Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   JGS08_RS04220 Genome accession   NZ_CP067022
Coordinates   939481..940698 (-) Length   405 a.a.
NCBI ID   WP_007251659.1    Uniprot ID   A0A8T8BXM8
Organism   Pseudomonas cannabina pv. alisalensis strain MAFF 301419     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 934481..945698
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JGS08_RS04195 (JGS08_04195) tnpB 935320..935679 (+) 360 WP_007247726.1 IS66 family insertion sequence element accessory protein TnpB -
  JGS08_RS04200 (JGS08_04200) tnpC 935696..937228 (+) 1533 WP_007247725.1 IS66 family transposase -
  JGS08_RS04205 (JGS08_04205) yacG 937781..937990 (-) 210 WP_007251656.1 DNA gyrase inhibitor YacG -
  JGS08_RS04210 (JGS08_04210) coaE 937987..938610 (-) 624 WP_007251657.1 dephospho-CoA kinase -
  JGS08_RS04215 (JGS08_04215) pilD 938607..939479 (-) 873 WP_007251658.1 A24 family peptidase Machinery gene
  JGS08_RS04220 (JGS08_04220) pilC 939481..940698 (-) 1218 WP_007251659.1 type II secretion system F family protein Machinery gene
  JGS08_RS04225 (JGS08_04225) pilB 940701..942395 (-) 1695 WP_007251660.1 type IV-A pilus assembly ATPase PilB Machinery gene
  JGS08_RS04230 (JGS08_04230) - 942621..943010 (+) 390 WP_007251661.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  JGS08_RS04235 (JGS08_04235) - 943175..944482 (+) 1308 WP_080393985.1 O-antigen ligase -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44199.17 Da        Isoelectric Point: 10.1002

>NTDB_id=524502 JGS08_RS04220 WP_007251659.1 939481..940698(-) (pilC) [Pseudomonas cannabina pv. alisalensis strain MAFF 301419]
MASKAVKVSIYTWEGVDKKGAKTGGELSGHNLALVKAQLRKQGINPTKVREKSVSIFGKGKKIKPLDIAFFSRQMATMMK
AGVPLLQSFDIISEGAENPNMRALVGSLKQEVSAGNSFATALRKKPEYFDDLFCNLVDAGEQAGALESLLDRVASYKEKT
EKLKAKIKKAMTYPTAVLIVAFIVSGILLIKVVPQFQSVFAGFNAELPAFTLMVIALSEVVQQWWLAIVVLFFVTFFMLK
RAYKRSQKFRDRLDRFALKIPIIGPLIFKSCVARYARTLATTFAAGVPLVEALDSVAGATGNVVFRNAVNQVKQDVSTGM
QLNFSMRSTGVFPSLAIQMTAIGEESGALDAMLDKVATYYEEEVDNMVDSLTSLMEPMIMAVLGVIVGGLVIAMYLPIFK
LGSVV

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=524502 JGS08_RS04220 WP_007251659.1 939481..940698(-) (pilC) [Pseudomonas cannabina pv. alisalensis strain MAFF 301419]
ATGGCCAGCAAGGCAGTCAAAGTCAGCATTTACACTTGGGAAGGTGTCGACAAGAAAGGCGCAAAAACCGGCGGAGAACT
GAGCGGCCATAATCTGGCACTTGTGAAGGCCCAACTGCGTAAGCAAGGCATCAATCCGACCAAGGTACGCGAAAAGTCAG
TTTCTATTTTTGGCAAGGGCAAGAAGATCAAGCCACTGGACATTGCGTTCTTTTCTCGTCAGATGGCGACGATGATGAAG
GCTGGCGTGCCCTTGCTACAGTCATTCGACATCATCAGTGAGGGCGCAGAGAATCCAAATATGCGTGCGCTGGTCGGCTC
ACTGAAACAGGAAGTTTCAGCCGGTAACAGCTTCGCAACGGCCTTACGGAAGAAGCCTGAATACTTCGATGATTTGTTTT
GCAATCTGGTGGATGCCGGTGAGCAAGCAGGCGCCCTTGAAAGCCTGCTCGACCGAGTAGCAAGCTATAAGGAAAAGACT
GAAAAGCTCAAAGCCAAAATCAAGAAAGCCATGACCTATCCGACTGCCGTATTGATTGTGGCTTTTATTGTCTCAGGCAT
TTTGCTGATTAAAGTAGTACCGCAGTTTCAATCAGTTTTTGCCGGATTTAACGCCGAACTACCTGCATTTACTCTGATGG
TTATTGCGCTTTCGGAGGTTGTCCAACAGTGGTGGCTGGCAATTGTCGTACTGTTTTTTGTGACTTTTTTCATGCTCAAG
AGAGCCTACAAACGATCTCAAAAATTTCGTGACCGACTTGATCGTTTTGCATTGAAAATCCCCATTATCGGACCGCTTAT
TTTCAAATCCTGCGTCGCGCGATACGCACGCACTCTCGCTACCACGTTTGCGGCGGGTGTTCCACTGGTTGAGGCGCTGG
ACTCGGTCGCCGGAGCGACTGGCAATGTGGTGTTCCGGAACGCAGTCAATCAAGTCAAACAGGATGTATCTACAGGCATG
CAGCTGAATTTCTCGATGCGCTCGACAGGCGTTTTTCCTAGCCTCGCTATACAGATGACCGCGATTGGGGAAGAGTCCGG
CGCACTTGACGCGATGCTTGACAAGGTAGCGACGTATTACGAAGAAGAAGTCGACAACATGGTTGATAGCCTGACCAGCC
TGATGGAACCGATGATCATGGCGGTATTGGGTGTCATCGTCGGTGGTCTGGTCATTGCGATGTATCTGCCAATTTTCAAA
CTTGGAAGCGTCGTCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

73.827

100

0.738
  pilC Acinetobacter baylyi ADP1

59.314

100

0.598
  pilC Acinetobacter baumannii D1279779

59.653

99.753

0.595
  pilC Legionella pneumophila strain ERS1305867

52.02

97.778

0.509
  pilG Neisseria gonorrhoeae MS11

42.432

99.506

0.422
  pilG Neisseria meningitidis 44/76-A

42.432

99.506

0.422
  pilC Vibrio campbellii strain DS40M4

41.379

100

0.415
  pilC Vibrio cholerae strain A1552

41.235

100

0.412
  pilC Thermus thermophilus HB27

38.155

99.012

0.378