Detailed information NTG0519242

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: GKQ51_RS05025
• Genome accession: NZ_CP066310
• Coordinates: 1075225..1076094 (-)
• Length: 289 a.a.
• NCBI ID: WP_089167359.1
• Uniprot ID: -
• Organism: Azotobacter chroococcum strain HR1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1070225..1081094

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GKQ51_RS04990 (GKQ51_04990)	-	1070829..1072127 (-)	1299	WP_089167353.1	NAD(P)/FAD-dependent oxidoreductase	-
GKQ51_RS04995 (GKQ51_04995)	-	1072191..1072364 (-)	174	WP_039805690.1	DUF3094 family protein	-
GKQ51_RS05000 (GKQ51_05000)	-	1072678..1073103 (+)	426	WP_198867366.1	GNAT family N-acetyltransferase	-
GKQ51_RS05005 (GKQ51_05005)	-	1073100..1073567 (+)	468	WP_198867367.1	FAD/FMN-containing dehydrogenase	-
GKQ51_RS05010 (GKQ51_05010)	-	1073564..1074250 (+)	687	WP_089167356.1	energy-coupling factor ABC transporter permease	-
GKQ51_RS05015 (GKQ51_05015)	yacG	1074271..1074471 (-)	201	WP_089167357.1	DNA gyrase inhibitor YacG	-
GKQ51_RS05020 (GKQ51_05020)	coaE	1074468..1075076 (-)	609	WP_089167358.1	dephospho-CoA kinase	-
GKQ51_RS05025 (GKQ51_05025)	pilD	1075225..1076094 (-)	870	WP_089167359.1	prepilin peptidase	Machinery gene
GKQ51_RS05030 (GKQ51_05030)	pilC	1076097..1077314 (-)	1218	WP_089169794.1	type II secretion system F family protein	Machinery gene
GKQ51_RS05035 (GKQ51_05035)	pilB	1077739..1079439 (-)	1701	WP_198867368.1	type IV-A pilus assembly ATPase PilB	Machinery gene
GKQ51_RS05040 (GKQ51_05040)	pilA2	1079991..1080437 (+)	447	WP_198867369.1	pilin	Machinery gene

Sequence

Protein

• Length: 289 a.a.
• Molecular weight: 31599.54 Da
• Isoelectric Point: 7.1351

>NTDB_id=519242 GKQ51_RS05025 WP_089167359.1 1075225..1076094(-) (pilD) [Azotobacter chroococcum strain HR1]
MSIHDLLAGPGPAFAFWAGLLGLVVGSFLNVVIHRLPKMMLRDWQAQAREVLELPGEAPGEPFNLLLPHSHCPHCGYQIR
PWENIPLLGWLALRGRCSACKAPIGARYPLVELACGLLSAHVAWHYGFGWQAGAMLLLGWGLLAMSLIDYDHQLLPDSLV
LPLLWLGLILNNFGLFVSLEDALWGAVAGYLSLWSVYWLFKLLTGKEGMGYGDFKLLAMLGAWGGWQILPLTILLSSLVG
AVLGIVILRLRKAESGTPIPFGPFLALAGWIALLWGERITGSYLQFAGL

Nucleotide

• Length: 870 bp

>NTDB_id=519242 GKQ51_RS05025 WP_089167359.1 1075225..1076094(-) (pilD) [Azotobacter chroococcum strain HR1]
ATGTCCATTCACGATCTTCTCGCCGGTCCCGGGCCGGCCTTCGCTTTCTGGGCGGGGCTGCTGGGCTTGGTGGTCGGCAG
CTTTCTCAACGTGGTGATCCATCGCCTGCCGAAGATGATGCTGCGCGACTGGCAGGCGCAGGCGCGGGAAGTGCTGGAAC
TGCCCGGCGAGGCACCGGGTGAGCCCTTCAACCTGCTGCTGCCCCACTCGCATTGCCCGCACTGCGGATACCAGATCCGC
CCCTGGGAGAACATCCCGCTGCTCGGCTGGCTGGCCCTGCGCGGCCGCTGCTCGGCCTGCAAGGCGCCGATCGGCGCACG
CTATCCGCTGGTGGAGCTGGCTTGCGGACTACTCTCGGCTCATGTCGCCTGGCATTACGGCTTTGGTTGGCAGGCCGGCG
CCATGCTGCTGCTCGGCTGGGGCCTCTTGGCCATGAGCCTGATCGACTACGATCATCAGCTGCTGCCGGACTCGCTGGTG
CTGCCGCTGCTCTGGCTAGGGCTGATCCTCAACAACTTCGGGCTGTTCGTTTCCCTGGAGGATGCCCTGTGGGGCGCGGT
AGCCGGCTATCTCAGCCTGTGGTCGGTGTACTGGCTGTTCAAGCTGCTCACCGGCAAGGAAGGCATGGGCTACGGCGACT
TCAAGCTGCTGGCGATGCTCGGCGCCTGGGGCGGCTGGCAGATCCTGCCGCTGACCATCCTGCTCTCCTCGCTGGTCGGC
GCCGTGCTGGGAATCGTCATCCTGCGCCTGCGCAAGGCCGAAAGCGGCACGCCAATCCCCTTCGGCCCCTTCCTCGCCCT
CGCCGGCTGGATCGCCCTGCTCTGGGGCGAGCGGATCACCGGCAGCTACCTGCAGTTCGCCGGTTTGTAG

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (137-245)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (20-126)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	57.664	94.81	0.547
pilD	Vibrio campbellii strain DS40M4	53.846	94.464	0.509
pilD	Acinetobacter baumannii D1279779	50.37	93.426	0.471
pilD	Acinetobacter nosocomialis M2	50.37	93.426	0.471
pilD	Neisseria gonorrhoeae MS11	49.81	91.003	0.453