Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   JWR96_RS00175 Genome accession   NZ_CP070856
Coordinates   41432..41878 (+) Length   148 a.a.
NCBI ID   WP_043736171.1    Uniprot ID   A0AAX4AGE3
Organism   Lactococcus cremoris strain GR0507     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 36432..46878
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JWR96_RS00150 (JWR96_00150) comGA 38511..39491 (+) 981 WP_043736167.1 competence type IV pilus ATPase ComGA Machinery gene
  JWR96_RS00155 (JWR96_00155) comGB 39391..40416 (+) 1026 WP_052206589.1 competence type IV pilus assembly protein ComGB Machinery gene
  JWR96_RS00160 (JWR96_00160) comGC 40460..40759 (+) 300 WP_052206590.1 competence type IV pilus major pilin ComGC Machinery gene
  JWR96_RS00165 (JWR96_00165) comGD 40770..41201 (+) 432 WP_050750513.1 competence type IV pilus minor pilin ComGD Machinery gene
  JWR96_RS00170 (JWR96_00170) comGE 41173..41469 (+) 297 WP_043736169.1 competence type IV pilus minor pilin ComGE Machinery gene
  JWR96_RS00175 (JWR96_00175) comGF 41432..41878 (+) 447 WP_043736171.1 competence type IV pilus minor pilin ComGF Machinery gene
  JWR96_RS00180 (JWR96_00180) comGG 41902..42201 (+) 300 WP_021037947.1 competence type IV pilus minor pilin ComGG Machinery gene
  JWR96_RS00185 (JWR96_00185) - 42282..42719 (+) 438 WP_011677180.1 zinc-dependent MarR family transcriptional regulator -
  JWR96_RS00190 (JWR96_00190) - 42716..43558 (+) 843 WP_011677179.1 zinc ABC transporter substrate-binding protein -
  JWR96_RS00195 (JWR96_00195) - 43760..45132 (+) 1373 WP_185753013.1 IS3 family transposase -
  JWR96_RS00200 (JWR96_00200) - 45224..45961 (+) 738 WP_011836039.1 metal ABC transporter ATP-binding protein -
  JWR96_RS00205 (JWR96_00205) - 45954..46763 (+) 810 WP_011677177.1 metal ABC transporter permease -

Sequence

Protein


Download         Length: 148 a.a.        Molecular weight: 17074.81 Da        Isoelectric Point: 8.6779

>NTDB_id=541048 JWR96_RS00175 WP_043736171.1 41432..41878(+) (comGF) [Lactococcus cremoris strain GR0507]
MERKFCDLKLKIRAFTLLECLVTLLAISGSVLVISGLTRMIEEQMKISQNDSRKDWQIFCEQMRSELSGAKLDNVNQNFL
YVTKDKKLRFGLVGDDFRKSDDKGQGYQPMLYDLKGAKIQAEANLIKITIDFDNGGERVFIYRFTDTK

Nucleotide


Download         Length: 447 bp        

>NTDB_id=541048 JWR96_RS00175 WP_043736171.1 41432..41878(+) (comGF) [Lactococcus cremoris strain GR0507]
ATGGAAAGGAAATTTTGCGACTTGAAGCTCAAAATTAGGGCATTTACTTTACTAGAATGTCTCGTAACACTTTTGGCAAT
TTCTGGTTCAGTGCTAGTCATCTCAGGGCTGACTAGAATGATTGAAGAACAGATGAAAATAAGTCAGAATGATAGTAGGA
AAGATTGGCAAATATTTTGTGAACAAATGCGTTCAGAACTCTCCGGAGCAAAATTAGACAATGTGAATCAAAATTTTTTG
TATGTGACCAAAGATAAGAAGTTACGGTTCGGATTAGTAGGGGATGATTTTCGTAAAAGTGATGATAAAGGGCAAGGATA
CCAACCGATGCTTTATGATTTAAAAGGAGCAAAAATTCAGGCAGAAGCAAATTTGATAAAAATAACAATTGATTTTGATA
ATGGAGGTGAGCGAGTATTTATTTATCGATTTACAGATACAAAGTAA

Domains

Predicted by InterProScan.

Putative Competence protein ComGF (ComGF)

(45-137)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Lactococcus lactis subsp. cremoris KW2

97.872

95.27

0.932
  comYF Streptococcus mutans UA140

43.972

95.27

0.419
  comYF Streptococcus mutans UA159

43.262

95.27

0.412
  comGF/cglF Streptococcus mitis SK321

38.571

94.595

0.365
  comGF/cglF Streptococcus mitis NCTC 12261

38.571

94.595

0.365