Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   LLF72_RS10575 Genome accession   NZ_CP068511
Coordinates   2076373..2077353 (-) Length   326 a.a.
NCBI ID   WP_015082934.1    Uniprot ID   A0AAJ6MJJ8
Organism   Lactococcus cremoris strain F.2.7     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2071373..2082353
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LLF72_RS10530 (LLF72_10325) - 2071391..2072128 (-) 738 WP_015082929.1 metal ABC transporter ATP-binding protein -
  LLF72_RS10535 (LLF72_10330) - 2072307..2073149 (-) 843 WP_011677179.1 metal ABC transporter solute-binding protein, Zn/Mn family -
  LLF72_RS10540 (LLF72_10335) - 2073146..2073583 (-) 438 WP_011677180.1 zinc-dependent MarR family transcriptional regulator -
  LLF72_RS10545 (LLF72_10340) comGG 2073663..2073962 (-) 300 WP_011677181.1 competence type IV pilus minor pilin ComGG Machinery gene
  LLF72_RS10550 (LLF72_10345) comGF 2073986..2074411 (-) 426 WP_373467301.1 competence type IV pilus minor pilin ComGF Machinery gene
  LLF72_RS10555 (LLF72_10350) comGE 2074395..2074631 (-) 237 WP_014573335.1 competence type IV pilus minor pilin ComGE Machinery gene
  LLF72_RS10560 (LLF72_13250) comGD 2074663..2074851 (-) 189 WP_014573336.1 hypothetical protein Machinery gene
  LLF72_RS10565 (LLF72_10360) comGC 2075053..2075403 (-) 351 WP_051013201.1 competence type IV pilus major pilin ComGC Machinery gene
  LLF72_RS10570 (LLF72_10365) comGB 2075448..2076473 (-) 1026 WP_051013189.1 competence type IV pilus assembly protein ComGB Machinery gene
  LLF72_RS10575 (LLF72_10370) comGA 2076373..2077353 (-) 981 WP_015082934.1 competence type IV pilus ATPase ComGA Machinery gene

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 37003.32 Da        Isoelectric Point: 5.9464

>NTDB_id=529207 LLF72_RS10575 WP_015082934.1 2076373..2077353(-) (comGA) [Lactococcus cremoris strain F.2.7]
MVQKKAQELIQKAIEMEASDIYLIASGNLYKIYIRQQLGRTLIEELNQEIGLALLTHFKFLAGMNTGERRRVQLGACWYE
LEGSSAKRLRLSTVGDFEGNESLVIRLLHDQKQELEFWFDDKLQDFRCKRGLYLFAGPVGSGKTSLMFDLAHRHFSNAQV
ITIEEPVELIESDFIQLQVNDVIGNSYDELIKLSLRHRPDLLIVGEIRDQQTARAVLRASLTGYTVFSTIHAASVKGVVQ
RLLELGLSDWELKNGLEAVVYQRLIAGKGVLDIAKSKFDTWSPKKWNEKIENLYANGHLTAIEAEREKISINQASKIDTT
DGESFE

Nucleotide


Download         Length: 981 bp        

>NTDB_id=529207 LLF72_RS10575 WP_015082934.1 2076373..2077353(-) (comGA) [Lactococcus cremoris strain F.2.7]
ATGGTACAGAAAAAAGCACAAGAACTCATTCAAAAGGCAATTGAGATGGAGGCTTCTGATATTTATTTAATTGCTTCAGG
AAATCTTTATAAGATATATATTCGTCAACAATTAGGGCGAACTTTAATAGAGGAACTTAACCAAGAGATTGGTTTAGCAT
TACTTACTCATTTTAAATTTCTTGCTGGCATGAATACTGGTGAACGCCGGCGTGTTCAGCTGGGTGCTTGTTGGTATGAA
CTAGAGGGAAGTTCGGCAAAACGTTTGCGCCTTTCAACAGTGGGAGATTTTGAAGGAAATGAATCATTGGTTATCCGTCT
TTTGCATGACCAAAAACAAGAACTTGAGTTTTGGTTTGACGATAAACTTCAGGATTTTCGATGTAAACGAGGACTTTATT
TATTTGCTGGGCCAGTAGGGTCTGGGAAAACTTCACTAATGTTTGACTTAGCCCACCGTCATTTTTCAAATGCACAGGTT
ATCACTATTGAGGAGCCTGTAGAATTAATTGAATCTGATTTTATTCAGTTACAAGTTAATGATGTAATTGGGAATAGTTA
TGATGAATTAATTAAACTTTCTTTAAGACACCGACCAGATTTACTAATTGTTGGAGAAATTCGAGATCAGCAGACAGCTC
GTGCTGTATTGCGTGCAAGTTTAACAGGCTATACAGTTTTTTCTACTATCCATGCGGCTTCCGTGAAGGGAGTGGTTCAA
CGTTTGTTGGAATTGGGTTTGAGTGATTGGGAATTGAAAAATGGATTAGAGGCAGTTGTTTATCAACGGTTAATAGCGGG
AAAAGGAGTATTAGATATTGCGAAAAGTAAATTTGACACTTGGTCGCCGAAAAAATGGAATGAAAAGATTGAGAACTTAT
ATGCAAACGGACATCTTACGGCTATTGAGGCCGAAAGGGAAAAAATTAGCATTAATCAAGCAAGCAAAATTGATACAACT
GATGGGGAATCTTTTGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Lactococcus lactis subsp. cremoris KW2

99.387

100

0.994
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

56.129

95.092

0.534
  comGA/cglA/cilD Streptococcus pneumoniae D39

54.808

95.706

0.525
  comGA/cglA/cilD Streptococcus pneumoniae R6

54.808

95.706

0.525
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

54.808

95.706

0.525
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

54.808

95.706

0.525
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

54.808

95.706

0.525
  comYA Streptococcus gordonii str. Challis substr. CH1

54.114

96.933

0.525
  comYA Streptococcus mutans UA140

52.412

95.399

0.5
  comYA Streptococcus mutans UA159

52.412

95.399

0.5