Detailed information NTG0519251

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: GKQ51_RS12055
• Genome accession: NZ_CP066310
• Coordinates: 2560657..2561814 (-)
• Length: 385 a.a.
• NCBI ID: WP_198866122.1
• Uniprot ID: A0AAP9YA68
• Organism: Azotobacter chroococcum strain HR1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2555657..2566814

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GKQ51_RS12035 (GKQ51_12035)	-	2555711..2557435 (-)	1725	WP_198866118.1	autotransporter assembly complex protein TamA	-
GKQ51_RS12040 (GKQ51_12040)	-	2557511..2558149 (-)	639	WP_198866119.1	GNAT family N-acetyltransferase	-
GKQ51_RS12045 (GKQ51_12045)	xthA	2558285..2559097 (+)	813	WP_198866120.1	exodeoxyribonuclease III	-
GKQ51_RS12050 (GKQ51_12050)	-	2559232..2560611 (+)	1380	WP_198866121.1	substrate-binding domain-containing protein	-
GKQ51_RS12055 (GKQ51_12055)	pilU	2560657..2561814 (-)	1158	WP_198866122.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
GKQ51_RS12060 (GKQ51_12060)	-	2561937..2562215 (+)	279	WP_165891648.1	peptidylprolyl isomerase	-
GKQ51_RS12065 (GKQ51_12065)	-	2562387..2563616 (+)	1230	WP_198866123.1	MFS transporter	-
GKQ51_RS12070 (GKQ51_12070)	-	2564265..2564441 (+)	177	WP_198866124.1	hypothetical protein	-

Sequence

Protein

• Length: 385 a.a.
• Molecular weight: 42586.71 Da
• Isoelectric Point: 6.3262

>NTDB_id=519251 GKQ51_RS12055 WP_198866122.1 2560657..2561814(-) (pilU) [Azotobacter chroococcum strain HR1]
MDIQSMLRVLATQDGSDLYLSTGAPPCAKFNGVLKALGQEPLQPGAVAKIAEGLMDAEQRVEFERELEMNLAISLPNIGR
FRINIFKQRNEVSIVARNIKLDIPRFEDLKLPKILLDVVMEKRGLVLFVGGTGSGKSTSLAALIDYRNRNSGGHIITIED
PVEYVHRHKKSIINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFADTGHLAISTLHANNANQALDRI
INFFPEERRPQLLNDLGNNLKAFVSQRLVKTVDGKRRAAVEVLLGTPTVRDLIKRSELSEIKEIMEKSRNLGMQTFDQAL
IELVNEGAIGEEEALKNADSANNVRLKLKLHRDGGVAGADQKGGESPWQGLSLEMVEEPEEAGNS

Nucleotide

• Length: 1158 bp

>NTDB_id=519251 GKQ51_RS12055 WP_198866122.1 2560657..2561814(-) (pilU) [Azotobacter chroococcum strain HR1]
ATGGATATTCAATCGATGCTGAGAGTCCTGGCCACCCAGGACGGTTCGGACCTTTACCTGTCCACCGGCGCGCCGCCCTG
CGCGAAGTTCAACGGCGTGCTCAAGGCGCTCGGCCAGGAGCCGCTGCAGCCGGGCGCCGTGGCGAAGATCGCCGAGGGGC
TGATGGATGCCGAGCAGCGCGTCGAGTTCGAGCGCGAGCTGGAAATGAACCTGGCGATCTCCCTGCCGAACATCGGCCGT
TTCCGCATCAACATCTTCAAGCAGCGCAACGAGGTGTCCATCGTCGCGCGCAACATCAAGCTGGACATTCCGCGCTTCGA
GGATCTCAAGCTGCCGAAGATCCTCCTCGACGTGGTGATGGAGAAGCGCGGCCTGGTGCTGTTCGTCGGCGGCACCGGCT
CGGGCAAGTCGACCTCCCTGGCGGCGCTGATCGACTACCGCAACCGCAACAGCGGCGGGCACATCATCACCATCGAGGAT
CCGGTGGAATACGTGCACCGGCACAAGAAGTCGATCATCAACCAGCGCGAGGTCGGGGTGGACACCCGCAGCTTCCACGC
CGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCCTGATCGGCGAGATCCGCGACCGCGAGACCATGGAGCACG
CCCTGGCCTTCGCCGACACCGGCCACCTGGCAATTTCCACGCTGCACGCCAACAACGCCAATCAGGCGCTGGACCGCATC
ATCAACTTCTTCCCCGAGGAGCGCCGACCGCAACTGCTCAACGACCTGGGCAACAACCTCAAGGCCTTCGTCTCCCAGCG
CCTGGTCAAGACGGTCGACGGCAAGCGCCGGGCCGCGGTGGAGGTGCTGCTCGGCACGCCGACGGTGCGTGACCTGATCA
AGCGCAGCGAGCTGTCCGAGATCAAGGAGATCATGGAGAAGTCACGCAACCTCGGCATGCAGACCTTCGACCAGGCGCTG
ATCGAGCTGGTCAACGAGGGCGCCATCGGCGAGGAGGAGGCGCTGAAGAACGCCGACTCGGCGAACAACGTGCGCCTCAA
GCTGAAGCTGCACCGCGACGGCGGGGTAGCCGGCGCCGACCAGAAGGGCGGGGAAAGCCCTTGGCAGGGGCTGAGTCTGG
AGATGGTCGAGGAGCCGGAGGAGGCCGGCAATAGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (112-248)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	54.617	98.442	0.538
pilU	Acinetobacter baylyi ADP1	51.989	91.429	0.475
pilU	Vibrio cholerae strain A1552	51.558	91.688	0.473
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.052	0.379
pilT	Legionella pneumophila strain Lp02	42.515	86.753	0.369
pilT	Legionella pneumophila strain ERS1305867	42.515	86.753	0.369
pilT	Vibrio cholerae strain A1552	41.742	86.494	0.361
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	41.742	86.494	0.361
pilT	Pseudomonas aeruginosa PAK	40.525	89.091	0.361