Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   MRBBS_RS04195 Genome accession   NC_018268
Coordinates   926455..927330 (-) Length   291 a.a.
NCBI ID   WP_014870177.1    Uniprot ID   M1FCS8
Organism   Marinobacter sp. BSs20148     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 921455..932330
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MRBBS_RS04165 (MRBBS_0873) - 921823..922752 (-) 930 WP_014870171.1 glucosaminidase domain-containing protein -
  MRBBS_RS04170 (MRBBS_0874) - 922891..923451 (-) 561 WP_014870172.1 YfaZ family outer membrane protein -
  MRBBS_RS04175 (MRBBS_0875) yacG 923665..923862 (-) 198 WP_014870173.1 DNA gyrase inhibitor YacG -
  MRBBS_RS04180 (MRBBS_0876) tsaA 923936..924703 (-) 768 WP_014870174.1 tRNA (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase TrmO -
  MRBBS_RS04185 (MRBBS_0877) - 924709..925818 (-) 1110 WP_187290773.1 hypothetical protein -
  MRBBS_RS04190 (MRBBS_0878) coaE 925790..926398 (-) 609 WP_014870176.1 dephospho-CoA kinase -
  MRBBS_RS04195 (MRBBS_0879) pilD 926455..927330 (-) 876 WP_014870177.1 prepilin peptidase Machinery gene
  MRBBS_RS04200 (MRBBS_0880) pilC 927334..928554 (-) 1221 WP_014870178.1 type II secretion system F family protein Machinery gene
  MRBBS_RS04205 (MRBBS_0881) pilB 928557..930272 (-) 1716 WP_014870179.1 type IV-A pilus assembly ATPase PilB Machinery gene
  MRBBS_RS04210 (MRBBS_0882) - 930578..931045 (+) 468 WP_014870180.1 pilin -

Sequence

Protein


Download         Length: 291 a.a.        Molecular weight: 32110.39 Da        Isoelectric Point: 5.2403

>NTDB_id=52314 MRBBS_RS04195 WP_014870177.1 926455..927330(-) (pilD) [Marinobacter sp. BSs20148]
MVDLSILLDTPWLLYTMVAFVSLCIGSFLNVVIFRLPAMMQQEWRCQCEELLEVPGAKREPAKVLSLSKPASTCPHCGHG
IRAWQNVPVISYLVLKGKCANCSEPISARYPIIELTTAVFSVLTVYLLGPTPAALWALLLVWALVALTMIDFDTQLLPDS
ITLPLLWLGLMVNYFGQLVPFDEAFWGAIVGYLALWSVYWLFKLVTGKEGMGYGDFKLLAALGAWLGWTLLPAVILLSSV
VGAAVGIALMVFKQQGREVPMPFGPYLATAGLLCLWFGDEIVSVWFRFLGV

Nucleotide


Download         Length: 876 bp        

>NTDB_id=52314 MRBBS_RS04195 WP_014870177.1 926455..927330(-) (pilD) [Marinobacter sp. BSs20148]
ATGGTTGATTTAAGCATTCTTCTGGATACACCCTGGTTGCTTTACACCATGGTTGCATTTGTGTCTTTGTGCATCGGCAG
TTTTCTGAACGTCGTTATTTTTCGCCTGCCGGCGATGATGCAGCAGGAGTGGCGCTGCCAGTGTGAAGAGCTGCTGGAAG
TGCCGGGGGCCAAGCGGGAACCGGCGAAGGTGTTGAGCCTGTCAAAGCCAGCGTCTACCTGCCCCCACTGCGGCCACGGT
ATTCGCGCCTGGCAGAATGTACCGGTCATCAGCTACCTGGTGCTGAAAGGCAAATGCGCCAATTGCAGCGAACCGATTTC
AGCGCGCTACCCGATTATTGAGTTAACGACAGCGGTGTTCTCAGTACTTACCGTGTACCTGCTGGGACCAACGCCTGCGG
CGCTTTGGGCGTTATTGCTGGTGTGGGCGCTGGTGGCGCTGACGATGATTGATTTTGATACTCAACTGCTGCCAGACTCC
ATAACCCTGCCCCTGCTTTGGTTAGGGCTGATGGTGAATTACTTTGGCCAGCTGGTGCCTTTTGACGAAGCATTCTGGGG
CGCCATAGTGGGCTACCTGGCTCTCTGGTCAGTTTACTGGCTGTTCAAACTGGTTACCGGCAAGGAAGGCATGGGCTACG
GCGATTTCAAGCTGCTGGCAGCGTTGGGCGCCTGGCTGGGTTGGACGTTATTGCCGGCGGTTATTCTGTTATCCTCTGTT
GTGGGCGCAGCCGTTGGTATAGCCTTGATGGTGTTCAAACAGCAAGGCCGAGAGGTACCCATGCCATTTGGGCCTTATTT
AGCCACAGCCGGCTTGCTGTGCTTATGGTTTGGTGATGAAATTGTGAGTGTGTGGTTCCGCTTTCTTGGTGTATAG

Domains

Predicted by InterproScan.

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(21-126)

Type IV leader peptidase family (Peptidase_A24)

(138-247)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB M1FCS8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Acinetobacter baumannii D1279779

54.412

93.471

0.509
  pilD Acinetobacter nosocomialis M2

54.412

93.471

0.509
  pilD Vibrio campbellii strain DS40M4

52.158

95.533

0.498
  pilD Vibrio cholerae strain A1552

51.254

95.876

0.491
  pilD Neisseria gonorrhoeae MS11

46.975

96.564

0.454

Multiple sequence alignment