Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   MRBBS_RS04205 Genome accession   NC_018268
Coordinates   928557..930272 (-) Length   571 a.a.
NCBI ID   WP_014870179.1    Uniprot ID   M1FCV5
Organism   Marinobacter sp. BSs20148     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 923557..935272
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MRBBS_RS04175 (MRBBS_0875) yacG 923665..923862 (-) 198 WP_014870173.1 DNA gyrase inhibitor YacG -
  MRBBS_RS04180 (MRBBS_0876) tsaA 923936..924703 (-) 768 WP_014870174.1 tRNA (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase TrmO -
  MRBBS_RS04185 (MRBBS_0877) - 924709..925818 (-) 1110 WP_187290773.1 hypothetical protein -
  MRBBS_RS04190 (MRBBS_0878) coaE 925790..926398 (-) 609 WP_014870176.1 dephospho-CoA kinase -
  MRBBS_RS04195 (MRBBS_0879) pilD 926455..927330 (-) 876 WP_014870177.1 prepilin peptidase Machinery gene
  MRBBS_RS04200 (MRBBS_0880) pilC 927334..928554 (-) 1221 WP_014870178.1 type II secretion system F family protein Machinery gene
  MRBBS_RS04205 (MRBBS_0881) pilB 928557..930272 (-) 1716 WP_014870179.1 type IV-A pilus assembly ATPase PilB Machinery gene
  MRBBS_RS04210 (MRBBS_0882) - 930578..931045 (+) 468 WP_014870180.1 pilin -
  MRBBS_RS04215 (MRBBS_0883) - 931185..932621 (+) 1437 WP_014870181.1 sulfatase-like hydrolase/transferase -
  MRBBS_RS04220 (MRBBS_0884) - 932889..934097 (+) 1209 WP_014870182.1 serine hydrolase domain-containing protein -
  MRBBS_RS04225 - 934231..934665 (+) 435 WP_041635469.1 pilin -
  MRBBS_RS04230 (MRBBS_0887) - 934946..935263 (+) 318 WP_014870185.1 antibiotic biosynthesis monooxygenase family protein -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 62317.45 Da        Isoelectric Point: 5.6148

>NTDB_id=52316 MRBBS_RS04205 WP_014870179.1 928557..930272(-) (pilB) [Marinobacter sp. BSs20148]
MTTNTSNITLTGLARRFVSDGLLDESAAKDAFFQASQNRIPLITYLTQHKLANSSALAFSAAMEFGISVLDLDAFLPEAM
PDKLVDEKLLRKHNALPLYKRGNRLFIAVSDPTNIQALDEIKFNTGLSTDAILVDDAKLRAAIEKHLASSESSMGDLGDT
DLEGIETEGADGNEDDGAVVVAEIDDAPIVKYVNKMLLDAIRGGSSDVHFEPYEKTYRVRFRTDGILKEMSRPSIKLAPK
ISARIKIMAQLDISERRLPQDGRIKMRLSKTKAIDFRVNTLPTLWGEKIVLRILDPSQAKLGIDALGYEEDQKKLYMDAL
AQPQGMILVTGPTGSGKTVSLYTGLNILNTDERNISTAEDPAEINLEGVNQVNVNNKVGLGFAEALRAFLRQDPDVIMVG
EIRDLETANIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIATSVSLIIAQRLGRRLCSCKQAGSIPKDVLLAE
GFSTEQIATGFTLYHPKGCDKCTNGYKGRVGIYEVVKVTEQLANMIMEEASSIKIAKAAQAEGFRTLRQSALLKVIEGLT
SLEEANRVTKD

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=52316 MRBBS_RS04205 WP_014870179.1 928557..930272(-) (pilB) [Marinobacter sp. BSs20148]
ATGACAACAAACACAAGCAACATCACCTTAACGGGTCTGGCACGGCGCTTTGTAAGCGACGGCCTGCTGGATGAATCCGC
GGCCAAAGACGCCTTTTTTCAGGCTTCTCAGAACCGTATTCCGCTGATCACCTACCTGACACAGCACAAGCTGGCAAACA
GCTCGGCGTTGGCGTTTTCGGCGGCTATGGAGTTTGGTATTTCAGTGCTGGACCTGGATGCGTTTTTGCCAGAGGCGATG
CCAGACAAGCTGGTGGACGAAAAACTGCTGCGCAAGCACAACGCCCTGCCTCTTTATAAGCGTGGCAACCGGCTGTTTAT
TGCGGTGAGCGACCCCACCAACATTCAGGCTCTGGACGAGATCAAGTTCAACACCGGCTTAAGTACCGATGCGATTCTGG
TGGACGACGCCAAGCTGCGCGCAGCTATCGAAAAGCACCTGGCGTCCAGTGAAAGCTCCATGGGCGACTTGGGTGATACA
GACCTTGAAGGTATAGAAACAGAAGGCGCGGATGGAAACGAGGATGACGGCGCCGTTGTAGTTGCAGAAATAGACGATGC
GCCCATCGTGAAGTATGTGAATAAGATGCTGCTGGATGCCATTCGCGGAGGCTCGTCAGACGTTCACTTTGAACCTTACG
AGAAAACTTACCGCGTACGCTTTCGCACCGACGGCATTTTGAAAGAAATGTCGCGACCCTCTATAAAGCTGGCACCGAAA
ATATCTGCGCGAATAAAGATCATGGCACAGTTGGACATTTCCGAGCGACGTTTGCCACAAGACGGCCGCATAAAGATGAG
GTTATCCAAAACCAAGGCCATCGACTTTCGGGTGAACACACTGCCCACGCTGTGGGGGGAAAAAATCGTACTGCGAATTC
TGGACCCCAGCCAAGCCAAGCTGGGCATTGATGCGCTTGGGTATGAAGAAGATCAGAAGAAGCTGTATATGGACGCCCTG
GCGCAGCCCCAAGGTATGATTCTGGTGACGGGCCCCACCGGTTCCGGTAAAACCGTATCGTTGTATACTGGCTTGAATAT
TCTAAACACTGACGAGCGCAATATTTCCACGGCGGAAGACCCGGCGGAAATTAACCTGGAAGGCGTAAATCAGGTCAACG
TGAACAACAAGGTAGGGCTTGGCTTTGCCGAAGCGCTAAGAGCGTTTTTGCGGCAAGACCCTGACGTTATCATGGTGGGC
GAGATTCGGGACCTGGAAACCGCTAACATTGCCATCAAAGCGGCACAAACCGGTCACCTTGTGTTGTCCACGTTGCACAC
CAACAGCGCCGCCGAAACCTTAACCCGCATGATGAACATGGGCGTGCCGGCGTTTAACATTGCCACATCTGTTAGCCTGA
TTATTGCCCAGCGCCTGGGCCGGCGACTTTGCAGCTGCAAACAGGCTGGAAGCATCCCGAAAGACGTCCTTTTAGCCGAA
GGGTTCTCAACTGAACAAATTGCAACCGGGTTCACGTTGTACCACCCGAAGGGCTGTGATAAATGCACTAATGGGTATAA
AGGCCGCGTCGGCATTTACGAGGTGGTGAAAGTAACCGAGCAGCTGGCCAACATGATTATGGAAGAAGCCAGTTCTATCA
AGATTGCAAAAGCAGCTCAGGCTGAAGGCTTTCGGACCCTGCGCCAATCCGCCCTGTTAAAGGTGATTGAGGGTTTGACC
AGCCTTGAAGAAGCCAACCGCGTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(195-462)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-152)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB M1FCV5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

56.098

100

0.564
  pilB Acinetobacter baylyi ADP1

54.974

100

0.552
  pilB Legionella pneumophila strain ERS1305867

53.541

100

0.543
  pilB Vibrio campbellii strain DS40M4

50.973

98.949

0.504
  pilB Vibrio parahaemolyticus RIMD 2210633

51.899

96.848

0.503
  pilB Vibrio cholerae strain A1552

53.95

90.893

0.49
  pilF Neisseria gonorrhoeae MS11

47.257

98.949

0.468
  pilB/pilB1 Synechocystis sp. PCC 6803

41.586

90.543

0.377
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.795

92.995

0.361

Multiple sequence alignment