Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   IT895_RS09570 Genome accession   NZ_CP065230
Coordinates   2041307..2042539 (+) Length   410 a.a.
NCBI ID   WP_085918493.1    Uniprot ID   A0A7T0RHY2
Organism   Halomonas sp. A40-4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2036307..2047539
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IT895_RS09545 (IT895_09570) - 2036919..2037737 (-) 819 WP_199285353.1 inositol monophosphatase family protein -
  IT895_RS09550 (IT895_09575) - 2037763..2038209 (-) 447 WP_199285445.1 anti-virulence regulator CigR family protein -
  IT895_RS09555 (IT895_09580) - 2038300..2038755 (-) 456 WP_199285354.1 pilin -
  IT895_RS19350 (IT895_09585) - 2038838..2039305 (-) 468 WP_186255736.1 pilin -
  IT895_RS09565 (IT895_09590) pilB 2039546..2041303 (+) 1758 WP_199285355.1 type IV-A pilus assembly ATPase PilB Machinery gene
  IT895_RS09570 (IT895_09595) pilC 2041307..2042539 (+) 1233 WP_085918493.1 type II secretion system F family protein Machinery gene
  IT895_RS09575 (IT895_09600) - 2042561..2043406 (+) 846 WP_085918492.1 A24 family peptidase -
  IT895_RS09580 (IT895_09605) coaE 2043446..2044048 (+) 603 WP_199285356.1 dephospho-CoA kinase -
  IT895_RS09585 (IT895_09610) yacG 2044111..2044344 (+) 234 WP_199285357.1 DNA gyrase inhibitor YacG -
  IT895_RS09590 (IT895_09615) - 2044345..2045220 (+) 876 WP_199285358.1 DNA replication terminus site-binding protein -
  IT895_RS09595 (IT895_09620) - 2045258..2045584 (-) 327 WP_199285359.1 hypothetical protein -
  IT895_RS09600 (IT895_09625) - 2045957..2046787 (+) 831 WP_199285360.1 helix-turn-helix transcriptional regulator -
  IT895_RS09605 (IT895_09630) - 2046857..2047231 (-) 375 WP_085918486.1 histone-like nucleoid-structuring protein, MvaT/MvaU family -

Sequence

Protein


Download         Length: 410 a.a.        Molecular weight: 44940.47 Da        Isoelectric Point: 10.4904

>NTDB_id=508254 IT895_RS09570 WP_085918493.1 2041307..2042539(+) (pilC) [Halomonas sp. A40-4]
MAKSPRARRQPGIQLYRWKWEGTGPQNRTLSGEIIGRSKADVASELANQQINVKRLRKKGGLSGRGRISPHDIMVFARQM
ATMIRAGIPLLQAFQVVAESLKKPAMVALVQQMMSDVSAGSSFSDALRRHPRHFDRLFVNLVEAGEQSGALDQMLDRIAT
YKEKVESLKSRVKKAMWYPSAVLLIGVGVTLLLLIKVVPEFDSMFDSFGAELPALTQMTVNLSSLAQRYWLYALGGVVAG
ILLLKQAINRSPRVAYRAHSVLLRLPVVGDILHKSAVARFARTLATTFASGVPLVEGLDTASGATGNRVYERAVTRTRHD
VATGQQLHFAMRMTNQFPPLAVQMVSIGEEAGSLDAMLNRVADYYEEEVDNKVDALTSLMEPLIIVVLGLLVGGVVVSMY
LPIFNLGSAL

Nucleotide


Download         Length: 1233 bp        

>NTDB_id=508254 IT895_RS09570 WP_085918493.1 2041307..2042539(+) (pilC) [Halomonas sp. A40-4]
ATGGCCAAGTCGCCTCGGGCTCGTCGTCAGCCGGGTATTCAGCTTTACCGCTGGAAGTGGGAAGGCACTGGGCCCCAGAA
CCGCACGCTTAGCGGTGAAATAATTGGTCGCAGCAAGGCAGACGTCGCCAGCGAGCTCGCCAATCAGCAGATCAACGTAA
AGCGGTTACGCAAGAAAGGCGGCCTCAGCGGTCGTGGTCGAATCAGTCCGCACGATATCATGGTGTTTGCCCGCCAGATG
GCGACCATGATTCGGGCAGGCATTCCGCTACTTCAGGCGTTTCAGGTGGTCGCCGAGAGCCTCAAAAAACCGGCAATGGT
GGCGCTGGTCCAGCAAATGATGAGCGATGTATCCGCTGGTTCCAGCTTCTCCGATGCACTGCGCCGCCACCCCAGGCACT
TTGACCGCCTGTTCGTGAACCTTGTTGAAGCAGGCGAGCAGTCCGGGGCACTTGACCAGATGCTTGACCGCATTGCCACT
TACAAGGAGAAAGTCGAGTCGCTTAAAAGCCGCGTCAAGAAGGCCATGTGGTACCCCTCCGCGGTCCTCCTGATCGGTGT
CGGCGTCACCCTTCTTCTGCTGATCAAGGTGGTGCCCGAATTTGACAGCATGTTTGACAGCTTTGGCGCAGAACTCCCCG
CCTTGACGCAGATGACGGTCAACCTATCGAGCCTGGCCCAGCGTTACTGGCTATATGCATTGGGGGGCGTCGTCGCGGGG
ATCTTGCTGCTCAAACAGGCGATTAACCGCTCTCCCCGCGTTGCTTACCGCGCGCACAGTGTCCTGTTACGCCTGCCCGT
CGTCGGTGACATCCTGCATAAATCGGCAGTGGCACGATTTGCCCGGACCCTGGCGACCACCTTTGCGTCTGGCGTGCCGT
TGGTTGAAGGATTGGACACGGCGTCCGGGGCCACCGGCAATAGAGTCTATGAACGCGCCGTCACCCGGACGCGCCATGAC
GTGGCCACCGGTCAGCAGTTACACTTCGCCATGCGCATGACCAATCAGTTTCCGCCCCTGGCAGTGCAGATGGTCAGCAT
CGGTGAAGAAGCAGGCTCGCTGGATGCCATGCTCAACCGCGTCGCGGACTACTACGAAGAAGAAGTCGACAATAAGGTAG
ATGCCTTAACCTCGCTGATGGAACCGCTCATTATCGTGGTCTTGGGCCTGCTGGTCGGCGGTGTTGTGGTGTCGATGTAC
TTACCCATCTTTAACTTAGGCTCGGCCCTGTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(76-199)

Type II secretion system (T2SS), protein F (T2SSF)

(280-402)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7T0RHY2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

52.12

97.805

0.51
  pilC Acinetobacter baylyi ADP1

50

97.561

0.488
  pilC Acinetobacter baumannii D1279779

49.874

96.829

0.483
  pilC Legionella pneumophila strain ERS1305867

48.507

98.049

0.476
  pilC Vibrio cholerae strain A1552

43.75

97.561

0.427
  pilC Vibrio campbellii strain DS40M4

43.392

97.805

0.424
  pilG Neisseria gonorrhoeae MS11

42.394

97.805

0.415
  pilG Neisseria meningitidis 44/76-A

42.145

97.805

0.412
  pilC Thermus thermophilus HB27

36.386

100

0.368