Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   J0J35_RS09260 Genome accession   NZ_CP071301
Coordinates   1877350..1878294 (+) Length   314 a.a.
NCBI ID   WP_165719887.1    Uniprot ID   -
Organism   Lactococcus sp. LG606     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1872350..1883294
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J0J35_RS09245 (J0J35_09245) - 1873611..1875212 (-) 1602 WP_206916722.1 GH25 family lysozyme -
  J0J35_RS09250 (J0J35_09250) guaB 1875381..1876862 (+) 1482 WP_003133714.1 IMP dehydrogenase -
  J0J35_RS09255 (J0J35_09255) rpsU 1876988..1877164 (+) 177 WP_003134539.1 30S ribosomal protein S21 -
  J0J35_RS09260 (J0J35_09260) comGA 1877350..1878294 (+) 945 WP_165719887.1 competence type IV pilus ATPase ComGA Machinery gene
  J0J35_RS09265 (J0J35_09265) comGB 1878233..1879251 (+) 1019 Protein_1790 competence type IV pilus assembly protein ComGB -
  J0J35_RS09270 (J0J35_09270) comYC 1879270..1879575 (+) 306 WP_029345656.1 competence type IV pilus major pilin ComGC Machinery gene
  J0J35_RS09275 (J0J35_09275) comGD 1879550..1879966 (+) 417 WP_206918196.1 competence type IV pilus minor pilin ComGD Machinery gene
  J0J35_RS09280 (J0J35_09280) comGE 1879998..1880231 (+) 234 WP_019293009.1 competence type IV pilus minor pilin ComGE -
  J0J35_RS09285 (J0J35_09285) comGF 1880218..1880652 (+) 435 WP_242534013.1 competence type IV pilus minor pilin ComGF -
  J0J35_RS09290 (J0J35_09290) - 1880884..1881729 (+) 846 WP_019291443.1 metal ABC transporter solute-binding protein, Zn/Mn family -
  J0J35_RS09295 (J0J35_09295) - 1881800..1882684 (-) 885 WP_086582775.1 RluA family pseudouridine synthase -

Sequence

Protein


Download         Length: 314 a.a.        Molecular weight: 35684.02 Da        Isoelectric Point: 6.9700

>NTDB_id=544812 J0J35_RS09260 WP_165719887.1 1877350..1878294(+) (comGA) [Lactococcus sp. LG606]
MIQVLAKNLLQKAVDQMVHDLYLVALEGKYSLYFRTTTERRFEGELTLEQGQALIAHMKFLSGMNLGESRRVQLGACTYI
LDKGEQRLRLSTVGDFHGQESLVIRLLHHQQNQLHFWNSEIFNTIIGGRGLYLFSGPVGSGKTSLMYKFAREHFKNQQVI
CIEDPVELVETEFLQLQVNKVIGNDYDALIKLSLRHRPDLLIVGEIRDSQTAKAVLRASLTGYTVFSTVHARSISGVIAR
LKELGLSDWELQSSLQRVIYQRLIAGKGLLVCEKEKFEEWKPDEWNDQIDQLVADGFISAVAAAREKIEFSKAD

Nucleotide


Download         Length: 945 bp        

>NTDB_id=544812 J0J35_RS09260 WP_165719887.1 1877350..1878294(+) (comGA) [Lactococcus sp. LG606]
ATGATACAGGTTTTGGCAAAAAACCTTTTACAAAAAGCAGTAGATCAGATGGTGCACGATCTTTATTTAGTAGCATTAGA
AGGAAAGTATTCTCTCTATTTTCGTACAACAACAGAAAGGAGGTTTGAAGGAGAACTCACCCTTGAGCAAGGGCAAGCAC
TGATTGCTCATATGAAATTTTTATCTGGTATGAATCTAGGAGAAAGTAGACGAGTACAGTTGGGGGCTTGCACTTATATC
TTAGATAAAGGTGAGCAGCGCTTACGTTTATCTACTGTCGGTGATTTTCATGGTCAAGAAAGCTTGGTTATTCGACTCTT
GCATCATCAGCAAAACCAACTTCATTTTTGGAATTCAGAGATCTTTAATACTATTATTGGGGGAAGAGGCCTTTACCTTT
TTTCAGGTCCTGTAGGTTCAGGTAAGACCAGTCTTATGTACAAATTTGCAAGGGAGCATTTTAAAAATCAACAAGTTATT
TGTATAGAAGATCCTGTGGAGTTAGTAGAAACTGAATTTTTACAGCTTCAAGTCAATAAAGTAATAGGGAATGATTATGA
TGCCTTGATTAAGCTTTCGCTGCGACACAGACCTGATTTACTTATTGTTGGTGAAATTAGGGATAGTCAAACGGCAAAAG
CTGTTCTTCGTGCAAGTTTGACTGGGTATACAGTATTCTCTACTGTTCATGCACGCTCAATATCAGGTGTAATTGCACGC
TTAAAAGAACTTGGATTAAGTGATTGGGAGCTACAATCCAGTCTTCAGCGGGTGATTTATCAACGATTAATTGCAGGAAA
GGGATTGCTTGTTTGTGAAAAGGAAAAGTTTGAAGAATGGAAACCAGACGAATGGAATGACCAGATTGATCAGTTGGTTG
CAGATGGATTTATCTCAGCTGTTGCAGCTGCGCGCGAAAAAATTGAGTTTAGCAAAGCAGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-264)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Lactococcus lactis subsp. cremoris KW2

60.191

100

0.602
  comYA Streptococcus gordonii str. Challis substr. CH1

53.333

100

0.535
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

53.035

99.682

0.529
  comGA/cglA/cilD Streptococcus pneumoniae R6

52.716

99.682

0.525
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

52.716

99.682

0.525
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

52.716

99.682

0.525
  comGA/cglA/cilD Streptococcus pneumoniae D39

52.716

99.682

0.525
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

52.564

99.363

0.522
  comYA Streptococcus mutans UA159

50.949

100

0.513
  comYA Streptococcus mutans UA140

50.949

100

0.513