Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB/cglB   Type   Machinery gene
Locus tag   I6I32_RS04365 Genome accession   NZ_CP066041
Coordinates   902014..903030 (-) Length   338 a.a.
NCBI ID   WP_198465068.1    Uniprot ID   -
Organism   Streptococcus oralis strain FDAARGOS_1075     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 897014..908030
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6I32_RS04325 (I6I32_04325) - 897348..898538 (-) 1191 WP_000167789.1 acetate kinase -
  I6I32_RS04330 (I6I32_04330) comYH 898590..899543 (-) 954 WP_198464998.1 class I SAM-dependent methyltransferase Machinery gene
  I6I32_RS04335 (I6I32_04335) - 899602..900192 (-) 591 WP_198464999.1 class I SAM-dependent methyltransferase -
  I6I32_RS04340 (I6I32_04340) comGG/cglG 900197..900625 (-) 429 WP_049478131.1 competence type IV pilus minor pilin ComGG Machinery gene
  I6I32_RS04345 (I6I32_04345) comGF/cglF 900603..901064 (-) 462 WP_198465000.1 competence type IV pilus minor pilin ComGF Machinery gene
  I6I32_RS04350 (I6I32_04350) comGE/cglE 901027..901329 (-) 303 WP_000413375.1 competence type IV pilus minor pilin ComGE Machinery gene
  I6I32_RS04355 (I6I32_04355) comGD/cglD 901292..901726 (-) 435 WP_198465001.1 competence type IV pilus minor pilin ComGD Machinery gene
  I6I32_RS04360 (I6I32_04360) comGC/cglC 901689..902012 (-) 324 WP_000735798.1 competence type IV pilus major pilin ComGC Machinery gene
  I6I32_RS04365 (I6I32_04365) comGB/cglB 902014..903030 (-) 1017 WP_198465068.1 competence type IV pilus assembly protein ComGB Machinery gene
  I6I32_RS04370 (I6I32_04370) comGA/cglA/cilD 902978..903919 (-) 942 WP_198465002.1 competence type IV pilus ATPase ComGA Machinery gene
  I6I32_RS04375 (I6I32_04375) - 903980..904345 (-) 366 WP_000286399.1 DUF1033 family protein -
  I6I32_RS04380 (I6I32_04380) nagA 904498..905649 (-) 1152 WP_198465003.1 N-acetylglucosamine-6-phosphate deacetylase -
  I6I32_RS04385 (I6I32_04385) - 905790..907607 (-) 1818 WP_001220886.1 acyltransferase family protein -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 38589.64 Da        Isoelectric Point: 9.5634

>NTDB_id=516653 I6I32_RS04365 WP_198465068.1 902014..903030(-) (comGB/cglB) [Streptococcus oralis strain FDAARGOS_1075]
MDISQVFRLKRKKLATAKQKKIITLFNNLFSSGFHLVEIISFLSRSALLEKDYVAQMHQGLSQGKSFSEMMDNLGFSSAI
VTQLSLAEVHGNLHLSLGKIEEYLDNLSKVKKKLIEVATYPLILLGFLLLIMLGLRNYLLPQLDSSNIATQIIGNLPQIF
LGLVLVCSLSLLLALTFYKRSSKMRVFSMLARVPFLGIFVQTYLTAYYAREWGNMISQGMELTQIFQIMQEQGSQLFKEI
GQDLAQALQNGREFSQTITTYPFFKKELSLIIEYGEVKSKLGSELEIYAEKTWEAFFTRVNRTMNLVQPLVFIFVALIIV
LLYAAMLMPMYQNMEVNF

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=516653 I6I32_RS04365 WP_198465068.1 902014..903030(-) (comGB/cglB) [Streptococcus oralis strain FDAARGOS_1075]
ATGGACATATCACAAGTCTTCAGGCTGAAACGGAAAAAATTAGCTACAGCTAAGCAGAAGAAAATCATCACCTTGTTTAA
CAACCTCTTTTCCAGTGGCTTTCATTTGGTGGAAATTATTTCTTTCTTGAGCAGAAGTGCCCTGCTGGAAAAGGACTATG
TGGCCCAGATGCACCAAGGCTTGTCTCAGGGGAAATCCTTCTCAGAGATGATGGACAACTTGGGCTTTTCAAGTGCTATT
GTGACCCAGTTATCTCTAGCTGAAGTGCATGGAAATCTTCACCTGAGTCTGGGGAAGATAGAAGAATATCTGGATAATTT
GTCCAAGGTCAAGAAGAAGTTAATCGAAGTGGCGACCTATCCCCTGATTTTGCTGGGATTTCTCCTGCTAATCATGCTGG
GGTTGAGAAACTATCTACTTCCCCAACTGGACAGTAGCAATATTGCCACTCAAATCATCGGCAATCTGCCACAAATATTT
CTGGGGCTAGTGCTGGTTTGCTCTCTATCTTTACTTTTAGCCCTCACTTTCTACAAAAGAAGTTCCAAGATGCGGGTTTT
CTCGATGTTGGCACGGGTTCCCTTTCTAGGAATCTTTGTCCAGACCTATCTGACGGCCTATTACGCGCGTGAATGGGGCA
ATATGATTTCACAGGGGATGGAGCTGACGCAAATTTTTCAGATCATGCAGGAACAAGGTTCTCAGCTCTTTAAAGAAATC
GGTCAAGATTTGGCTCAAGCCCTGCAAAATGGCCGCGAATTTTCTCAAACCATAACGACCTATCCTTTCTTTAAGAAGGA
GTTGAGTCTCATCATCGAGTATGGAGAAGTCAAGTCCAAGCTGGGGAGTGAGTTGGAAATCTATGCTGAAAAAACTTGGG
AAGCCTTTTTTACCCGAGTCAACCGCACCATGAATCTGGTGCAGCCACTGGTTTTTATCTTTGTGGCCCTGATTATCGTT
TTACTTTATGCGGCAATGCTTATGCCCATGTATCAAAATATGGAGGTAAATTTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(24-141)

Type II secretion system (T2SS), protein F (T2SSF)

(209-329)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB/cglB Streptococcus mitis SK321

92.899

100

0.929
  comGB/cglB Streptococcus mitis NCTC 12261

92.308

100

0.923
  comGB/cglB Streptococcus pneumoniae Rx1

90.828

100

0.908
  comGB/cglB Streptococcus pneumoniae D39

90.828

100

0.908
  comGB/cglB Streptococcus pneumoniae R6

90.828

100

0.908
  comGB/cglB Streptococcus pneumoniae TIGR4

90.828

100

0.908
  comYB Streptococcus gordonii str. Challis substr. CH1

72.619

99.408

0.722
  comYB Streptococcus mutans UA140

58.934

94.379

0.556
  comYB Streptococcus mutans UA159

58.934

94.379

0.556
  comGB Lactococcus lactis subsp. cremoris KW2

52.071

100

0.521