Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   JWR96_RS00155 Genome accession   NZ_CP070856
Coordinates   39391..40416 (+) Length   341 a.a.
NCBI ID   WP_052206589.1    Uniprot ID   A0AAX4A9Z3
Organism   Lactococcus cremoris strain GR0507     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 34391..45416
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JWR96_RS00145 (JWR96_00145) - 34623..38396 (+) 3774 Protein_25 PolC-type DNA polymerase III -
  JWR96_RS00150 (JWR96_00150) comGA 38511..39491 (+) 981 WP_043736167.1 competence type IV pilus ATPase ComGA Machinery gene
  JWR96_RS00155 (JWR96_00155) comGB 39391..40416 (+) 1026 WP_052206589.1 competence type IV pilus assembly protein ComGB Machinery gene
  JWR96_RS00160 (JWR96_00160) comGC 40460..40759 (+) 300 WP_052206590.1 competence type IV pilus major pilin ComGC Machinery gene
  JWR96_RS00165 (JWR96_00165) comGD 40770..41201 (+) 432 WP_050750513.1 competence type IV pilus minor pilin ComGD Machinery gene
  JWR96_RS00170 (JWR96_00170) comGE 41173..41469 (+) 297 WP_043736169.1 competence type IV pilus minor pilin ComGE Machinery gene
  JWR96_RS00175 (JWR96_00175) comGF 41432..41878 (+) 447 WP_043736171.1 competence type IV pilus minor pilin ComGF Machinery gene
  JWR96_RS00180 (JWR96_00180) comGG 41902..42201 (+) 300 WP_021037947.1 competence type IV pilus minor pilin ComGG Machinery gene
  JWR96_RS00185 (JWR96_00185) - 42282..42719 (+) 438 WP_011677180.1 zinc-dependent MarR family transcriptional regulator -
  JWR96_RS00190 (JWR96_00190) - 42716..43558 (+) 843 WP_011677179.1 zinc ABC transporter substrate-binding protein -
  JWR96_RS00195 (JWR96_00195) - 43760..45132 (+) 1373 WP_185753013.1 IS3 family transposase -

Sequence

Protein


Download         Length: 341 a.a.        Molecular weight: 38471.23 Da        Isoelectric Point: 10.0626

>NTDB_id=541044 JWR96_RS00155 WP_052206589.1 39391..40416(+) (comGB) [Lactococcus cremoris strain GR0507]
MQTDILRLLRPKGKKLALIKQAKLIQLMGNLLNNGFHLGEVINFLALSKLVEKEFVFKMHQGLSSGNSLSEILESLSFSK
NVVTQLALVEVHGNLVGTMQLVELHLKKQLKVKKKLIEVATYPVVLFVFLIGIMWGLKNYLLPQIDKGNNFATLLINHLP
LVFFSVGALIVLLTSLSIIIFKHLSALTNFTFLVKIPLVSSFVRLYLTAYFSREWGNLIAQGVELRQIIELMKKQKSRIF
AEVGIKLDLGLKGGQNFEQAVSHFSIFLPELSLMIEYGAIKDKLGLELSVYADECWEQFFSKIDRLMQLIQPLIFIFVAL
MIVLLYAAMLLPIYSNMGSGI

Nucleotide


Download         Length: 1026 bp        

>NTDB_id=541044 JWR96_RS00155 WP_052206589.1 39391..40416(+) (comGB) [Lactococcus cremoris strain GR0507]
ATGCAAACGGACATCTTACGACTATTGAGGCCGAAAGGGAAAAAATTAGCATTAATCAAGCAAGCAAAATTGATACAACT
GATGGGGAATCTTTTGAATAATGGTTTCCATTTGGGAGAAGTTATCAATTTTTTAGCTTTATCAAAATTAGTAGAAAAAG
AGTTTGTATTCAAAATGCACCAAGGTCTCAGTTCTGGAAATTCATTATCTGAAATTTTAGAGAGTCTCTCTTTTTCTAAA
AATGTTGTGACACAACTTGCTTTGGTAGAAGTGCATGGGAATTTAGTAGGAACCATGCAATTGGTAGAACTGCATTTAAA
AAAGCAGCTTAAGGTCAAAAAGAAATTAATTGAAGTGGCAACTTATCCAGTTGTATTGTTCGTATTTCTCATTGGAATTA
TGTGGGGTTTGAAAAATTATTTATTACCTCAAATTGATAAGGGAAATAATTTTGCTACTTTATTAATTAATCATTTACCG
CTAGTATTTTTTTCTGTTGGTGCTTTAATCGTTTTACTAACAAGTTTATCAATAATAATATTCAAACATCTTTCAGCACT
CACTAATTTTACTTTTTTGGTCAAAATTCCATTAGTAAGTTCTTTTGTTCGCTTGTATTTGACCGCTTATTTCTCCAGAG
AATGGGGAAATTTAATTGCTCAAGGCGTTGAATTGCGACAAATAATAGAATTGATGAAAAAGCAGAAAAGTCGCATTTTT
GCGGAAGTTGGAATTAAACTTGATTTGGGTTTGAAGGGTGGTCAGAATTTTGAGCAAGCTGTCAGTCATTTTTCGATTTT
TTTACCAGAACTTTCTTTGATGATTGAGTACGGCGCGATTAAAGATAAATTAGGGTTGGAGCTTTCTGTTTATGCTGATG
AATGTTGGGAACAATTCTTTTCTAAGATAGACCGTCTAATGCAATTAATACAACCCCTTATTTTTATTTTTGTTGCTTTG
ATGATTGTCTTATTGTATGCAGCAATGTTGTTACCCATTTATTCAAATATGGGTTCTGGAATTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(211-332)

Type II secretion system (T2SS), protein F (T2SSF)

(25-143)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Lactococcus lactis subsp. cremoris KW2

97.654

100

0.977
  comYB Streptococcus gordonii str. Challis substr. CH1

51.929

98.827

0.513
  comGB/cglB Streptococcus mitis SK321

51.497

97.947

0.504
  comGB/cglB Streptococcus pneumoniae Rx1

50.898

97.947

0.499
  comGB/cglB Streptococcus pneumoniae D39

50.898

97.947

0.499
  comGB/cglB Streptococcus pneumoniae R6

50.898

97.947

0.499
  comGB/cglB Streptococcus pneumoniae TIGR4

50.898

97.947

0.499
  comGB/cglB Streptococcus mitis NCTC 12261

50.599

97.947

0.496
  comYB Streptococcus mutans UA140

51.592

92.082

0.475
  comYB Streptococcus mutans UA159

50.955

92.082

0.469