Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   RP6297_RS12710 Genome accession   NZ_CP066771
Coordinates   2689733..2691460 (+) Length   575 a.a.
NCBI ID   WP_009241590.1    Uniprot ID   -
Organism   Ralstonia pickettii strain K-288     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2684733..2696460
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RP6297_RS12680 (RP6297_02532) obgE 2684740..2685834 (-) 1095 WP_009241586.1 GTPase ObgE -
  RP6297_RS12685 (RP6297_02533) rpmA 2686032..2686292 (-) 261 WP_009241587.1 50S ribosomal protein L27 -
  RP6297_RS12690 (RP6297_02534) rplU 2686330..2686641 (-) 312 WP_004633023.1 50S ribosomal protein L21 -
  RP6297_RS12695 (RP6297_02535) ispB 2686977..2687906 (+) 930 WP_050775659.1 octaprenyl diphosphate synthase -
  RP6297_RS12705 (RP6297_02537) - 2688199..2689473 (+) 1275 WP_009277681.1 HlyC/CorC family transporter -
  RP6297_RS12710 (RP6297_02538) pilB 2689733..2691460 (+) 1728 WP_009241590.1 type IV-A pilus assembly ATPase PilB Machinery gene
  RP6297_RS12715 (RP6297_02539) pilG 2691505..2692779 (+) 1275 WP_004633031.1 type II secretion system F family protein Machinery gene
  RP6297_RS12720 (RP6297_02540) pilD 2692784..2693665 (+) 882 WP_009241591.1 A24 family peptidase Machinery gene
  RP6297_RS12725 (RP6297_02541) coaE 2693679..2694293 (+) 615 WP_009241592.1 dephospho-CoA kinase -
  RP6297_RS12730 (RP6297_02542) zapD 2694429..2695187 (+) 759 WP_009241593.1 cell division protein ZapD -
  RP6297_RS12735 (RP6297_02543) - 2695196..2695399 (+) 204 WP_009241594.1 DNA gyrase inhibitor YacG -
  RP6297_RS12740 (RP6297_02544) - 2695392..2695841 (-) 450 WP_009241595.1 NUDIX domain-containing protein -

Sequence

Protein


Download         Length: 575 a.a.        Molecular weight: 63718.55 Da        Isoelectric Point: 7.1547

>NTDB_id=522460 RP6297_RS12710 WP_009241590.1 2689733..2691460(+) (pilB) [Ralstonia pickettii strain K-288]
MTLGLALAQSRRIAPTLLTQLEQSAREKRTQLIDELVGSGIMSAHDLAVFVAGKYQMPLLDLAQYKLDTVPAVLSANKHF
AQLRLLPLGRRENRLILATSDPSDPKPIEELRQKMNVAIETVIVEHDKLVRQLSLANEAPAEIKSLFPKQEATQIEYDAG
AAAATRRTTPDGIDDAPVVRFLQKLFTEALLRGASDLHFEPFETFYRVRFRIDGILAQVAQPPLDIRDKIATRIKVLSRL
DISEKRVPQDGRMKLLITVPKDRKDKDNKETIERAIDFRVSTLPTLFGEKIVIRILESSTERLDIDQLGYEPEQKQLLLD
VIKRPYGMVLVTGPTGSGKTVSLYTFLNRLNQGDINISTAEDPAEIQLPGINQVNVNDKAGLTFAAALKSFLRQDPDIIM
VGEIRDLETADISIKAAQTGHLVFSTLHTNDAPTTLTRLMNMGVAPFNIASSVLLITAQRLARRLCKECKKPGPLPKETL
LDAGFTEADLDGSWQPYHPVGCEICNGSGYKGRVGIYQVMPITETIQEIILTHGTALQIAEQARKDGVMSLRESGLRKVK
QGLTSLEEVLATTNQ

Nucleotide


Download         Length: 1728 bp        

>NTDB_id=522460 RP6297_RS12710 WP_009241590.1 2689733..2691460(+) (pilB) [Ralstonia pickettii strain K-288]
ATGACACTCGGACTCGCTCTAGCGCAGAGCCGGCGGATCGCGCCGACGCTACTTACCCAGCTCGAACAGAGCGCGCGTGA
GAAACGCACGCAACTGATCGACGAACTGGTGGGCAGCGGGATCATGAGTGCGCACGACCTCGCCGTGTTCGTGGCCGGCA
AGTACCAGATGCCGCTGCTGGACCTGGCACAGTACAAGCTCGACACCGTGCCGGCCGTGCTGTCGGCCAACAAGCACTTT
GCCCAGTTGCGCCTGTTGCCGCTGGGCCGTCGCGAGAACCGCCTCATCCTCGCCACGTCCGACCCGAGCGATCCGAAGCC
GATCGAAGAGCTGCGTCAGAAGATGAACGTGGCCATCGAAACGGTCATCGTCGAACACGACAAGCTGGTTCGCCAACTCA
GCCTCGCCAATGAGGCGCCGGCCGAGATCAAGTCGCTCTTCCCCAAGCAGGAAGCCACGCAGATCGAATACGATGCGGGC
GCTGCCGCCGCCACGCGTCGCACCACCCCCGACGGCATCGACGATGCCCCTGTGGTGCGCTTCCTGCAGAAGCTCTTTAC
CGAAGCCTTGCTGCGCGGCGCATCGGATTTGCATTTCGAGCCCTTTGAAACGTTTTACCGCGTGCGTTTCCGCATCGACG
GCATCCTCGCGCAGGTCGCGCAGCCGCCGCTCGATATCCGCGACAAGATCGCGACCCGCATCAAGGTGCTCTCGCGCCTC
GACATCTCGGAAAAGCGCGTGCCGCAAGACGGCCGCATGAAGCTGCTCATCACTGTCCCGAAAGACCGCAAGGACAAGGA
CAACAAAGAGACGATCGAGCGCGCCATCGACTTCCGGGTGTCGACGCTGCCGACGCTGTTCGGCGAAAAGATCGTGATCC
GGATTCTGGAGTCGTCCACCGAGCGGCTCGATATCGACCAGCTTGGCTATGAGCCGGAGCAAAAGCAGTTGCTGCTCGAC
GTCATCAAGCGGCCGTACGGCATGGTGCTCGTGACGGGGCCAACCGGATCGGGCAAAACCGTGTCGCTGTACACGTTCCT
GAACAGGCTGAACCAGGGCGACATCAATATCTCCACCGCCGAAGACCCAGCGGAAATCCAGTTGCCCGGCATCAACCAGG
TCAACGTGAACGACAAGGCTGGGCTGACCTTTGCGGCGGCGCTGAAGTCGTTCCTGCGGCAGGATCCGGACATCATCATG
GTGGGCGAAATCCGAGATCTGGAGACGGCCGACATCTCGATCAAGGCTGCGCAGACCGGTCACCTGGTGTTTTCCACGCT
GCACACCAACGACGCACCGACCACACTGACCCGCCTGATGAACATGGGCGTGGCGCCGTTCAACATCGCGTCTTCGGTGC
TACTGATTACGGCGCAGCGTCTGGCGCGCCGGTTGTGCAAGGAATGCAAGAAGCCCGGTCCGCTGCCCAAGGAAACGTTG
CTCGATGCAGGCTTTACCGAGGCAGACCTCGACGGCTCATGGCAGCCGTATCATCCGGTCGGTTGCGAGATCTGCAACGG
CAGCGGTTACAAGGGCCGGGTCGGCATTTACCAGGTCATGCCAATTACCGAAACGATCCAGGAAATCATTCTCACGCATG
GCACGGCGCTGCAGATTGCCGAGCAGGCGCGCAAAGACGGCGTGATGTCGCTACGCGAATCGGGCCTGCGAAAAGTGAAG
CAGGGGCTAACTTCCCTCGAGGAAGTGCTGGCCACAACGAACCAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(184-464)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(36-133)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

52.035

98.261

0.511
  pilF Neisseria gonorrhoeae MS11

53.889

93.913

0.506
  pilB Acinetobacter baylyi ADP1

50.709

98.087

0.497
  pilB Legionella pneumophila strain ERS1305867

46.76

99.304

0.464
  pilB Vibrio cholerae strain A1552

45.487

98.261

0.447
  pilB Vibrio campbellii strain DS40M4

45.438

95.304

0.433
  pilB Vibrio parahaemolyticus RIMD 2210633

45.387

94.261

0.428
  pilF Thermus thermophilus HB27

38.571

97.391

0.376