Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   MRBBS_RS04200 Genome accession   NC_018268
Coordinates   927334..928554 (-) Length   406 a.a.
NCBI ID   WP_014870178.1    Uniprot ID   M1FHD7
Organism   Marinobacter sp. BSs20148     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 922334..933554
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MRBBS_RS04170 (MRBBS_0874) - 922891..923451 (-) 561 WP_014870172.1 YfaZ family outer membrane protein -
  MRBBS_RS04175 (MRBBS_0875) yacG 923665..923862 (-) 198 WP_014870173.1 DNA gyrase inhibitor YacG -
  MRBBS_RS04180 (MRBBS_0876) tsaA 923936..924703 (-) 768 WP_014870174.1 tRNA (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase TrmO -
  MRBBS_RS04185 (MRBBS_0877) - 924709..925818 (-) 1110 WP_187290773.1 hypothetical protein -
  MRBBS_RS04190 (MRBBS_0878) coaE 925790..926398 (-) 609 WP_014870176.1 dephospho-CoA kinase -
  MRBBS_RS04195 (MRBBS_0879) pilD 926455..927330 (-) 876 WP_014870177.1 prepilin peptidase Machinery gene
  MRBBS_RS04200 (MRBBS_0880) pilC 927334..928554 (-) 1221 WP_014870178.1 type II secretion system F family protein Machinery gene
  MRBBS_RS04205 (MRBBS_0881) pilB 928557..930272 (-) 1716 WP_014870179.1 type IV-A pilus assembly ATPase PilB Machinery gene
  MRBBS_RS04210 (MRBBS_0882) - 930578..931045 (+) 468 WP_014870180.1 pilin -
  MRBBS_RS04215 (MRBBS_0883) - 931185..932621 (+) 1437 WP_014870181.1 sulfatase-like hydrolase/transferase -

Sequence

Protein


Download         Length: 406 a.a.        Molecular weight: 44400.31 Da        Isoelectric Point: 9.7275

>NTDB_id=52315 MRBBS_RS04200 WP_014870178.1 927334..928554(-) (pilC) [Marinobacter sp. BSs20148]
MAQKANKLESYVWEGKDRRGNKAKGELSGVSVALAKAQLRKQGIVPDKVKKKSKPLFGGSKKITPFDIAMLTRQLATMMK
AGVPLVQSFDIVADGLENKGLQELVMSVRNDIASGNSFAGSLRKHPRQFDDLYCNLVDSGEKAGALEKMLDRIATYMEKT
EILKKKVKKAMTYPIAVVVVAIVVTAILLVKVVPQFESLFNGFGAELPVFTQMVVRLSEWMQTWWFVVLLGIVGTIVLFK
ESKRRSQKFSDIVDKYVLKVPVMGEILDKSAVAKFGRVLSTTFAAGVPLVDALESVAGATGNAVYRDAIMRIRNDVSGGT
QLQASMRAQDVFPVMAVQLTAIGEESGNLDEMLEKVAEHYEAVVDDMVDNLTALMEPMIMSVLGVLVGGLIIAMYLPIFQ
MGQVVG

Nucleotide


Download         Length: 1221 bp        

>NTDB_id=52315 MRBBS_RS04200 WP_014870178.1 927334..928554(-) (pilC) [Marinobacter sp. BSs20148]
ATGGCCCAAAAAGCGAATAAACTAGAATCCTATGTATGGGAAGGCAAAGATCGCCGCGGCAACAAAGCCAAGGGTGAGCT
GTCTGGCGTAAGCGTGGCTTTGGCCAAAGCCCAGTTACGCAAGCAAGGCATAGTTCCAGACAAGGTGAAAAAGAAATCCA
AGCCGTTGTTCGGTGGCAGCAAAAAAATCACTCCCTTTGACATTGCCATGCTCACCCGACAACTGGCCACCATGATGAAA
GCCGGTGTACCCCTGGTGCAAAGCTTTGACATTGTGGCCGATGGATTGGAAAACAAAGGCCTACAGGAACTGGTGATGTC
GGTTCGGAACGACATTGCTTCTGGTAACAGCTTCGCCGGATCGTTGCGTAAACACCCCCGCCAATTTGACGACTTGTACT
GCAACCTGGTGGATTCCGGCGAAAAGGCCGGTGCGCTCGAGAAAATGCTCGACCGTATCGCCACTTATATGGAAAAAACA
GAAATTCTGAAGAAAAAAGTAAAAAAAGCAATGACTTACCCGATCGCCGTTGTGGTAGTTGCGATCGTGGTAACAGCCAT
ATTGCTGGTAAAAGTAGTTCCGCAATTTGAATCGCTTTTTAACGGGTTTGGTGCAGAGCTGCCAGTGTTTACCCAGATGG
TGGTTCGCCTGTCAGAATGGATGCAGACATGGTGGTTCGTAGTGCTACTAGGCATTGTCGGCACCATTGTTTTATTCAAA
GAGTCAAAACGGCGGTCACAGAAGTTTTCAGACATTGTCGACAAATATGTGCTGAAAGTGCCGGTTATGGGTGAAATTCT
GGACAAATCTGCCGTCGCGAAGTTTGGCCGGGTGTTATCAACAACGTTCGCAGCCGGTGTTCCCCTTGTAGATGCTCTTG
AATCCGTTGCGGGCGCTACCGGCAACGCGGTTTATCGCGATGCCATCATGCGCATTCGCAATGACGTTTCCGGCGGCACC
CAGCTTCAAGCCTCTATGCGGGCGCAGGACGTGTTTCCGGTGATGGCGGTTCAGCTGACGGCGATTGGCGAGGAATCTGG
TAATCTGGACGAAATGCTGGAAAAAGTGGCCGAGCACTACGAAGCCGTAGTGGATGACATGGTAGACAACCTGACTGCGC
TGATGGAGCCAATGATCATGTCGGTGTTGGGCGTGCTGGTGGGTGGTTTGATTATCGCCATGTACCTGCCGATCTTCCAA
ATGGGCCAGGTTGTTGGGTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(277-397)

Type II secretion system (T2SS), protein F (T2SSF)

(72-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB M1FHD7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

67.901

99.754

0.677
  pilC Acinetobacter baumannii D1279779

57.531

99.754

0.574
  pilC Acinetobacter baylyi ADP1

57.143

100

0.571
  pilC Legionella pneumophila strain ERS1305867

53.448

100

0.534
  pilG Neisseria gonorrhoeae MS11

43.21

99.754

0.431
  pilG Neisseria meningitidis 44/76-A

42.857

100

0.429
  pilC Vibrio campbellii strain DS40M4

40.049

100

0.401
  pilC Vibrio cholerae strain A1552

40

98.522

0.394

Multiple sequence alignment