIAFWNRQ
NTGGRKSITKQEIEEHGSLLSCGYHPRIDYIRVLDTVYFS

Nucleotide


Download         Length: 603 bp        

>NTDB_id=548009 EXW53_RS07805 WP_000155584.1 1510018..1510620(-) (recU) [Bacillus mycoides strain JAS014]
ATGACCATTCGTTACCCAAATGGAAAAAGGTACGATCAAGCTTCACAACCTCATAAAACACCAATAAAAAAACATACTTA
CAGTAATAGAGGTATGTCCCTTGAAGAGGAATTGAATGAAACCAATCAATATTACTTAACCCATAATATTGCATGCGTAC
ATAAAAAACCTACACCTCTTCAAATTGTAAAAGTAGATTACCCCGCTCGAAGTGCTGCAGTGGTAAAAGAAGCGTATTTC
AAACAACCATCTACAACAGATTACAACGGTGTATACAAAGGGAAATATATCGATTTTGAAGCGAAAGAAACTAAAAATAA
AACCAGTTTTCCACTTCAAAACTTCCACCTTCATCAAATTGAACATATGAAGCAAGTAGTCGCTCATAATGGAATTGCAT
TTGTTATTATTAAATTTACACTTTTTGATGAATTTTATTTATTAGATGCAAAACATATTATTGCATTTTGGAATCGTCAA
AATACTGGTGGACGTAAATCGATTACAAAACAAGAAATAGAAGAGCATGGGTCTTTATTATCATGCGGTTATCACCCTCG
AATTGATTATATCCGCGTACTAGACACGGTTTATTTTTCGTGA

Domains

Predicted by InterproScan.

Recombination protein U (RecU)

(30-195)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB J8ATF7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recU Bacillus subtilis subsp. subtilis str. 168

63

100

0.63