Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   FH974_RS01920 Genome accession   NZ_CP071325
Coordinates   391338..393038 (-) Length   566 a.a.
NCBI ID   WP_047886942.1    Uniprot ID   A0A0J1H3H8
Organism   Photobacterium ganghwense strain C2.2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 386338..398038
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FH974_RS01890 (FH974_01890) mutT 386817..387215 (+) 399 WP_047886947.1 8-oxo-dGTP diphosphatase MutT -
  FH974_RS01895 (FH974_01895) yacG 387309..387527 (-) 219 WP_047887028.1 DNA gyrase inhibitor YacG -
  FH974_RS01900 (FH974_01900) zapD 387536..388273 (-) 738 WP_047886946.1 cell division protein ZapD -
  FH974_RS01905 (FH974_01905) coaE 388298..388912 (-) 615 WP_047886945.1 dephospho-CoA kinase -
  FH974_RS01910 (FH974_01910) pilD 388917..389786 (-) 870 WP_047886944.1 A24 family peptidase Machinery gene
  FH974_RS01915 (FH974_01915) pilC 390062..391282 (-) 1221 WP_047886943.1 type II secretion system F family protein Machinery gene
  FH974_RS01920 (FH974_01920) pilB 391338..393038 (-) 1701 WP_047886942.1 type IV-A pilus assembly ATPase PilB Machinery gene
  FH974_RS01925 (FH974_01925) pilA 393047..393469 (-) 423 WP_047886941.1 pilin Machinery gene
  FH974_RS01930 (FH974_01930) nadC 393940..394827 (-) 888 WP_047887027.1 carboxylating nicotinate-nucleotide diphosphorylase -
  FH974_RS01935 (FH974_01935) ampD 395127..395690 (+) 564 WP_047886939.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  FH974_RS01940 (FH974_01940) pdhR 395987..396760 (+) 774 WP_047886938.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62478.44 Da        Isoelectric Point: 5.3994

>NTDB_id=545048 FH974_RS01920 WP_047886942.1 391338..393038(-) (pilB) [Photobacterium ganghwense strain C2.2]
MQTNLASILHQADLLSLVEQQQVVDQVQSEGASVPSVLLQLGIIGSAELARQLEQLFALPLVDVHQYDYAECCKSLNLRD
LVLRHKVLPLYNTDTSIFIGLSDPTRIDVLDEFRFATGKNIEPLLLDHKQLEAAIRRVYGSDIKGAGSNKTQRSVSDADL
MELVDLTDTELADDATDLTSDNAPVTRYINQVLLDALRKSASDIHFEPYEESYRIRFRCDGILHLHASPPANLSRRLSTR
LKVMSRLNIAERRQPQDGRIKLKLSENVAVDLRVSTLPTLWGEKVVLRILDSSSANLDIDILGYTEPQKAAYLSALQRPQ
GMILMTGPTGSGKTVSLYTGLKILNTEERNISTAEDPVEINLPGINQVQIRPTVGLGFAEALRSFLRQDPDVVMVGEIRD
LETGSIAVKAAQTGHLVLSTLHTNSAAETITRLTNMGIENFNLASSLSLIIAQRLARRLCGHCKQPDDPDRFIRDTLAIP
DNATIFKASPTGCDECNKGYLGRVGIYEVMPFSRELAEALMAGANTLELEGIACRQSMKTLQQSGIEKLCEGITSLKELQ
RVLHFS

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=545048 FH974_RS01920 WP_047886942.1 391338..393038(-) (pilB) [Photobacterium ganghwense strain C2.2]
ATGCAAACAAACCTCGCCTCCATTCTTCACCAAGCAGATCTTCTCAGTCTGGTGGAGCAACAACAAGTGGTGGATCAGGT
TCAGTCAGAGGGCGCCTCGGTGCCCTCTGTTCTGTTGCAACTTGGCATCATCGGCAGCGCCGAGCTGGCCCGGCAACTGG
AACAGCTGTTCGCCCTGCCGCTGGTTGATGTCCATCAGTACGATTACGCGGAATGTTGCAAAAGCCTGAACCTGCGGGAT
CTGGTGTTACGCCACAAAGTGCTGCCGCTGTACAACACGGACACCAGTATTTTTATTGGCCTGAGCGATCCGACCCGTAT
TGATGTGCTGGATGAATTTCGCTTTGCCACCGGCAAAAACATTGAGCCGCTATTGCTGGATCATAAGCAGCTGGAAGCGG
CGATCCGCCGGGTATACGGCAGTGACATTAAAGGGGCCGGCTCCAACAAAACACAGCGCAGTGTCAGTGATGCAGATCTA
ATGGAGCTGGTGGATCTGACCGACACCGAACTGGCTGACGACGCCACAGATCTCACCAGTGATAACGCCCCGGTAACCCG
CTATATCAACCAGGTGCTACTGGACGCCCTGCGTAAAAGCGCTTCCGATATCCATTTTGAACCCTATGAAGAGAGCTATC
GGATCCGTTTTCGCTGTGACGGAATTTTGCATCTGCACGCCTCCCCACCGGCCAATTTGTCCCGTCGCTTATCCACCCGG
CTGAAGGTCATGTCCAGGCTCAATATTGCCGAGCGGCGCCAGCCTCAGGATGGCCGGATCAAGCTCAAATTGTCCGAAAA
TGTGGCCGTGGATTTGCGGGTTTCCACACTGCCTACCCTGTGGGGTGAAAAGGTGGTGCTGCGGATTCTTGACAGCTCCA
GTGCCAATCTGGATATCGATATTTTGGGTTATACCGAGCCCCAAAAAGCCGCATACCTGAGTGCCCTTCAGCGCCCTCAG
GGCATGATTCTGATGACCGGACCGACTGGCAGTGGCAAAACAGTCTCCCTGTACACCGGCCTCAAAATCCTGAACACCGA
GGAACGCAATATCTCCACCGCCGAAGACCCGGTGGAAATCAACCTGCCGGGCATAAATCAGGTACAGATACGCCCCACCG
TCGGGCTTGGGTTTGCCGAAGCACTGCGCTCATTCTTGCGCCAAGATCCGGATGTCGTCATGGTCGGGGAAATCAGGGAT
CTGGAAACCGGCTCTATTGCCGTGAAAGCAGCTCAGACTGGCCACCTGGTGTTGTCGACACTGCACACCAACTCAGCGGC
AGAAACCATTACCCGACTGACCAATATGGGGATCGAGAACTTCAACCTAGCATCGTCATTGAGCCTAATTATTGCTCAGC
GGCTGGCCCGCCGTCTTTGCGGACATTGCAAGCAGCCTGATGACCCGGATCGTTTTATCAGAGATACCCTCGCGATTCCC
GACAATGCCACCATTTTCAAAGCCAGCCCAACAGGGTGTGATGAGTGTAACAAAGGCTATCTGGGACGGGTCGGTATCTA
CGAGGTCATGCCCTTTTCACGAGAGCTCGCCGAAGCCTTAATGGCAGGTGCCAATACACTGGAGCTGGAAGGCATTGCCT
GTCGGCAAAGCATGAAAACCCTGCAACAGTCAGGTATAGAGAAGCTATGCGAAGGCATCACCAGTTTAAAAGAGTTACAA
CGCGTACTGCACTTTAGTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0J1H3H8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

62.301

99.823

0.622
  pilB Vibrio parahaemolyticus RIMD 2210633

60.531

99.823

0.604
  pilB Vibrio campbellii strain DS40M4

59.894

100

0.599
  pilB Acinetobacter baumannii D1279779

52.49

92.226

0.484
  pilB Acinetobacter baylyi ADP1

49.727

96.996

0.482
  pilB Legionella pneumophila strain ERS1305867

49.907

95.23

0.475
  pilF Neisseria gonorrhoeae MS11

43.21

100

0.433
  pilB/pilB1 Synechocystis sp. PCC 6803

37.437

100

0.392
  pilF Thermus thermophilus HB27

36.508

100

0.366