Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA/celA/cilE   Type   Machinery gene
Locus tag   SPAF_RS03705 Genome accession   NC_017905
Coordinates   719436..720104 (+) Length   222 a.a.
NCBI ID   WP_014713070.1    Uniprot ID   I1ZKY6
Organism   Streptococcus parasanguinis FW213     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 714436..725104
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SPAF_RS03685 (Spaf_0700) - 715023..715292 (-) 270 WP_013903293.1 GIY-YIG nuclease family protein -
  SPAF_RS03690 (Spaf_0701) - 715282..716028 (-) 747 WP_003017755.1 tRNA1(Val) (adenine(37)-N6)-methyltransferase -
  SPAF_RS03695 (Spaf_0702) - 716109..718448 (-) 2340 WP_041826264.1 cation-translocating P-type ATPase -
  SPAF_RS03700 (Spaf_0703) - 718623..719369 (+) 747 WP_014713069.1 1-acyl-sn-glycerol-3-phosphate acyltransferase -
  SPAF_RS03705 (Spaf_0704) comEA/celA/cilE 719436..720104 (+) 669 WP_014713070.1 helix-hairpin-helix domain-containing protein Machinery gene
  SPAF_RS03710 (Spaf_0705) comEC/celB 720085..722331 (+) 2247 WP_014713071.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  SPAF_RS03715 (Spaf_0706) - 722407..723009 (+) 603 WP_014713072.1 DUF805 domain-containing protein -
  SPAF_RS03720 (Spaf_0707) holA 723033..724067 (+) 1035 WP_014713073.1 DNA polymerase III subunit delta -
  SPAF_RS03725 (Spaf_0708) sodA 724155..724760 (+) 606 WP_003017685.1 superoxide dismutase SodA -

Sequence

Protein


Download         Length: 222 a.a.        Molecular weight: 23813.73 Da        Isoelectric Point: 5.7287

>NTDB_id=51128 SPAF_RS03705 WP_014713070.1 719436..720104(+) (comEA/celA/cilE) [Streptococcus parasanguinis FW213]
MEDIIEKIKEYKLFLALTAIGLLIGGYFLFHRPQSSASTIPDLYQASSSTSSKEKVQTTSSKEEKTVTSVSAAPEIITVD
VKGAVKQPGVYELRSNSRVHDAIHKAGGMTVDANSQSVNLAQKLSDEAVIYVAKEGEDVPALGSSESPATSSAPAEKTGK
VHLNRATETELQTVSGIGQKRAQDIIAYREANGPFRSVDDLKNVSGIGEKTLEKLRDAFTVD

Nucleotide


Download         Length: 669 bp        

>NTDB_id=51128 SPAF_RS03705 WP_014713070.1 719436..720104(+) (comEA/celA/cilE) [Streptococcus parasanguinis FW213]
ATGGAGGATATCATCGAAAAAATCAAAGAATATAAGCTCTTTTTAGCTCTTACTGCTATTGGACTCTTAATTGGAGGGTA
TTTTCTTTTCCATCGACCTCAGTCAAGTGCATCAACCATACCGGATCTTTATCAAGCTTCTAGTTCAACTTCGAGCAAAG
AAAAGGTGCAGACCACTAGTTCCAAAGAAGAAAAGACGGTTACTTCAGTGTCTGCTGCACCCGAGATCATTACGGTCGAT
GTCAAAGGAGCTGTCAAACAACCAGGTGTCTATGAGCTACGCTCAAATAGCAGAGTCCACGATGCCATTCATAAGGCGGG
TGGGATGACAGTAGATGCCAATAGCCAATCGGTCAACTTGGCGCAAAAACTCTCTGATGAAGCAGTGATCTATGTTGCTA
AAGAAGGAGAGGATGTTCCAGCACTTGGAAGTTCAGAAAGTCCTGCAACTTCGTCAGCGCCAGCAGAAAAAACAGGCAAG
GTCCATTTAAATCGAGCGACAGAGACTGAGCTTCAAACGGTATCGGGCATTGGACAGAAACGTGCCCAGGACATTATCGC
CTATCGCGAAGCAAATGGTCCTTTTCGATCAGTGGATGATCTGAAGAATGTTTCAGGAATCGGAGAGAAAACACTGGAGA
AACTAAGAGATGCTTTCACGGTGGATTAA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(158-219)

SLBB domain (SLBB)

(79-132)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB I1ZKY6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA/celA/cilE Streptococcus mitis NCTC 12261

51.351

100

0.514
  comEA/celA/cilE Streptococcus mitis SK321

50

100

0.505
  comEA/celA/cilE Streptococcus pneumoniae TIGR4

50

100

0.5
  comEA/celA/cilE Streptococcus pneumoniae D39

49.55

100

0.496
  comEA/celA/cilE Streptococcus pneumoniae Rx1

49.55

100

0.496
  comEA/celA/cilE Streptococcus pneumoniae R6

49.55

100

0.496
  comEA Streptococcus thermophilus LMD-9

43.421

100

0.446
  comEA Lactococcus lactis subsp. cremoris KW2

41.88

100

0.441
  comEA Staphylococcus aureus MW2

36.323

100

0.365

Multiple sequence alignment