Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   GKQ51_RS05035 Genome accession   NZ_CP066310
Coordinates   1077739..1079439 (-) Length   566 a.a.
NCBI ID   WP_198867368.1    Uniprot ID   A0AAP9YFH9
Organism   Azotobacter chroococcum strain HR1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1072739..1084439
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GKQ51_RS05005 (GKQ51_05005) - 1073100..1073567 (+) 468 WP_198867367.1 FAD/FMN-containing dehydrogenase -
  GKQ51_RS05010 (GKQ51_05010) - 1073564..1074250 (+) 687 WP_089167356.1 energy-coupling factor ABC transporter permease -
  GKQ51_RS05015 (GKQ51_05015) yacG 1074271..1074471 (-) 201 WP_089167357.1 DNA gyrase inhibitor YacG -
  GKQ51_RS05020 (GKQ51_05020) coaE 1074468..1075076 (-) 609 WP_089167358.1 dephospho-CoA kinase -
  GKQ51_RS05025 (GKQ51_05025) pilD 1075225..1076094 (-) 870 WP_089167359.1 prepilin peptidase Machinery gene
  GKQ51_RS05030 (GKQ51_05030) pilC 1076097..1077314 (-) 1218 WP_089169794.1 type II secretion system F family protein Machinery gene
  GKQ51_RS05035 (GKQ51_05035) pilB 1077739..1079439 (-) 1701 WP_198867368.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GKQ51_RS05040 (GKQ51_05040) pilA2 1079991..1080437 (+) 447 WP_198867369.1 pilin Machinery gene
  GKQ51_RS05045 (GKQ51_05045) - 1080644..1081186 (+) 543 WP_198867370.1 REP-associated tyrosine transposase -
  GKQ51_RS05050 (GKQ51_05050) - 1081222..1081608 (-) 387 WP_198867371.1 type II toxin-antitoxin system VapC family toxin -
  GKQ51_RS05055 (GKQ51_05055) - 1081605..1081850 (-) 246 WP_198867372.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
  GKQ51_RS05060 (GKQ51_05060) nadC 1082097..1082945 (-) 849 WP_131300091.1 carboxylating nicotinate-nucleotide diphosphorylase -
  GKQ51_RS05065 (GKQ51_05065) ampD 1083098..1083661 (+) 564 WP_039805672.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62313.33 Da        Isoelectric Point: 5.6466

>NTDB_id=519244 GKQ51_RS05035 WP_198867368.1 1077739..1079439(-) (pilB) [Azotobacter chroococcum strain HR1]
MSNDISLPGLARQMVLAGLIDEKTAQQAQQQAQRNQTPLVTWLVQNKLVRSRALAELAAEQFGIALFDLKTLDRESQPRD
LLSEKLIRQHRTLPLWRRGNRLFVAISDPTNHEAIREIRFGTGLNTEAVLVEDDRLGEAIEKYFEGAGTGLDDLADAELD
GLDTEAASQKDDEADPSGGAEDAPVVRFVNKMLLDAIRGGSSDLHFEPYEKSYRVRLRTDGILHEVARPPVQLAPKIAAR
LKVMAGLDISERRKPQDGRIKMKLSKTKAIDFRVNTLPTLWGEKTVMRILDPSSAQMGIDALGYEPEQKALYLAALSQPQ
GMILVTGPTGSGKTVSLYTGLNILNTPEVNISTAEDPVEINLEGINQVNVNPRQGMDFSQALRAFLRQDPDIIMVGEIRD
LETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLRNMGVPSFNIATSVNLIIAQRLARKLCACKKAVDIPREALLEEGFPE
ARIGTFKVYAPVGCENCNGGYKGRVGIYEVVKITPALQRIIMEDGNSIEIARQMRADGFNDLRTSALLKAMQGVTSLEEV
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=519244 GKQ51_RS05035 WP_198867368.1 1077739..1079439(-) (pilB) [Azotobacter chroococcum strain HR1]
ATGAGCAACGACATTTCCCTCCCCGGCCTGGCACGGCAGATGGTGCTGGCCGGGCTGATCGACGAGAAGACCGCCCAGCA
GGCCCAGCAGCAGGCACAGCGCAACCAGACTCCCTTGGTCACCTGGCTGGTGCAGAACAAGCTGGTCAGGAGCCGGGCGC
TGGCGGAACTGGCCGCCGAGCAGTTCGGCATCGCCCTGTTCGACCTCAAGACGCTGGACCGGGAGAGCCAGCCCCGGGAT
CTGCTCAGCGAAAAGCTGATCCGCCAGCACCGCACCCTGCCGCTGTGGCGGCGCGGCAACCGGCTGTTCGTGGCGATCTC
CGACCCAACCAACCACGAGGCGATCCGCGAGATCCGCTTCGGCACCGGGCTGAACACCGAGGCCGTGCTGGTCGAGGACG
ACCGACTGGGCGAGGCCATCGAGAAATATTTCGAGGGCGCCGGCACCGGCCTGGACGATCTGGCCGATGCCGAGCTGGAT
GGCCTGGACACCGAGGCGGCCTCCCAGAAGGACGACGAGGCCGACCCGAGCGGCGGCGCCGAGGATGCGCCGGTGGTGCG
CTTCGTCAACAAGATGCTGCTGGATGCGATCCGCGGCGGCTCCTCGGACCTGCACTTCGAACCCTACGAGAAGAGCTACC
GGGTGCGCCTTCGTACCGACGGCATCCTCCACGAGGTGGCCAGGCCGCCGGTGCAGCTGGCGCCGAAGATCGCCGCGCGC
CTCAAGGTGATGGCCGGGCTGGACATCTCCGAGCGGCGCAAGCCGCAGGACGGCCGGATCAAGATGAAGCTGTCGAAGAC
CAAGGCCATCGACTTCCGGGTCAACACCCTGCCGACCCTGTGGGGCGAGAAGACCGTGATGCGGATTCTCGACCCGTCCA
GCGCGCAGATGGGCATCGACGCCCTGGGCTACGAGCCGGAGCAGAAGGCGCTCTACCTGGCGGCGCTGAGCCAGCCGCAG
GGGATGATCCTGGTGACCGGCCCGACCGGCTCGGGCAAGACAGTGTCGCTGTACACCGGCCTGAACATCCTCAACACCCC
GGAGGTGAACATCTCCACCGCGGAAGACCCGGTGGAGATCAACCTGGAGGGCATCAACCAGGTCAACGTCAATCCGCGCC
AGGGCATGGACTTCTCCCAGGCGCTGCGCGCCTTCCTGCGCCAGGACCCGGACATCATCATGGTTGGGGAGATCCGCGAC
CTGGAAACCGCCGAGATCGCCATCAAGGCCGCACAGACCGGGCACATGGTGATGTCCACCCTGCACACCAACAGCGCCGC
GGAAACTCTGACCCGCCTGCGCAACATGGGCGTGCCCTCGTTCAACATCGCCACCTCGGTGAACCTGATCATCGCCCAGC
GCCTGGCGCGCAAGCTGTGCGCCTGCAAGAAGGCGGTGGACATTCCCCGCGAGGCGCTGCTCGAGGAGGGCTTTCCGGAG
GCGCGCATCGGCACCTTCAAGGTCTACGCCCCGGTCGGTTGCGAGAACTGCAACGGCGGCTACAAGGGCCGGGTCGGCAT
TTATGAAGTGGTTAAAATCACCCCGGCCCTGCAGCGCATTATCATGGAAGACGGCAACTCCATCGAAATTGCCCGGCAGA
TGCGCGCCGACGGCTTCAACGACCTGCGCACATCCGCCCTGCTGAAAGCCATGCAGGGTGTCACCAGCCTGGAAGAAGTC
AACCGCGTCACCAAGGACTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-146)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

61.702

99.647

0.615
  pilB Acinetobacter baylyi ADP1

59.68

99.47

0.594
  pilB Legionella pneumophila strain ERS1305867

54.69

99.823

0.546
  pilB Vibrio cholerae strain A1552

51.259

98.233

0.504
  pilB Vibrio parahaemolyticus RIMD 2210633

50.271

97.703

0.491
  pilF Neisseria gonorrhoeae MS11

49.023

99.47

0.488
  pilB Vibrio campbellii strain DS40M4

48.845

99.47

0.486
  pilB/pilB1 Synechocystis sp. PCC 6803

37.52

100

0.406
  pilF Thermus thermophilus HB27

38.83

99.647

0.387
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.189

93.64

0.376