Detailed information    

insolico Bioinformatically predicted

Overview

Name   priA   Type   Machinery gene
Locus tag   SIR_RS16350 Genome accession   NC_022246
Coordinates   1358577..1360958 (-) Length   793 a.a.
NCBI ID   WP_021003047.1    Uniprot ID   T1ZFD9
Organism   Streptococcus intermedius B196     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1353577..1365958
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SIR_RS16330 (SIR_1294) stkP 1353648..1355522 (-) 1875 WP_003073669.1 Stk1 family PASTA domain-containing Ser/Thr kinase Regulator
  SIR_RS16335 (SIR_1295) - 1355519..1356259 (-) 741 WP_003073671.1 Stp1/IreP family PP2C-type Ser/Thr phosphatase -
  SIR_RS16340 (SIR_1296) rsmB 1356278..1357591 (-) 1314 WP_021003045.1 16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB -
  SIR_RS16345 (SIR_1297) fmt 1357581..1358516 (-) 936 WP_041787505.1 methionyl-tRNA formyltransferase -
  SIR_RS16350 (SIR_1298) priA 1358577..1360958 (-) 2382 WP_021003047.1 primosomal protein N' Machinery gene
  SIR_RS16355 (SIR_1299) rpoZ 1361017..1361331 (-) 315 WP_003028415.1 DNA-directed RNA polymerase subunit omega -
  SIR_RS16360 (SIR_1300) gmk 1361357..1361983 (-) 627 WP_003078308.1 guanylate kinase -
  SIR_RS16365 (SIR_1301) - 1362165..1363772 (-) 1608 WP_003073681.1 ribonuclease Y -
  SIR_RS19730 (SIR_1302) - 1363988..1364461 (+) 474 Protein_1295 S-ribosylhomocysteine lyase -
  SIR_RS16370 (SIR_1303) - 1364518..1365555 (-) 1038 WP_185756690.1 ORF6N domain-containing protein -

Sequence

Protein


Download         Length: 793 a.a.        Molecular weight: 90333.81 Da        Isoelectric Point: 9.1601

>NTDB_id=51128 SIR_RS16350 WP_021003047.1 1358577..1360958(-) (priA) [Streptococcus intermedius B196]
MRIAKVIVDVPLMQTDRPYSYAVPKEFSTMLEIGMRVHVPFGKANRLIQGIVVGLLEERTIGLKEIVEVLDFTPVLNEEQ
LWLAEELRKSVFAYKISILKAMLPGFLNSSYDKILHAQPALTKEDRMLIFGNVDQRHFSSLDKTLQAKVMRLTRQGAIQL
EYQATDQKHIKTEKWYRVHHERLGQLDIPNRAKKKQALRSLLQNQTSLQRLADLKETFSREVIHYFIEQDVLQVIEKEVS
RSETYFENVSATSALVLNEEQKGAVQAITQQIGHTGKPFLLEGVTGSGKTEVYLQVIQEALSRGKTAIMLVPEISLTPQV
TNRFISRFGKKVAILHSGLSNGEKYDEWRKVERGEAQVVVGARSAIFAPLKNIGAIIVDEEHEASYKQDSNPRYHARDVA
LLRAKYNQAVLVLGSATPSLESRARAGKGVYHFLQLTKRANPQAQIPEVEIVDFRDYIGQNEASDFTPVLLEAIKDRLEK
KEQVVLMLNRRGYSSFVMCRECGAVDSCPNCDISLTLHMDTKSMNCHYCGFSKVIPHSCPNCSSRSIRYYGTGTQKAYDE
LAEAFPQARILRMDVDTTRKKGSHEQILKKFEERHADILLGTQMIAKGLDFPNVTLVGVLNADTALNLPDFRSSERTFQL
LTQVAGRAGRAEKAGHVIIQSYNPRHYAIVFAKNQDYEGFYAYEMGVRRQLSYPPYYFTVGITLSHKIEAEVIKKSYEVL
NLIRSGLSNKVQVLGPTPKPIARTHNLYHYQLIIKYRFEEHLQDVLNQVLDWTQEKDNQNLRLIIDKEPQNFM

Nucleotide


Download         Length: 2382 bp        

>NTDB_id=51128 SIR_RS16350 WP_021003047.1 1358577..1360958(-) (priA) [Streptococcus intermedius B196]
ATGCGGATTGCAAAGGTCATTGTAGATGTTCCTCTAATGCAAACGGATAGACCTTATAGTTATGCAGTTCCGAAAGAATT
TTCGACTATGCTAGAAATTGGTATGCGTGTCCATGTGCCTTTTGGGAAAGCTAATCGCCTGATTCAAGGGATTGTTGTAG
GGTTATTAGAAGAAAGGACGATAGGATTAAAAGAAATCGTAGAGGTATTGGACTTTACACCAGTTTTAAATGAGGAGCAA
CTGTGGCTAGCAGAAGAATTGAGAAAATCTGTCTTTGCTTACAAAATTTCCATCTTAAAAGCTATGTTGCCTGGTTTTCT
AAACTCCAGTTATGATAAAATTCTCCATGCTCAGCCCGCTTTGACGAAAGAAGATCGAATGCTTATTTTTGGCAATGTAG
ATCAGCGGCATTTTTCATCGCTTGATAAAACACTCCAAGCTAAGGTAATGCGTCTAACACGGCAAGGGGCGATTCAACTC
GAGTATCAGGCAACTGATCAAAAGCATATAAAAACAGAAAAATGGTATCGAGTCCATCATGAGAGATTAGGTCAGTTAGA
CATTCCTAATCGGGCGAAAAAAAAGCAAGCTTTGCGATCGCTTTTACAGAATCAGACCAGTTTGCAACGTCTAGCAGATC
TGAAAGAAACGTTTTCACGAGAGGTTATTCACTATTTTATAGAACAAGACGTTTTGCAAGTCATTGAAAAAGAGGTGAGT
CGCTCTGAAACATATTTTGAGAATGTTTCAGCGACGTCTGCATTGGTGTTAAATGAAGAGCAAAAAGGGGCTGTTCAAGC
TATTACTCAGCAAATCGGGCACACAGGGAAGCCATTTCTACTAGAAGGAGTAACAGGGAGTGGCAAAACGGAAGTGTATT
TGCAGGTTATTCAAGAAGCACTCAGCCGTGGTAAAACAGCTATTATGTTAGTTCCTGAAATCTCTTTGACACCGCAAGTT
ACTAATCGCTTTATTTCGCGTTTTGGAAAGAAAGTTGCTATTCTTCATTCGGGACTTTCTAACGGTGAGAAATATGATGA
ATGGCGCAAGGTTGAGCGAGGAGAAGCACAAGTTGTTGTTGGTGCGCGTTCTGCTATTTTTGCTCCATTAAAAAACATTG
GTGCGATTATCGTTGATGAGGAGCATGAGGCAAGCTACAAGCAAGACTCCAATCCACGCTATCATGCACGAGATGTTGCC
TTGCTACGTGCAAAGTACAATCAAGCCGTTTTAGTGCTAGGGTCAGCGACCCCGAGTTTAGAAAGTAGAGCGCGAGCTGG
CAAGGGAGTTTATCATTTTTTACAATTAACTAAACGAGCCAATCCGCAAGCTCAGATTCCTGAAGTAGAAATTGTGGATT
TTCGTGATTATATTGGGCAAAACGAAGCTAGTGATTTTACTCCGGTTCTTTTAGAAGCAATTAAAGACCGATTAGAAAAA
AAAGAGCAAGTTGTTTTGATGTTGAACCGACGAGGCTATTCTAGTTTTGTCATGTGTCGTGAGTGTGGTGCGGTTGATAG
TTGCCCAAATTGTGACATTTCTCTGACCTTGCATATGGATACGAAAAGTATGAATTGCCACTATTGTGGTTTTTCAAAGG
TGATTCCTCATAGCTGTCCGAATTGTTCCAGTCGGAGCATTCGTTATTATGGAACGGGGACGCAAAAGGCTTACGATGAA
TTAGCAGAAGCTTTTCCGCAAGCTCGAATTTTAAGAATGGATGTTGATACTACTCGTAAAAAAGGTAGTCACGAACAAAT
CTTGAAAAAATTTGAGGAAAGACATGCTGATATTTTACTGGGAACACAGATGATTGCAAAAGGTCTCGATTTTCCAAATG
TGACTCTTGTAGGGGTGCTAAATGCAGATACAGCGTTAAATTTACCAGATTTTCGTTCATCTGAGCGAACCTTTCAACTT
TTAACTCAGGTTGCAGGACGTGCAGGACGAGCTGAAAAAGCAGGACATGTGATTATTCAAAGTTACAATCCAAGACATTA
TGCGATTGTCTTTGCAAAGAATCAGGATTATGAGGGTTTTTATGCCTATGAAATGGGTGTCAGAAGGCAATTATCCTATC
CACCTTATTACTTTACGGTGGGGATTACTTTATCACACAAAATAGAAGCTGAGGTTATTAAAAAGTCATACGAAGTGTTA
AATCTGATACGTTCTGGCTTATCAAATAAGGTTCAGGTATTGGGCCCAACTCCTAAGCCGATTGCTCGTACTCACAATCT
CTATCATTACCAATTGATTATTAAATATCGTTTTGAAGAGCATTTGCAAGATGTTTTAAATCAGGTTTTAGATTGGACAC
AAGAAAAGGACAATCAAAACTTACGTTTAATTATAGACAAGGAACCACAAAATTTTATGTGA

Domains

Predicted by InterProScan.

Primosomal protein N C-terminal domain (PriA_C)

(695-789)

Type III restriction enzyme, res subunit (ResIII)

(256-419)

3DNA-binding domain (3BD) (PriA_3primeBD)

(5-104)

Helicase conserved C-terminal domain (Helicase_C)

(563-651)

PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA)

(508-533)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB T1ZFD9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  priA Bacillus subtilis subsp. subtilis str. 168

46.173

100

0.472

Multiple sequence alignment