Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   I9018_RS27695 Genome accession   NZ_CP066123
Coordinates   6043503..6044720 (+) Length   405 a.a.
NCBI ID   WP_241108037.1    Uniprot ID   -
Organism   Pseudomonas sp. MPFS     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6038503..6049720
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I9018_RS27680 (I9018_27590) - 6039121..6042039 (+) 2919 WP_241108036.1 DUF748 domain-containing protein -
  I9018_RS27685 (I9018_27595) - 6042240..6042596 (-) 357 WP_068575524.1 BON domain-containing protein -
  I9018_RS27690 (I9018_27600) - 6042812..6043228 (-) 417 WP_124347366.1 pilin -
  I9018_RS27695 (I9018_27605) pilC 6043503..6044720 (+) 1218 WP_241108037.1 type II secretion system F family protein Machinery gene
  I9018_RS27700 (I9018_27610) pilD 6044723..6045595 (+) 873 WP_241108038.1 A24 family peptidase Machinery gene
  I9018_RS27705 (I9018_27615) coaE 6045592..6046215 (+) 624 WP_241108039.1 dephospho-CoA kinase -
  I9018_RS27710 (I9018_27620) yacG 6046212..6046415 (+) 204 WP_068575529.1 DNA gyrase inhibitor YacG -
  I9018_RS27715 (I9018_27625) - 6046433..6046648 (-) 216 WP_022641062.1 hypothetical protein -
  I9018_RS27720 (I9018_27630) - 6046712..6047401 (-) 690 WP_068575530.1 energy-coupling factor ABC transporter permease -
  I9018_RS27725 (I9018_27635) - 6047398..6047865 (-) 468 WP_068575531.1 hypothetical protein -
  I9018_RS27730 (I9018_27640) - 6048018..6048647 (+) 630 WP_068575532.1 DUF1780 domain-containing protein -
  I9018_RS27735 (I9018_27645) - 6048836..6049009 (+) 174 WP_068575533.1 DUF3094 family protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 43931.60 Da        Isoelectric Point: 10.4679

>NTDB_id=517551 I9018_RS27695 WP_241108037.1 6043503..6044720(+) (pilC) [Pseudomonas sp. MPFS]
MAVKASRTSVYLWQGTDSRGHVMSGELSAANPALVGARLRKQGIIPTRVRRKPRYGLRLGPRVKSRDIALLTRQLATLFQ
AGVPLLQSLEIIADSFGQPLIRQLLGQLKHDIEAGSNLANALRKHPQHFDPLYCNLVEAGEQAGALDTLLERIATYQEKS
ERLKARLKKAMVYPLAVIGVALIVSSILLLKVVPQFQSLFAGFNAELPWLTQKVIGLSYFLQQHGGTSLAACLGLGLGGR
LLYRRSARLRDHLDAGLLRLPLGGALLHKSAVARMARTLATTFAAGVPLVQALESVAGASGNHIHRRAMERIRLQVEGGM
QLNFAMRASGVFPDMAIQMTAIGEESGTLDHLLEKVAAHYEAEVDNLVDSLTSLMEPLIMVILGSIVAALVLAMYLPIFQ
LGTAI

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=517551 I9018_RS27695 WP_241108037.1 6043503..6044720(+) (pilC) [Pseudomonas sp. MPFS]
ATGGCGGTAAAAGCTTCCAGGACCAGCGTGTACCTCTGGCAAGGTACCGATAGCCGGGGTCATGTCATGAGCGGCGAACT
GAGTGCCGCGAACCCGGCCCTGGTTGGGGCTCGCCTACGCAAGCAGGGCATCATCCCAACCAGGGTCCGCCGCAAACCTC
GGTACGGGCTGCGTTTGGGACCGCGGGTAAAATCACGGGACATCGCTTTGCTCACCCGCCAATTGGCAACGCTGTTCCAG
GCTGGCGTACCACTGCTGCAGTCCCTGGAAATCATTGCCGATAGCTTCGGCCAACCGTTGATCCGCCAGCTCCTGGGACA
ACTCAAGCACGATATCGAGGCCGGCTCCAACCTGGCCAACGCGCTGCGCAAACACCCACAACACTTCGACCCGTTGTACT
GCAACCTGGTGGAGGCCGGCGAGCAGGCAGGGGCCCTGGATACACTGCTGGAGCGGATCGCCACCTACCAGGAAAAAAGC
GAGCGTCTCAAGGCCCGCCTGAAGAAGGCCATGGTCTACCCCCTGGCAGTGATCGGCGTCGCGCTCATAGTCAGCAGCAT
CCTGCTGCTCAAAGTGGTTCCGCAATTCCAGAGTCTGTTCGCCGGCTTCAATGCCGAACTGCCCTGGCTGACCCAGAAGG
TCATCGGTCTTTCATATTTCCTGCAGCAACACGGTGGAACCTCGCTGGCAGCCTGCCTGGGGCTCGGGCTAGGTGGACGC
CTGCTCTATCGTCGTTCTGCACGTCTACGCGATCATCTGGATGCAGGGTTGTTGCGCTTGCCACTGGGCGGCGCCTTGCT
GCACAAGTCTGCCGTGGCGCGCATGGCCCGTACCCTGGCCACCACCTTCGCTGCGGGAGTCCCCCTGGTTCAAGCCCTGG
AGTCCGTCGCTGGTGCCAGCGGCAACCATATTCATCGACGCGCGATGGAGCGTATCCGCCTCCAGGTCGAAGGTGGCATG
CAACTGAATTTCGCCATGCGTGCCAGCGGTGTTTTCCCTGACATGGCGATCCAGATGACCGCCATCGGTGAAGAATCCGG
AACCCTGGATCATTTATTGGAAAAGGTAGCGGCGCATTATGAAGCTGAGGTGGATAACCTGGTGGACAGCCTGACCAGCC
TCATGGAGCCTTTGATCATGGTGATCCTGGGCAGTATCGTCGCAGCCTTGGTACTGGCGATGTACTTGCCCATTTTTCAA
CTCGGTACAGCGATCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)

Type II secretion system (T2SS), protein F (T2SSF)

(72-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

56.296

100

0.563
  pilC Acinetobacter baylyi ADP1

51.834

100

0.523
  pilC Acinetobacter baumannii D1279779

51.716

100

0.521
  pilC Legionella pneumophila strain ERS1305867

47.355

98.025

0.464
  pilC Vibrio cholerae strain A1552

43.367

96.79

0.42
  pilG Neisseria gonorrhoeae MS11

41.687

99.506

0.415
  pilG Neisseria meningitidis 44/76-A

41.191

99.506

0.41
  pilC Vibrio campbellii strain DS40M4

41.327

96.79

0.4
  pilC Thermus thermophilus HB27

36.75

98.765

0.363