Detailed information NTG0532761

Bioinformatically predicted

Overview

• Name: comGA
• Type: Machinery gene
• Locus tag: LLNCDO700_RS12105
• Genome accession: NZ_CP069179
• Coordinates: 2352030..2353010 (-)
• Length: 326 a.a.
• NCBI ID: WP_011836048.1
• Uniprot ID: A2RNS9
• Organism: Lactococcus cremoris strain NCDO700
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2347030..2358010

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LLNCDO700_RS12075 (LLNCDO700_12040)	-	2347104..2347463 (+)	360	WP_259692254.1	hypothetical protein	-
LLNCDO700_RS12080 (LLNCDO700_12045)	-	2347833..2348693 (+)	861	WP_259692253.1	S24 family peptidase	-
LLNCDO700_RS12085 (LLNCDO700_12050)	-	2348750..2349376 (+)	627	WP_259692252.1	OB-fold protein	-
LLNCDO700_RS12090 (LLNCDO700_12055)	-	2349499..2350956 (+)	1458	WP_259692251.1	recombinase family protein	-
LLNCDO700_RS12095 (LLNCDO700_12060)	comGC	2350953..2351087 (-)	135	WP_259683622.1	hypothetical protein	Machinery gene
LLNCDO700_RS12100 (LLNCDO700_12065)	comGB	2351105..2352130 (-)	1026	WP_042211510.1	competence type IV pilus assembly protein ComGB	Machinery gene
LLNCDO700_RS12105 (LLNCDO700_12070)	comGA	2352030..2353010 (-)	981	WP_011836048.1	competence type IV pilus ATPase ComGA	Machinery gene
LLNCDO700_RS12110 (LLNCDO700_12075)	-	2353124..2356906 (-)	3783	Protein_2363	PolC-type DNA polymerase III	-

Sequence

Protein

• Length: 326 a.a.
• Molecular weight: 36975.27 Da
• Isoelectric Point: 5.9451

>NTDB_id=532761 LLNCDO700_RS12105 WP_011836048.1 2352030..2353010(-) (comGA) [Lactococcus cremoris strain NCDO700]
MVQKKAQELIQKAIEMEASDIYLIASGNLYKIYIRQQLGRTLIEEVNQEIGLALLTHFKFLAGMNTGERRRVQLGACWYE
LEGSSAKRLRLSTVGDFEGNESLVIRLLHDQKQELEFWFDDKLQDFRCKRGLYLFAGPVGSGKTSLMFDLAHRHFSNAQV
ITIEEPVELIDSDFIQLQVNDVIGNSYDELIKLSLRHRPDLLIVGEIRDQQTARAVLRASLTGYTVFSTIHAASVKGVVQ
RLLELGLSDWELKNGLEAVVYQRLIAGKGVLDIAKSKFDTWSPKKWNEKIENLYANGHLTAIEAEREKISINQASKIDTT
DGESFE

Nucleotide

• Length: 981 bp

>NTDB_id=532761 LLNCDO700_RS12105 WP_011836048.1 2352030..2353010(-) (comGA) [Lactococcus cremoris strain NCDO700]
ATGGTACAGAAAAAAGCACAAGAACTCATTCAAAAGGCAATTGAGATGGAGGCTTCTGATATTTATTTAATTGCTTCAGG
AAATCTTTATAAGATATATATTCGTCAACAATTAGGGCGAACTTTAATAGAGGAAGTTAACCAAGAGATTGGTTTAGCAT
TACTTACTCATTTTAAATTTCTTGCTGGCATGAATACTGGTGAACGCCGGCGTGTTCAGCTGGGTGCTTGTTGGTATGAA
CTAGAGGGAAGTTCGGCAAAACGTTTGCGCCTTTCAACAGTGGGAGATTTTGAAGGAAACGAATCATTGGTTATCCGTCT
TCTGCATGACCAAAAACAAGAACTTGAGTTTTGGTTTGATGATAAACTTCAGGATTTTCGATGTAAACGAGGACTTTATT
TATTTGCTGGGCCAGTAGGGTCTGGGAAAACTTCACTAATGTTTGACTTAGCCCACCGTCATTTTTCAAATGCACAGGTT
ATCACTATTGAGGAGCCTGTAGAATTAATTGATTCTGATTTTATTCAGTTACAAGTTAATGATGTAATTGGGAATAGTTA
TGATGAATTAATTAAACTTTCTTTAAGACACCGACCAGATTTACTAATTGTTGGAGAAATTCGAGATCAGCAGACAGCTC
GTGCTGTATTGCGTGCAAGTTTAACAGGCTATACAGTTTTTTCTACTATTCATGCGGCTTCCGTGAAGGGAGTGGTTCAA
CGTTTGTTGGAATTGGGTTTGAGTGATTGGGAATTAAAAAATGGATTAGAGGCAGTTGTTTATCAACGGTTAATAGCGGG
AAAAGGAGTATTAGATATTGCGAAAAGTAAATTTGACACTTGGTCGCCGAAAAAATGGAATGAAAAGATTGAGAACTTAT
ATGCAAACGGACATCTTACGGCTATTGAGGCCGAAAGGGAAAAAATTAGCATTAATCAAGCAAGCAAAATTGATACAACT
GATGGGGAATCTTTTGAATAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (9-265)

3D structure

• AlphaFold DB: A2RNS9

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGA	Lactococcus lactis subsp. cremoris KW2	100	100	1
comGA/cglA	Streptococcus sobrinus strain NIDR 6715-7	56.129	95.092	0.534
comGA/cglA/cilD	Streptococcus pneumoniae D39	54.808	95.706	0.525
comGA/cglA/cilD	Streptococcus pneumoniae R6	54.808	95.706	0.525
comGA/cglA/cilD	Streptococcus pneumoniae TIGR4	54.808	95.706	0.525
comGA/cglA/cilD	Streptococcus pneumoniae Rx1	54.808	95.706	0.525
comGA/cglA/cilD	Streptococcus mitis NCTC 12261	54.808	95.706	0.525
comYA	Streptococcus gordonii str. Challis substr. CH1	54.114	96.933	0.525
comYA	Streptococcus mutans UA140	52.412	95.399	0.5
comYA	Streptococcus mutans UA159	52.412	95.399	0.5