Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   JNO49_RS03380 Genome accession   NZ_CP069162
Coordinates   752625..753749 (+) Length   374 a.a.
NCBI ID   WP_051229540.1    Uniprot ID   -
Organism   Paludibacterium yongneupense strain BCRC 80531     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 747625..758749
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JNO49_RS03365 - 747931..749361 (+) 1431 WP_028537172.1 deoxyribodipyrimidine photo-lyase -
  JNO49_RS03370 - 749764..750903 (+) 1140 WP_028537171.1 porin -
  JNO49_RS03375 - 751333..752484 (+) 1152 WP_028537170.1 porin -
  JNO49_RS03380 pilF 752625..753749 (+) 1125 WP_051229540.1 ATPase, T2SS/T4P/T4SS family Machinery gene
  JNO49_RS03385 - 753761..754948 (+) 1188 WP_028537169.1 type II secretion system F family protein -
  JNO49_RS03390 pilD 754949..755755 (+) 807 WP_028537168.1 A24 family peptidase Machinery gene
  JNO49_RS03395 coaE 755766..756395 (+) 630 WP_028537167.1 dephospho-CoA kinase -
  JNO49_RS03400 zapD 756427..757185 (+) 759 WP_028537166.1 cell division protein ZapD -
  JNO49_RS03405 yacG 757191..757379 (+) 189 WP_084637722.1 DNA gyrase inhibitor YacG -
  JNO49_RS03410 - 757383..758618 (-) 1236 WP_028537165.1 N-acetylmuramoyl-L-alanine amidase -

Sequence

Protein


Download         Length: 374 a.a.        Molecular weight: 39970.52 Da        Isoelectric Point: 8.7556

>NTDB_id=532568 JNO49_RS03380 WP_051229540.1 752625..753749(+) (pilF) [Paludibacterium yongneupense strain BCRC 80531]
MEAPAVRFLRQVVQQAVERQASDLHLEPLADGYRVRCRIDGVLHPLPAPAASLAVGVITRVKVLAGLDIAERRIPQDGRM
RLSRGDGQVLDVRVSTLPTLYGESVALRLQGTSSEMLDVSRLGLEPAQRDTLLAAIRRRSGLVLVTGPTGSGKTVTLYSL
LQVLNAPERKIATVEDPVEIPLPGVAQVAVNDKAGLSFAVALRAFLRQDPDVMMVGEIRDLETADIAIKAAHTGHLVLAT
LHTRDAPGSLARLLDMGVAPFSLVGTLSLVVAQRLLRRLCPHCRRREMPPSGVGGGTFIAVGCSLCHGTGYCGRVGVFEL
MPMSWNLGRLVMSGSAVDELAAQARREGMLDLADAAREKVRQGVTSQAEAMAAL

Nucleotide


Download         Length: 1125 bp        

>NTDB_id=532568 JNO49_RS03380 WP_051229540.1 752625..753749(+) (pilF) [Paludibacterium yongneupense strain BCRC 80531]
ATGGAAGCACCGGCTGTCCGTTTTTTGCGCCAGGTCGTCCAGCAGGCGGTGGAGCGGCAGGCGTCCGATCTGCATCTCGA
ACCCCTGGCCGACGGCTATCGCGTCCGCTGTCGTATCGACGGCGTGCTGCACCCGCTTCCGGCGCCCGCCGCATCGCTGG
CCGTGGGCGTCATCACCCGAGTGAAAGTGCTCGCCGGTCTCGACATCGCGGAAAGGCGGATACCGCAAGACGGGCGCATG
CGATTGAGCCGGGGCGACGGGCAAGTGCTGGACGTCAGGGTCAGCACCCTGCCGACCCTGTATGGCGAGAGCGTGGCCTT
GCGACTGCAAGGGACGAGCTCGGAAATGCTCGACGTGTCCCGGCTCGGGCTGGAACCGGCGCAACGCGATACGCTGCTGG
CGGCGATCCGCCGCCGCAGCGGCCTCGTGCTGGTGACCGGGCCGACCGGGAGCGGCAAGACCGTAACGCTCTACAGCCTG
CTGCAAGTGTTGAACGCACCGGAGCGAAAAATCGCGACGGTGGAAGATCCGGTCGAAATTCCGCTTCCCGGCGTGGCCCA
GGTTGCCGTCAACGACAAGGCGGGATTGAGCTTCGCCGTTGCCTTGCGCGCGTTTCTGCGCCAGGACCCCGATGTGATGA
TGGTCGGCGAGATCCGCGATCTGGAGACGGCGGATATCGCGATCAAGGCTGCGCATACCGGACACCTCGTCCTGGCGACC
CTGCATACGCGGGATGCACCGGGCAGCCTGGCCAGGTTGCTCGATATGGGGGTCGCTCCGTTCAGTCTGGTCGGGACGCT
GTCGCTGGTGGTCGCGCAACGCCTGTTGCGGCGGCTGTGTCCGCACTGCCGCCGCCGCGAGATGCCGCCCTCCGGCGTCG
GAGGCGGGACGTTCATCGCCGTGGGGTGCTCGCTCTGTCATGGAACCGGCTATTGCGGGCGGGTCGGTGTTTTCGAGTTG
ATGCCGATGTCCTGGAATCTGGGCCGGCTCGTCATGTCCGGTTCCGCGGTGGACGAACTGGCCGCCCAGGCCCGTCGGGA
AGGCATGCTCGATCTGGCGGACGCGGCGCGCGAAAAAGTCAGACAGGGTGTGACGTCGCAGGCGGAAGCCATGGCAGCAC
TGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-278)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

49.482

100

0.511
  pilB Vibrio campbellii strain DS40M4

49.602

100

0.5
  pilB Vibrio cholerae strain A1552

49.077

100

0.497
  pilB Legionella pneumophila strain ERS1305867

48.294

100

0.492
  pilB Vibrio parahaemolyticus RIMD 2210633

48.541

100

0.489
  pilB Acinetobacter baylyi ADP1

47.244

100

0.481
  pilB Acinetobacter baumannii D1279779

46.335

100

0.473
  pilB/pilB1 Synechocystis sp. PCC 6803

42.356

100

0.452
  pilF Thermus thermophilus HB27

43.603

100

0.447
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.708

100

0.438
  pilB Haemophilus influenzae 86-028NP

43.75

98.396

0.43
  pilB Haemophilus influenzae Rd KW20

43.207

98.396

0.425
  pilB Glaesserella parasuis strain SC1401

41.463

98.663

0.409
  ctsE Campylobacter jejuni subsp. jejuni 81-176

38.781

96.524

0.374