Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   LPB19_RS15165 Genome accession   NZ_CP071247
Coordinates   3326255..3327970 (+) Length   571 a.a.
NCBI ID   WP_206643718.1    Uniprot ID   -
Organism   Marinobacter salinisoli strain LPB0319     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3321255..3332970
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LPB19_RS15150 (LPB19_15150) - 3322158..3323639 (-) 1482 WP_206643716.1 O-antigen ligase -
  LPB19_RS15155 (LPB19_15155) - 3323676..3325370 (-) 1695 WP_206643717.1 PglL family O-oligosaccharyltransferase -
  LPB19_RS15160 (LPB19_15160) pilA/pilAI 3325446..3325889 (-) 444 WP_323127892.1 pilin Machinery gene
  LPB19_RS15165 (LPB19_15165) pilB 3326255..3327970 (+) 1716 WP_206643718.1 type IV-A pilus assembly ATPase PilB Machinery gene
  LPB19_RS15170 (LPB19_15170) pilC 3327973..3329193 (+) 1221 WP_206643719.1 type II secretion system F family protein Machinery gene
  LPB19_RS15175 (LPB19_15175) pilD 3329264..3330139 (+) 876 WP_206643720.1 A24 family peptidase Machinery gene
  LPB19_RS15180 (LPB19_15180) coaE 3330143..3330742 (+) 600 WP_206643721.1 dephospho-CoA kinase -
  LPB19_RS15185 (LPB19_15185) - 3330714..3331832 (+) 1119 WP_206643722.1 hypothetical protein -
  LPB19_RS15190 (LPB19_15190) tsaA 3331835..3332578 (+) 744 WP_206643723.1 tRNA (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase TrmO -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 62628.44 Da        Isoelectric Point: 4.8049

>NTDB_id=544270 LPB19_RS15165 WP_206643718.1 3326255..3327970(+) (pilB) [Marinobacter salinisoli strain LPB0319]
MSNNKSSITLTGLARRFVDDGLLDEDTAKDAFLQASQNKIPLITYLAQNNLADSGKLASSAAQEFGFSVLDLDAFLPEMM
PEKAVDEKLIRKHSALPLYKRGNRLFVAVSDPTNIQALDEIKFNTGLSTDAVLVDDQKLRTAIDRYLESQDTSMGDLDDA
DLEGVETEAGDQDDQDTVSTAEVDDAPIVKYVNKMLLDAIRGGASDIHFEPYEKIYRVRYRTDGILKEVSRPSIKLAPKI
SARVKIMSQLDISERRVPQDGRIKMKLSKTKAIDFRVNTLPTLWGEKIVLRILDPSSAKLGIDALGYDEDQKALYLDALK
QPQGMILVTGPTGSGKTVSLYTGLNILNTPETNISTAEDPAEINLEGINQVNVNTKVGLGFAEALRAFLRQDPDVIMVGE
IRDLETANIAIKAAQTGHLVLSTLHTNSAPETLTRMMNMGVPAFNIATSVSLIIAQRLGRRLCNACKQPLDVPKDVLLQE
GFTEAQIETGFTLYRPKGCDKCSGGYKGRVGIYEVVKVTDELANMIMEEASSIQIAKQAQAEGFRNLRQSALIKVIEGVT
SLEEANRVTKD

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=544270 LPB19_RS15165 WP_206643718.1 3326255..3327970(+) (pilB) [Marinobacter salinisoli strain LPB0319]
ATGTCGAACAACAAAAGCAGCATCACACTCACCGGCCTGGCCAGGCGCTTTGTCGACGACGGCCTATTGGACGAAGACAC
TGCAAAAGACGCATTTCTCCAAGCATCTCAGAACAAAATCCCGCTGATCACCTACTTAGCACAAAACAATCTCGCCGATA
GCGGAAAGTTGGCATCCTCGGCGGCCCAGGAGTTTGGATTCTCTGTTCTGGATTTGGATGCTTTCTTGCCTGAGATGATG
CCCGAGAAAGCTGTTGACGAAAAACTGATCCGCAAACACAGCGCACTCCCCCTTTACAAACGAGGCAACCGCCTGTTTGT
CGCAGTATCTGACCCAACGAATATCCAAGCGCTGGACGAGATCAAGTTCAACACCGGCCTGAGCACCGACGCAGTTCTCG
TAGATGACCAGAAATTGCGCACGGCCATCGATAGGTACCTGGAGTCCCAGGACACCTCCATGGGCGACCTGGATGACGCA
GACCTCGAAGGCGTTGAAACGGAAGCCGGCGATCAGGATGATCAGGACACAGTATCCACAGCGGAAGTTGACGACGCACC
GATCGTCAAGTACGTCAACAAAATGCTGCTTGATGCAATTCGTGGCGGCGCATCAGACATCCACTTCGAGCCCTACGAAA
AGATCTATCGAGTCCGCTATCGGACCGACGGCATCCTGAAAGAGGTGTCGCGCCCCTCAATCAAGTTGGCGCCCAAGATT
TCCGCTCGAGTCAAGATCATGTCTCAACTGGACATCTCAGAGCGTAGAGTGCCGCAGGATGGCCGGATTAAGATGAAGCT
GTCCAAAACCAAGGCTATCGACTTCCGGGTAAACACTCTGCCCACCCTTTGGGGCGAAAAAATCGTATTGCGAATACTTG
ATCCCAGCTCCGCCAAACTGGGTATTGATGCACTGGGATATGACGAAGACCAAAAGGCACTTTACCTTGACGCCCTGAAG
CAGCCTCAAGGAATGATCTTGGTCACCGGCCCCACCGGCTCAGGCAAGACCGTGTCGCTATACACGGGCCTGAATATTCT
CAACACTCCGGAGACCAATATTTCGACTGCGGAGGATCCGGCTGAAATCAACCTTGAGGGCATCAACCAGGTCAACGTCA
ACACGAAGGTAGGACTGGGTTTTGCCGAAGCCCTGCGGGCCTTCCTGCGACAAGACCCTGACGTCATCATGGTGGGCGAG
ATCCGGGATCTTGAAACGGCCAATATCGCGATCAAGGCGGCACAAACCGGCCACCTGGTGCTGTCCACGCTTCACACTAA
CAGCGCACCAGAAACGCTTACTCGAATGATGAACATGGGCGTACCCGCGTTCAATATCGCCACCTCCGTAAGCCTGATCA
TCGCCCAACGCCTTGGCCGGCGCCTATGCAATGCCTGCAAACAACCCCTCGACGTACCTAAAGACGTGCTTCTTCAGGAA
GGGTTCACAGAGGCACAAATTGAAACAGGCTTCACGTTGTACCGACCCAAGGGCTGTGATAAATGCAGTGGGGGATACAA
AGGCCGAGTGGGTATCTACGAGGTTGTGAAGGTTACCGATGAACTGGCAAACATGATTATGGAAGAAGCCAGCTCCATTC
AAATTGCAAAACAGGCGCAGGCCGAAGGCTTCCGGAATTTGCGGCAATCCGCCCTTATTAAAGTGATTGAGGGCGTGACC
AGTCTCGAAGAAGCCAACCGCGTGACGAAGGATTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-152)

Type II/IV secretion system protein (T2SSE)

(194-461)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

58.217

100

0.583
  pilB Acinetobacter baylyi ADP1

56.818

100

0.569
  pilB Legionella pneumophila strain ERS1305867

56.007

99.124

0.555
  pilB Vibrio parahaemolyticus RIMD 2210633

52.244

97.548

0.51
  pilB Vibrio campbellii strain DS40M4

50.977

98.599

0.503
  pilB Vibrio cholerae strain A1552

55.556

89.842

0.499
  pilF Neisseria gonorrhoeae MS11

47.88

99.124

0.475
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.492

100

0.408
  pilB/pilB1 Synechocystis sp. PCC 6803

40.462

90.893

0.368
  pilF Thermus thermophilus HB27

38.868

92.82

0.361