Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   XYFPCFBP8416_RS10175 Genome accession   NZ_CP087993
Coordinates   2238246..2238695 (+) Length   149 a.a.
NCBI ID   WP_012338101.1    Uniprot ID   -
Organism   Xylella fastidiosa subsp. multiplex strain CFBP8416     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2233246..2243695
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  XYFPCFBP8416_RS10155 (XYFPCFBP8416_010085) - 2233494..2234831 (+) 1338 WP_027700147.1 HAMP domain-containing sensor histidine kinase -
  XYFPCFBP8416_RS10160 (XYFPCFBP8416_010090) coaE 2235098..2235721 (-) 624 WP_004084593.1 dephospho-CoA kinase -
  XYFPCFBP8416_RS10165 (XYFPCFBP8416_010095) pilD 2235733..2236596 (-) 864 WP_004084594.1 A24 family peptidase Machinery gene
  XYFPCFBP8416_RS10170 (XYFPCFBP8416_010100) pilC 2236603..2237805 (-) 1203 WP_010894959.1 type II secretion system F family protein Machinery gene
  XYFPCFBP8416_RS10175 (XYFPCFBP8416_010105) pilA 2238246..2238695 (+) 450 WP_012338101.1 pilin Machinery gene
  XYFPCFBP8416_RS10180 (XYFPCFBP8416_010110) - 2238937..2239164 (-) 228 WP_012338102.1 hypothetical protein -
  XYFPCFBP8416_RS10185 (XYFPCFBP8416_010115) pilA/pilAI 2239997..2240437 (+) 441 WP_012338104.1 pilin Machinery gene
  XYFPCFBP8416_RS10190 (XYFPCFBP8416_010120) pilB 2241277..2243010 (+) 1734 WP_012338105.1 type IV-A pilus assembly ATPase PilB Machinery gene

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 15558.77 Da        Isoelectric Point: 8.4988

>NTDB_id=547048 XYFPCFBP8416_RS10175 WP_012338101.1 2238246..2238695(+) (pilA) [Xylella fastidiosa subsp. multiplex strain CFBP8416]
MKKQQGFNLIELMIVIAIIAVLAAIALPMYQNYVARSQLTAALADITPGKVQAESLIADGKSTSNASDIGLRTDTTRCGI
TVKIDAAGTANITCKVKGNSQVNDKTIVWDRTPDNSAGTNGVNNGGVWTCSSNVTSDALRPSGCMAAKQ

Nucleotide


Download         Length: 450 bp        

>NTDB_id=547048 XYFPCFBP8416_RS10175 WP_012338101.1 2238246..2238695(+) (pilA) [Xylella fastidiosa subsp. multiplex strain CFBP8416]
ATGAAAAAGCAACAAGGTTTTAACTTAATCGAACTAATGATCGTCATTGCAATCATTGCTGTTCTGGCGGCCATCGCTCT
GCCCATGTACCAAAATTATGTTGCCAGATCCCAACTCACTGCGGCACTTGCCGACATCACGCCGGGCAAAGTGCAAGCCG
AGTCTCTGATTGCTGATGGGAAGAGTACATCCAACGCGAGTGATATCGGTCTGCGTACCGACACCACTCGTTGTGGAATT
ACCGTTAAAATCGATGCCGCTGGCACTGCCAACATCACATGCAAAGTTAAGGGCAACTCTCAAGTCAATGATAAAACCAT
CGTTTGGGATCGTACCCCCGATAACTCTGCAGGCACCAATGGCGTCAATAATGGAGGTGTGTGGACCTGCTCCTCCAACG
TCACCTCCGATGCACTGAGACCTTCCGGCTGCATGGCTGCTAAACAATGA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)

Pilin (bacterial filament) (Pilin)

(35-144)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Pseudomonas aeruginosa PAK

38.994

100

0.416
  pilA/pilAI Pseudomonas stutzeri DSM 10701

42.177

98.658

0.416
  pilA/pilAII Pseudomonas stutzeri DSM 10701

42.177

98.658

0.416
  pilA Acinetobacter baumannii strain A118

40

100

0.403
  pilA Vibrio cholerae strain A1552

37.662

100

0.389
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

37.662

100

0.389
  pilA Vibrio cholerae C6706

37.662

100

0.389