Detailed information    

insolico Bioinformatically predicted

Overview

Name   comP   Type   Machinery gene
Locus tag   JH280_RS16340 Genome accession   NZ_CP066922
Coordinates   3755239..3755655 (+) Length   138 a.a.
NCBI ID   WP_274347446.1    Uniprot ID   -
Organism   Xanthomonas campestris strain 5053     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 3752045..3770021 3755239..3755655 within 0


Gene organization within MGE regions


Location: 3752045..3770021
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JH280_RS16320 (JH280_16260) - 3752045..3752683 (-) 639 WP_076054160.1 methionine biosynthesis protein MetW -
  JH280_RS16325 (JH280_16265) - 3752676..3753722 (-) 1047 WP_081390738.1 glycosyltransferase family 2 protein -
  JH280_RS16330 (JH280_16270) - 3753719..3754513 (-) 795 WP_076057322.1 glycosyltransferase family A protein -
  JH280_RS16335 (JH280_16275) - 3754518..3754898 (-) 381 WP_228431841.1 SMR family transporter -
  JH280_RS16340 (JH280_16280) comP 3755239..3755655 (+) 417 WP_274347446.1 pilin Machinery gene
  JH280_RS16345 (JH280_16285) - 3755740..3757722 (+) 1983 WP_274347447.1 hypothetical protein -
  JH280_RS16350 (JH280_16290) pilC 3757911..3759113 (+) 1203 WP_274349048.1 type II secretion system F family protein Machinery gene
  JH280_RS16355 (JH280_16295) - 3759120..3759983 (+) 864 WP_012437600.1 A24 family peptidase -
  JH280_RS16360 (JH280_16300) coaE 3759997..3760620 (+) 624 WP_274347448.1 dephospho-CoA kinase -
  JH280_RS21805 - 3760852..3761069 (+) 218 Protein_3144 SymE family type I addiction module toxin -
  JH280_RS16365 (JH280_16305) - 3761169..3762503 (-) 1335 WP_227971331.1 HAMP domain-containing sensor histidine kinase -
  JH280_RS16370 (JH280_16310) - 3762496..3763173 (-) 678 WP_003490678.1 response regulator transcription factor -
  JH280_RS16375 (JH280_16315) rimK 3763823..3764698 (-) 876 WP_076054142.1 30S ribosomal protein S6--L-glutamate ligase -
  JH280_RS16380 (JH280_16320) glgX 3765192..3767321 (+) 2130 WP_274347449.1 glycogen debranching protein GlgX -
  JH280_RS16385 (JH280_16325) - 3767851..3768243 (-) 393 WP_011038224.1 H-NS family nucleoid-associated regulatory protein -
  JH280_RS16390 (JH280_16330) - 3768334..3768729 (-) 396 WP_011038225.1 hypothetical protein -
  JH280_RS16395 (JH280_16335) - 3768913..3769059 (-) 147 WP_076054137.1 hypothetical protein -

Sequence

Protein


Download         Length: 138 a.a.        Molecular weight: 14262.39 Da        Isoelectric Point: 8.4935

>NTDB_id=523208 JH280_RS16340 WP_274347446.1 3755239..3755655(+) (comP) [Xanthomonas campestris strain 5053]
MKKNMQQGFTLIELMIVIAIIAILAAIALPAYSDYTKKAKVSEVVLAASSARTTISEFAAANNGTCPTANILESQTSTYV
NGVSNAGCIITAVSRVTGAAGNIVLAGTPNSVNFQVDWTCGGTVDAKFRPGSCQGTKQ

Nucleotide


Download         Length: 417 bp        

>NTDB_id=523208 JH280_RS16340 WP_274347446.1 3755239..3755655(+) (comP) [Xanthomonas campestris strain 5053]
ATGAAAAAGAATATGCAGCAGGGCTTCACCCTGATCGAACTGATGATCGTGATCGCCATCATCGCCATCCTGGCCGCCAT
CGCGCTGCCGGCCTATTCCGACTACACCAAGAAGGCCAAGGTCTCGGAAGTCGTGTTGGCTGCTTCGTCGGCTCGTACCA
CTATTTCAGAATTTGCAGCCGCCAATAACGGCACCTGCCCAACCGCCAACATTCTGGAAAGCCAGACCTCGACGTACGTC
AATGGCGTAAGCAACGCTGGCTGCATCATTACTGCTGTCAGCCGAGTAACCGGCGCCGCGGGTAACATCGTTCTGGCTGG
CACACCTAACTCGGTCAACTTCCAGGTCGACTGGACCTGCGGCGGCACCGTTGATGCGAAGTTCCGTCCGGGCTCTTGCC
AGGGCACCAAGCAGTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(4-29)

Pilin (bacterial filament) (Pilin)

(38-134)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comP Acinetobacter baylyi ADP1

46.622

100

0.5
  pilA2 Legionella pneumophila str. Paris

44.776

97.101

0.435
  pilA2 Legionella pneumophila strain ERS1305867

44.03

97.101

0.428
  pilE Neisseria gonorrhoeae MS11

35.669

100

0.406
  pilE Neisseria gonorrhoeae strain FA1090

35.897

100

0.406
  pilA Haemophilus influenzae 86-028NP

40.146

99.275

0.399
  pilA/pilAI Pseudomonas stutzeri DSM 10701

37.931

100

0.399
  pilA Glaesserella parasuis strain SC1401

35.526

100

0.391
  pilA Acinetobacter baumannii strain A118

38.571

100

0.391