Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   GKQ51_RS05030 Genome accession   NZ_CP066310
Coordinates   1076097..1077314 (-) Length   405 a.a.
NCBI ID   WP_089169794.1    Uniprot ID   A0AAP9YIR0
Organism   Azotobacter chroococcum strain HR1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1071097..1082314
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GKQ51_RS04995 (GKQ51_04995) - 1072191..1072364 (-) 174 WP_039805690.1 DUF3094 family protein -
  GKQ51_RS05000 (GKQ51_05000) - 1072678..1073103 (+) 426 WP_198867366.1 GNAT family N-acetyltransferase -
  GKQ51_RS05005 (GKQ51_05005) - 1073100..1073567 (+) 468 WP_198867367.1 FAD/FMN-containing dehydrogenase -
  GKQ51_RS05010 (GKQ51_05010) - 1073564..1074250 (+) 687 WP_089167356.1 energy-coupling factor ABC transporter permease -
  GKQ51_RS05015 (GKQ51_05015) yacG 1074271..1074471 (-) 201 WP_089167357.1 DNA gyrase inhibitor YacG -
  GKQ51_RS05020 (GKQ51_05020) coaE 1074468..1075076 (-) 609 WP_089167358.1 dephospho-CoA kinase -
  GKQ51_RS05025 (GKQ51_05025) pilD 1075225..1076094 (-) 870 WP_089167359.1 prepilin peptidase Machinery gene
  GKQ51_RS05030 (GKQ51_05030) pilC 1076097..1077314 (-) 1218 WP_089169794.1 type II secretion system F family protein Machinery gene
  GKQ51_RS05035 (GKQ51_05035) pilB 1077739..1079439 (-) 1701 WP_198867368.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GKQ51_RS05040 (GKQ51_05040) pilA2 1079991..1080437 (+) 447 WP_198867369.1 pilin Machinery gene
  GKQ51_RS05045 (GKQ51_05045) - 1080644..1081186 (+) 543 WP_198867370.1 REP-associated tyrosine transposase -
  GKQ51_RS05050 (GKQ51_05050) - 1081222..1081608 (-) 387 WP_198867371.1 type II toxin-antitoxin system VapC family toxin -
  GKQ51_RS05055 (GKQ51_05055) - 1081605..1081850 (-) 246 WP_198867372.1 type II toxin-antitoxin system Phd/YefM family antitoxin -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44224.92 Da        Isoelectric Point: 9.9229

>NTDB_id=519243 GKQ51_RS05030 WP_089169794.1 1076097..1077314(-) (pilC) [Azotobacter chroococcum strain HR1]
MAEKALKTSLFTWEGTDRRGAKVKGELSGQNPALVKAQLRKQGINPTRVRKKAASLFGAGKKIKPLDIALFTRQMATMMK
AGVPLLQAFDIISEGFDNPNMRTLVDEVKQGVAAGNSFASALRKKPQYFDDLYCNLVDSGEQSGALENLLDRVATYKEKT
EALKAKIKKAMNYPIAVIVVAIIVSAILLIKVVPQFESVFANFGAELPAFTRMVIRMSEVMQAYWFYGLLGLFAIAFTLK
QAHQRSEKFRNWTDRTLLKLPIVGEILYKSAVARFARTLATTFAAGVPLVEALDSVSGATGNVVFRTATEKVKADVTTGM
QLNFSMRTTGTFPTMAIQMTAIGEESGALDEMLDKVASFYEAEVDNMVDGLTSLMEPMIMAVLGVLVGGLIIAMYLPIFQ
LGAVV

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=519243 GKQ51_RS05030 WP_089169794.1 1076097..1077314(-) (pilC) [Azotobacter chroococcum strain HR1]
ATGGCGGAAAAAGCGTTAAAGACCAGTTTGTTCACCTGGGAGGGCACCGACCGGCGCGGCGCCAAGGTCAAGGGCGAGCT
GAGCGGGCAGAATCCGGCGCTGGTCAAGGCGCAGCTGCGCAAGCAGGGCATCAACCCGACCAGGGTGCGCAAGAAGGCCG
CCTCGCTGTTCGGCGCCGGCAAGAAGATCAAGCCGCTGGACATCGCCCTGTTCACCCGGCAGATGGCCACCATGATGAAA
GCCGGCGTGCCGCTGCTGCAGGCCTTCGACATCATCTCCGAGGGTTTCGACAACCCGAACATGCGCACGCTGGTGGATGA
AGTGAAGCAGGGAGTGGCGGCCGGCAACAGCTTCGCCAGCGCCCTGCGCAAGAAGCCGCAGTACTTCGACGACCTCTACT
GCAACCTAGTGGACTCCGGCGAGCAGTCCGGCGCCCTGGAAAACCTGCTGGACCGGGTCGCCACCTACAAGGAGAAGACC
GAGGCGCTGAAGGCCAAGATCAAGAAGGCGATGAACTACCCCATCGCGGTGATCGTGGTGGCGATCATCGTCTCGGCGAT
CCTGCTGATCAAGGTGGTGCCGCAGTTCGAATCGGTGTTCGCCAATTTCGGCGCCGAGCTGCCGGCCTTCACCCGCATGG
TCATCCGCATGTCCGAGGTCATGCAGGCATACTGGTTCTACGGGTTGTTGGGGCTATTCGCCATAGCCTTCACCCTCAAG
CAGGCGCACCAGCGCTCGGAGAAGTTCCGCAACTGGACGGACCGCACCCTGCTCAAGCTGCCGATCGTCGGCGAGATCCT
CTACAAGTCGGCGGTGGCCCGTTTCGCCCGCACCCTGGCCACCACCTTCGCCGCCGGCGTGCCGCTGGTCGAGGCGCTGG
ACTCGGTGTCCGGCGCCACCGGCAACGTGGTGTTCCGCACCGCCACCGAGAAGGTCAAGGCCGATGTCACCACCGGCATG
CAGCTGAACTTCTCCATGCGCACCACCGGCACCTTTCCGACCATGGCGATCCAGATGACCGCCATCGGCGAGGAGTCCGG
CGCGCTCGACGAGATGCTCGACAAGGTGGCGAGCTTCTACGAGGCCGAAGTGGACAACATGGTGGACGGCCTGACCAGCC
TGATGGAGCCGATGATCATGGCGGTGCTCGGTGTGCTGGTCGGCGGTCTGATCATCGCCATGTACCTACCGATCTTCCAG
CTGGGCGCGGTGGTTTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(275-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

82.222

100

0.822
  pilC Acinetobacter baylyi ADP1

61.33

100

0.615
  pilC Acinetobacter baumannii D1279779

60.247

100

0.602
  pilC Legionella pneumophila strain ERS1305867

54.774

98.272

0.538
  pilG Neisseria meningitidis 44/76-A

45.906

99.506

0.457
  pilG Neisseria gonorrhoeae MS11

45.906

99.506

0.457
  pilC Vibrio cholerae strain A1552

42.222

100

0.422
  pilC Vibrio campbellii strain DS40M4

41.919

97.778

0.41
  pilC Thermus thermophilus HB27

36.779

100

0.378