Detailed information NTG0547298

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: J3S98_RS05660
• Genome accession: NZ_CP071729
• Coordinates: 1117511..1118092 (+)
• Length: 193 a.a.
• NCBI ID: WP_231097919.1
• Uniprot ID: -
• Organism: Lactococcus cremoris strain FM-YL11
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1112511..1123092

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
J3S98_RS05635 (J3S98_05635)	-	1112558..1113736 (+)	1179	WP_011676777.1	SLC13 family permease	-
J3S98_RS05640 (J3S98_05640)	-	1113909..1114469 (+)	561	WP_021165651.1	GNAT family N-acetyltransferase	-
J3S98_RS05645 (J3S98_05645)	-	1114586..1114984 (+)	399	WP_011676775.1	hypothetical protein	-
J3S98_RS05650 (J3S98_05650)	-	1115090..1116022 (+)	933	WP_014572202.1	ABC transporter ATP-binding protein	-
J3S98_RS05655 (J3S98_05655)	-	1116019..1117380 (+)	1362	WP_014572203.1	ABC transporter permease	-
J3S98_RS05660 (J3S98_05660)	comEA	1117511..1118092 (+)	582	WP_231097919.1	helix-hairpin-helix domain-containing protein	Machinery gene
J3S98_RS05665 (J3S98_05665)	comEC	1118220..1119491 (+)	1272	WP_021165650.1	ComEC/Rec2 family competence protein	Machinery gene
J3S98_RS12335	comEC	1119488..1119997 (+)	510	WP_021165649.1	MBL fold metallo-hydrolase	Machinery gene
J3S98_RS05670 (J3S98_05670)	-	1120074..1120964 (+)	891	WP_207873739.1	IS982 family transposase	-
J3S98_RS05675 (J3S98_05675)	-	1120985..1121290 (+)	306	Protein_1094	DNA internalization-related competence protein ComEC/Rec2	-
J3S98_RS05680 (J3S98_05680)	-	1121574..1122350 (+)	777	WP_021165645.1	alpha/beta hydrolase family protein	-
J3S98_RS05685 (J3S98_05685)	-	1122533..1122748 (+)	216	WP_014572212.1	F0F1 ATP synthase subunit C	-

Sequence

Protein

• Length: 193 a.a.
• Molecular weight: 21052.51 Da
• Isoelectric Point: 4.8079

>NTDB_id=547298 J3S98_RS05660 WP_231097919.1 1117511..1118092(+) (comEA) [Lactococcus cremoris strain FM-YL11]
MEFLYIFTNSPKQAENLSVENLSSSSTKSSVSKFNSSSEKNKNEIMVDLKGAVAKPNVYQISSDERLVDLIRQAGGFTDQ
ADQKSINLSAKLKDEEVIYVPKLGESSSSESTDSPISSSVSNQVSTTSGPKININKADLTELQKLTGIGQKKAQDIIDFR
MKNGDFKSIEDLGKVSGFGDKTLEKLKDEISID

Nucleotide

• Length: 582 bp

>NTDB_id=547298 J3S98_RS05660 WP_231097919.1 1117511..1118092(+) (comEA) [Lactococcus cremoris strain FM-YL11]
GTGGAATTTTTATATATTTTCACCAATTCACCAAAGCAAGCTGAAAATCTGTCAGTAGAAAATTTGAGCTCTAGTTCTAC
CAAAAGCTCTGTCAGTAAATTTAATAGCAGCAGTGAAAAAAATAAAAACGAAATTATGGTTGATTTGAAAGGAGCGGTTG
CAAAACCTAATGTATACCAAATTTCGTCAGATGAGCGCCTTGTTGATTTAATTAGGCAAGCGGGAGGATTTACTGACCAA
GCAGACCAAAAATCAATCAATCTGTCAGCGAAACTTAAAGATGAAGAAGTGATTTATGTACCAAAACTTGGGGAAAGTTC
AAGTTCAGAAAGTACTGACAGTCCTATTAGCAGCTCTGTCAGTAATCAAGTTTCAACGACAAGTGGTCCAAAAATAAATA
TCAATAAAGCAGACCTGACAGAATTACAAAAACTGACTGGAATTGGTCAAAAAAAAGCGCAAGATATTATTGATTTTCGC
ATGAAAAATGGTGACTTTAAATCAATAGAGGATTTGGGTAAAGTATCTGGCTTTGGGGATAAAACATTAGAAAAATTGAA
AGATGAGATTTCTATTGATTAA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (46-101)

• Helix-hairpin-helix motif (HHH_3) (129-190)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Lactococcus lactis subsp. cremoris KW2	94.792	99.482	0.943
comEA/celA/cilE	Streptococcus mitis SK321	45.213	97.409	0.44
comEA	Latilactobacillus sakei subsp. sakei 23K	42.781	96.891	0.415
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	48.993	77.202	0.378
comEA/celA/cilE	Streptococcus pneumoniae R6	48.322	77.202	0.373
comEA/celA/cilE	Streptococcus pneumoniae Rx1	48.322	77.202	0.373
comEA/celA/cilE	Streptococcus pneumoniae D39	48.322	77.202	0.373
comEA/celA/cilE	Streptococcus mitis NCTC 12261	47.651	77.202	0.368
comEA	Staphylococcus aureus MW2	40	90.674	0.363
comEA	Streptococcus thermophilus LMD-9	43.478	83.42	0.363