Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   JGS08_RS04225 Genome accession   NZ_CP067022
Coordinates   940701..942395 (-) Length   564 a.a.
NCBI ID   WP_007251660.1    Uniprot ID   A0A8T8BWX7
Organism   Pseudomonas cannabina pv. alisalensis strain MAFF 301419     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 935701..947395
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JGS08_RS04205 (JGS08_04205) yacG 937781..937990 (-) 210 WP_007251656.1 DNA gyrase inhibitor YacG -
  JGS08_RS04210 (JGS08_04210) coaE 937987..938610 (-) 624 WP_007251657.1 dephospho-CoA kinase -
  JGS08_RS04215 (JGS08_04215) pilD 938607..939479 (-) 873 WP_007251658.1 A24 family peptidase Machinery gene
  JGS08_RS04220 (JGS08_04220) pilC 939481..940698 (-) 1218 WP_007251659.1 type II secretion system F family protein Machinery gene
  JGS08_RS04225 (JGS08_04225) pilB 940701..942395 (-) 1695 WP_007251660.1 type IV-A pilus assembly ATPase PilB Machinery gene
  JGS08_RS04230 (JGS08_04230) - 942621..943010 (+) 390 WP_007251661.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  JGS08_RS04235 (JGS08_04235) - 943175..944482 (+) 1308 WP_080393985.1 O-antigen ligase -
  JGS08_RS04240 (JGS08_04240) - 944655..945941 (+) 1287 WP_042913808.1 polysaccharide biosynthesis C-terminal domain-containing protein -
  JGS08_RS04245 (JGS08_04245) - 945963..946340 (-) 378 WP_007251664.1 helix-turn-helix domain-containing protein -
  JGS08_RS04250 (JGS08_04250) gstA 946495..947115 (+) 621 WP_007251665.1 glutathione transferase GstA -

Sequence

Protein


Download         Length: 564 a.a.        Molecular weight: 62538.93 Da        Isoelectric Point: 6.3321

>NTDB_id=524503 JGS08_RS04225 WP_007251660.1 940701..942395(-) (pilB) [Pseudomonas cannabina pv. alisalensis strain MAFF 301419]
MTDVVLTGLAKQLVLAELLTETVAQQAYQQARRDKISLVSYLVLNKLVKSLTLAEMASDQFGVPFMDLACLDRESQPKGL
VSEKLVRQHHALPLWRRGNKLFIAISDPTNHQAVTDIQFSTGLNTEAILVEDDKLTTAIDRFFDSDSGLGNLEDVDLGLD
IEPSSDKETSLATQNDADDAPVVRFVNKMLMDAIRLGSSDLHFEPYEKIFRVRLRTDGILHEVARPPIHLANRIAARLKV
MASLDISERRKPQDGRVKLRVSKTKAIDFRMNTLPTLWGEKIVMRILDPTSAQMGIDALGYEPEQKALYLEALKLPQGMI
LVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINLEGINQVNVNPRQGLDFSQALRAFLRQDPDVIMVGEIRDLET
AEIAIKASQTGHMVLSTLHTNSAAETLTRLHHMGVAAFNIATAINLIIAQRLARKLCSHCKKELDIPHETLIKEGFPEAK
IGTFKIYGPVGCEHCNGGYRGRVGIYEVVKKTAELERIIMEEGNSLEISRQMRKDGFNDLRTSGLSKAMQGITSLEEVNR
VTKD

Nucleotide


Download         Length: 1695 bp        

>NTDB_id=524503 JGS08_RS04225 WP_007251660.1 940701..942395(-) (pilB) [Pseudomonas cannabina pv. alisalensis strain MAFF 301419]
ATGACTGATGTTGTCCTCACTGGTTTGGCAAAACAACTGGTTCTGGCCGAGCTACTTACTGAAACTGTGGCGCAGCAAGC
GTATCAACAGGCGCGCCGTGACAAGATTTCGTTGGTTAGCTATCTGGTTCTGAACAAACTGGTCAAAAGCCTGACGCTGG
CAGAAATGGCTTCTGACCAATTTGGTGTGCCGTTCATGGACCTTGCCTGCCTGGACAGGGAGAGTCAGCCAAAGGGACTG
GTAAGTGAAAAGCTGGTCAGACAGCACCATGCCCTCCCCCTGTGGCGACGTGGCAATAAACTATTCATCGCGATTTCGGA
CCCTACCAACCACCAGGCCGTGACTGATATTCAATTCAGCACGGGCCTGAATACTGAAGCGATTCTCGTCGAAGACGACA
AGCTGACCACAGCAATTGATCGCTTTTTTGACAGTGATAGCGGTCTTGGCAATCTGGAAGATGTCGATCTGGGCCTTGAC
ATCGAGCCCTCCAGTGACAAGGAAACCTCGCTTGCTACACAAAATGATGCCGATGACGCCCCAGTGGTGCGATTCGTCAA
CAAAATGCTGATGGACGCAATCCGGTTAGGATCATCCGATCTGCACTTCGAGCCCTATGAGAAAATTTTTCGCGTGCGCT
TGCGTACAGACGGCATCCTGCATGAAGTCGCCAGACCGCCCATTCATCTGGCAAACCGTATAGCTGCGCGCTTGAAAGTC
ATGGCCAGCCTCGATATATCGGAACGGCGCAAACCGCAGGACGGTCGGGTCAAGCTGCGGGTGTCCAAAACAAAAGCCAT
CGACTTCCGTATGAACACCTTGCCCACGTTATGGGGCGAGAAGATCGTGATGCGGATCCTCGACCCGACCAGCGCCCAAA
TGGGCATTGATGCTCTGGGCTACGAGCCAGAACAAAAGGCACTGTACCTGGAAGCGCTGAAACTGCCGCAAGGCATGATT
CTTGTAACTGGCCCTACAGGTTCCGGCAAGACCGTTTCCTTATATACCGGCCTGAATATCCTCAACACCGTGGACATCAA
CATTTCCACAGCTGAAGACCCGGTGGAGATCAACCTGGAAGGCATCAATCAAGTCAACGTCAACCCGCGCCAGGGATTGG
ACTTTTCTCAGGCACTACGCGCATTTCTGCGTCAAGATCCCGACGTAATCATGGTCGGTGAGATTCGAGACCTGGAAACC
GCTGAAATTGCCATCAAGGCATCTCAGACGGGTCATATGGTGCTATCAACCCTGCACACCAACAGCGCTGCGGAAACATT
GACGCGCCTGCACCACATGGGCGTCGCAGCATTCAATATTGCAACTGCGATCAACCTGATTATTGCGCAGCGACTCGCAC
GCAAATTATGCAGCCACTGCAAGAAGGAACTCGATATCCCTCATGAGACACTGATCAAAGAAGGCTTTCCGGAGGCTAAA
ATCGGTACATTCAAGATCTATGGCCCCGTAGGCTGCGAGCATTGCAATGGCGGCTACAGAGGCCGGGTCGGCATCTACGA
GGTGGTCAAGAAAACAGCAGAACTGGAGCGCATCATCATGGAAGAGGGCAACTCGCTGGAAATATCCAGGCAGATGCGTA
AGGACGGCTTCAATGACCTGCGCACCTCAGGCTTGTCAAAAGCCATGCAGGGCATAACCAGCCTTGAAGAAGTCAATCGC
GTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-147)

Type II/IV secretion system protein (T2SSE)

(188-455)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

58.082

99.823

0.58
  pilB Acinetobacter baylyi ADP1

56.762

99.645

0.566
  pilB Legionella pneumophila strain ERS1305867

55.752

100

0.559
  pilB Vibrio cholerae strain A1552

51.246

99.645

0.511
  pilB Vibrio campbellii strain DS40M4

49.291

100

0.493
  pilB Vibrio parahaemolyticus RIMD 2210633

50.455

97.34

0.491
  pilF Neisseria gonorrhoeae MS11

48.663

99.468

0.484
  pilF Thermus thermophilus HB27

38.693

100

0.388