Detailed information NTG0541058

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: JWR96_RS01995
• Genome accession: NZ_CP070856
• Coordinates: 393300..393956 (+)
• Length: 218 a.a.
• NCBI ID: WP_043735588.1
• Uniprot ID: A0AAX4AL53
• Organism: Lactococcus cremoris strain GR0507
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 388300..398956

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
JWR96_RS01970 (JWR96_01970)	-	388419..389597 (+)	1179	WP_021037692.1	SLC13 family permease	-
JWR96_RS01975 (JWR96_01975)	-	389771..390331 (+)	561	WP_011835699.1	GNAT family N-acetyltransferase	-
JWR96_RS01980 (JWR96_01980)	-	390448..390846 (+)	399	WP_043735595.1	hypothetical protein	-
JWR96_RS01985 (JWR96_01985)	-	390952..391884 (+)	933	WP_043735592.1	ABC transporter ATP-binding protein	-
JWR96_RS01990 (JWR96_01990)	-	391881..393242 (+)	1362	WP_043735590.1	ABC transporter permease	-
JWR96_RS01995 (JWR96_01995)	comEA	393300..393956 (+)	657	WP_043735588.1	helix-hairpin-helix domain-containing protein	Machinery gene
JWR96_RS02000 (JWR96_02000)	comEC	393937..396147 (+)	2211	WP_043735587.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
JWR96_RS02005 (JWR96_02005)	-	396428..397204 (+)	777	WP_014572211.1	alpha/beta hydrolase family protein	-
JWR96_RS02010 (JWR96_02010)	-	397387..397602 (+)	216	WP_004255250.1	F0F1 ATP synthase subunit C	-
JWR96_RS02015 (JWR96_02015)	atpB	397649..398362 (+)	714	WP_004255255.1	F0F1 ATP synthase subunit A	-
JWR96_RS02020 (JWR96_02020)	atpF	398377..398883 (+)	507	WP_010906128.1	F0F1 ATP synthase subunit B	-

Sequence

Protein

• Length: 218 a.a.
• Molecular weight: 23801.97 Da
• Isoelectric Point: 5.1730

>NTDB_id=541058 JWR96_RS01995 WP_043735588.1 393300..393956(+) (comEA) [Lactococcus cremoris strain GR0507]
MEKILDKFKEYWKIMLLVICALIAGGIFYIFTNSPKPAETLSVENLSSSSTKSSVSKFNSSSSEKNKNEIMVDLKGAVAK
PNVYQISSDERLVDLIRQAGGFTDQADQKSINLSAKLKDEEVIYVPKVGESSSSESTDSPTGSSVSNQISTTSGPKININ
KADLTELQKLTGIGQKKAQDIIDFRMKNGDFKSIEDLGKVSGFGDKTLEKLKDEISID

Nucleotide

• Length: 657 bp

>NTDB_id=541058 JWR96_RS01995 WP_043735588.1 393300..393956(+) (comEA) [Lactococcus cremoris strain GR0507]
ATGGAAAAAATTTTAGATAAATTCAAAGAATACTGGAAAATAATGCTTTTAGTTATTTGTGCACTTATTGCTGGTGGAAT
TTTTTATATTTTCACCAATTCACCAAAGCCAGCTGAAACTCTGTCAGTAGAAAATTTGAGCTCTAGTTCTACCAAAAGCT
CTGTCAGTAAATTTAATAGTAGCAGTAGTGAAAAAAATAAAAATGAAATTATGGTTGATTTAAAAGGAGCGGTTGCAAAA
CCTAATGTATACCAAATTTCGTCAGATGAGCGCCTTGTTGATTTAATTAGGCAAGCGGGAGGATTTACTGACCAAGCAGA
CCAAAAATCAATCAATCTGTCAGCGAAACTTAAAGATGAAGAAGTGATTTATGTACCCAAAGTTGGGGAAAGTTCAAGTT
CAGAAAGTACTGACAGTCCTACTGGTAGCTCTGTCAGTAATCAAATTTCAACGACAAGTGGTCCAAAAATAAATATCAAT
AAAGCAGACCTGACAGAATTACAAAAACTGACTGGAATTGGTCAAAAAAAAGCGCAAGATATTATTGATTTTCGCATGAA
AAATGGTGACTTTAAATCAATAGAGGATTTGGGTAAAGTATCTGGCTTTGGGGATAAAACATTAGAAAAACTGAAAGATG
AGATTTCTATTGATTAA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (71-126)

• Helix-hairpin-helix motif (HHH_3) (154-215)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Lactococcus lactis subsp. cremoris KW2	98.624	100	0.986
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	41.85	100	0.436
comEA/celA/cilE	Streptococcus mitis SK321	41.85	100	0.436
comEA/celA/cilE	Streptococcus pneumoniae R6	40.991	100	0.417
comEA/celA/cilE	Streptococcus pneumoniae Rx1	40.991	100	0.417
comEA/celA/cilE	Streptococcus pneumoniae D39	40.991	100	0.417
comEA/celA/cilE	Streptococcus mitis NCTC 12261	40.088	100	0.417
comEA	Latilactobacillus sakei subsp. sakei 23K	38.528	100	0.408
comEA	Staphylococcus aureus MW2	34.483	100	0.367
comEA	Staphylococcus aureus N315	34.498	100	0.362