Detailed information NTG0522462

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: RP6297_RS12720
• Genome accession: NZ_CP066771
• Coordinates: 2692784..2693665 (+)
• Length: 293 a.a.
• NCBI ID: WP_009241591.1
• Uniprot ID: U3G738
• Organism: Ralstonia pickettii strain K-288
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2687784..2698665

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
RP6297_RS12705 (RP6297_02537)	-	2688199..2689473 (+)	1275	WP_009277681.1	HlyC/CorC family transporter	-
RP6297_RS12710 (RP6297_02538)	pilB	2689733..2691460 (+)	1728	WP_009241590.1	type IV-A pilus assembly ATPase PilB	Machinery gene
RP6297_RS12715 (RP6297_02539)	pilG	2691505..2692779 (+)	1275	WP_004633031.1	type II secretion system F family protein	Machinery gene
RP6297_RS12720 (RP6297_02540)	pilD	2692784..2693665 (+)	882	WP_009241591.1	A24 family peptidase	Machinery gene
RP6297_RS12725 (RP6297_02541)	coaE	2693679..2694293 (+)	615	WP_009241592.1	dephospho-CoA kinase	-
RP6297_RS12730 (RP6297_02542)	zapD	2694429..2695187 (+)	759	WP_009241593.1	cell division protein ZapD	-
RP6297_RS12735 (RP6297_02543)	-	2695196..2695399 (+)	204	WP_009241594.1	DNA gyrase inhibitor YacG	-
RP6297_RS12740 (RP6297_02544)	-	2695392..2695841 (-)	450	WP_009241595.1	NUDIX domain-containing protein	-
RP6297_RS12745 (RP6297_02545)	-	2695831..2696715 (-)	885	WP_009241596.1	ATP-binding protein	-
RP6297_RS12750 (RP6297_02546)	argJ	2696732..2697961 (-)	1230	WP_009241597.1	bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ	-

Sequence

Protein

• Length: 293 a.a.
• Molecular weight: 31755.79 Da
• Isoelectric Point: 7.0186

>NTDB_id=522462 RP6297_RS12720 WP_009241591.1 2692784..2693665(+) (pilD) [Ralstonia pickettii strain K-288]
MPEMFVIPTLAQLPAWFVVAAGGLVGLLVGSFLNVVIHRLPRMIEREEANYIAELREEPLPHPEAYNLIVPRSACPSCGH
QIKAIENIPVLSWLALRGRCSACKTPISWRYPAVELVTGLLTAACLWHFGPTWVAVASAVLLWFLIAGTMIDADTQLLPD
AITQPLLWLGLGVNLFDMFTHLHDAVIGAMAGYLFLWSIYWAYKLLRGREGMGYGDFKLMAALGAWFGWQALPLLVLLSS
VVGLVFGLVRIARGAKSESPFSFGPFIAGAGVIALLAGPQLLVMAGLGPLLAP

Nucleotide

• Length: 882 bp

>NTDB_id=522462 RP6297_RS12720 WP_009241591.1 2692784..2693665(+) (pilD) [Ralstonia pickettii strain K-288]
ATGCCTGAGATGTTCGTCATCCCCACGTTGGCGCAACTGCCGGCGTGGTTTGTAGTTGCCGCAGGCGGGCTGGTCGGCTT
GCTGGTCGGCAGCTTCCTGAACGTGGTGATCCACCGCCTGCCGCGCATGATCGAGCGGGAGGAAGCCAACTATATCGCCG
AGTTGCGCGAAGAACCGCTGCCTCACCCGGAGGCATACAACCTGATCGTGCCGCGCTCGGCGTGCCCGTCGTGCGGCCAT
CAGATCAAGGCGATCGAGAATATCCCCGTGTTGAGCTGGCTTGCGCTGCGAGGCCGTTGCAGTGCGTGCAAAACGCCCAT
CTCTTGGCGCTATCCCGCTGTGGAACTGGTCACCGGTCTGCTGACGGCGGCCTGCCTTTGGCACTTCGGCCCGACGTGGG
TAGCGGTTGCATCGGCGGTGCTGCTGTGGTTCCTAATCGCCGGCACGATGATCGACGCCGACACGCAGCTGTTGCCCGAC
GCGATCACCCAGCCGCTGCTGTGGCTGGGGCTGGGCGTGAACCTGTTTGACATGTTCACGCACCTGCACGATGCGGTGAT
CGGCGCGATGGCCGGCTACCTGTTCCTGTGGTCGATCTATTGGGCCTACAAGCTCTTGCGCGGCCGTGAAGGCATGGGCT
ACGGCGACTTCAAGCTGATGGCCGCGCTCGGCGCGTGGTTCGGCTGGCAGGCGCTGCCGCTGCTGGTGCTGCTGTCGTCG
GTCGTGGGGCTGGTGTTTGGGCTCGTGCGCATTGCGCGCGGCGCGAAGAGCGAATCGCCGTTCTCGTTCGGGCCGTTCAT
TGCCGGAGCCGGCGTGATCGCATTGCTGGCCGGCCCGCAATTGCTGGTGATGGCGGGCCTCGGTCCGCTGCTAGCGCCAT
AA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (24-129)

• Type IV leader peptidase family (Peptidase_A24) (140-248)

3D structure

• AlphaFold DB: U3G738

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	54.613	92.491	0.505
pilD	Vibrio campbellii strain DS40M4	50.542	94.539	0.478
pilD	Acinetobacter baumannii D1279779	47.636	93.857	0.447
pilD	Neisseria gonorrhoeae MS11	48.507	91.468	0.444
pilD	Acinetobacter nosocomialis M2	48.864	90.102	0.44