Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilG   Type   Machinery gene
Locus tag   RP6297_RS12715 Genome accession   NZ_CP066771
Coordinates   2691505..2692779 (+) Length   424 a.a.
NCBI ID   WP_004633031.1    Uniprot ID   R0E2W3
Organism   Ralstonia pickettii strain K-288     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2686505..2697779
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RP6297_RS12695 (RP6297_02535) ispB 2686977..2687906 (+) 930 WP_050775659.1 octaprenyl diphosphate synthase -
  RP6297_RS12705 (RP6297_02537) - 2688199..2689473 (+) 1275 WP_009277681.1 HlyC/CorC family transporter -
  RP6297_RS12710 (RP6297_02538) pilB 2689733..2691460 (+) 1728 WP_009241590.1 type IV-A pilus assembly ATPase PilB Machinery gene
  RP6297_RS12715 (RP6297_02539) pilG 2691505..2692779 (+) 1275 WP_004633031.1 type II secretion system F family protein Machinery gene
  RP6297_RS12720 (RP6297_02540) pilD 2692784..2693665 (+) 882 WP_009241591.1 A24 family peptidase Machinery gene
  RP6297_RS12725 (RP6297_02541) coaE 2693679..2694293 (+) 615 WP_009241592.1 dephospho-CoA kinase -
  RP6297_RS12730 (RP6297_02542) zapD 2694429..2695187 (+) 759 WP_009241593.1 cell division protein ZapD -
  RP6297_RS12735 (RP6297_02543) - 2695196..2695399 (+) 204 WP_009241594.1 DNA gyrase inhibitor YacG -
  RP6297_RS12740 (RP6297_02544) - 2695392..2695841 (-) 450 WP_009241595.1 NUDIX domain-containing protein -
  RP6297_RS12745 (RP6297_02545) - 2695831..2696715 (-) 885 WP_009241596.1 ATP-binding protein -

Sequence

Protein


Download         Length: 424 a.a.        Molecular weight: 46661.83 Da        Isoelectric Point: 10.3665

>NTDB_id=522461 RP6297_RS12715 WP_004633031.1 2691505..2692779(+) (pilG) [Ralstonia pickettii strain K-288]
MATRAPAANRAAAAPNRTTGKTAKTKAPTQYIFEWEGKDRKGKIFKGEMRAESITEVNAILRKQGLSITKSKRRRAARGK
KITPKDIAYFTRQLSTMLKAGVPLLQSIDIIAKGHANPNFTQLLTEIRVDIESGSSMAQAFRRHPKYFDTLYCNLIDAGE
QGGILDALLERLSLYMEKSLALKAQIKSALIYPISVLTVAFLVTVVLMIFVVPSFKGVFSGFGADLPAPTLFVIAISDFF
VKWWIPIVLGPIAGIALFSRAFKRSENVQRSTHRFILKIPIFGDIIRKATIARWTRTLATMFAAGTPLVESMESVAGAAG
NWIYHDATLEVQQAVRIGTSLTNAMQATHVFDNMVLQMTQIGEESGALDNMLLKVAEFYEREVDDAVANISTLIEPIIIV
FLGVLIGGMVVAMYLPIFKLGTVV

Nucleotide


Download         Length: 1275 bp        

>NTDB_id=522461 RP6297_RS12715 WP_004633031.1 2691505..2692779(+) (pilG) [Ralstonia pickettii strain K-288]
ATGGCCACACGAGCACCCGCCGCGAACCGCGCGGCCGCAGCGCCCAATCGCACGACCGGCAAGACCGCCAAGACCAAGGC
GCCCACGCAGTACATCTTCGAGTGGGAAGGCAAAGATCGGAAAGGCAAGATCTTCAAGGGCGAGATGCGCGCCGAGAGCA
TCACCGAGGTGAATGCCATCCTGCGCAAGCAAGGCCTGTCGATCACCAAATCCAAGCGCCGCCGCGCGGCCCGCGGCAAG
AAGATCACGCCGAAGGACATCGCCTACTTCACGCGCCAGCTGTCGACCATGCTGAAGGCCGGCGTGCCGCTGCTGCAGTC
GATCGACATCATCGCAAAGGGCCACGCCAACCCGAACTTCACGCAGCTGCTCACGGAAATCCGCGTCGATATTGAATCGG
GCAGCAGCATGGCGCAAGCCTTCCGACGTCACCCGAAGTACTTCGACACGCTGTACTGCAACCTGATCGACGCGGGCGAG
CAAGGCGGTATTCTGGATGCGCTGCTCGAGCGGCTTTCGCTGTACATGGAAAAGTCCCTCGCACTGAAGGCGCAGATCAA
GTCGGCCCTGATCTACCCGATCTCGGTGCTGACGGTGGCCTTCCTGGTGACCGTGGTGCTGATGATCTTTGTGGTGCCGT
CGTTCAAGGGTGTTTTCTCCGGTTTCGGTGCCGATTTGCCAGCCCCGACGCTGTTCGTCATTGCGATATCGGATTTCTTC
GTCAAGTGGTGGATACCAATCGTGCTAGGGCCCATTGCCGGCATTGCACTGTTTTCGCGTGCATTCAAGCGATCTGAAAA
CGTCCAGCGTTCGACGCATCGCTTCATCCTGAAGATTCCCATCTTTGGCGACATCATCCGCAAGGCAACCATTGCTCGCT
GGACGCGTACGCTGGCTACCATGTTCGCAGCCGGTACGCCGCTCGTGGAGTCGATGGAGTCGGTCGCCGGGGCCGCAGGC
AACTGGATCTACCATGATGCGACCCTCGAGGTTCAGCAAGCGGTACGTATCGGCACGAGTCTCACCAACGCCATGCAAGC
GACACACGTGTTCGACAACATGGTCCTGCAGATGACGCAGATCGGTGAAGAGTCCGGCGCCCTGGACAACATGCTGCTGA
AGGTGGCGGAGTTCTACGAGCGCGAGGTGGACGATGCGGTCGCCAACATCTCGACTCTGATCGAACCCATCATCATCGTC
TTCCTCGGCGTGCTAATCGGCGGCATGGTGGTGGCGATGTATCTGCCGATCTTCAAGCTTGGTACGGTGGTCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(90-213)

Type II secretion system (T2SS), protein F (T2SSF)

(294-416)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB R0E2W3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilG Neisseria gonorrhoeae MS11

50.873

94.575

0.481
  pilG Neisseria meningitidis 44/76-A

50.623

94.575

0.479
  pilC Pseudomonas stutzeri DSM 10701

49.751

94.811

0.472
  pilC Legionella pneumophila strain ERS1305867

47.772

95.283

0.455
  pilC Acinetobacter baylyi ADP1

46.683

95.991

0.448
  pilC Acinetobacter baumannii D1279779

46.683

95.991

0.448
  pilC Vibrio campbellii strain DS40M4

40.389

96.934

0.392
  pilC Vibrio cholerae strain A1552

40.602

94.104

0.382