Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   IT895_RS09565 Genome accession   NZ_CP065230
Coordinates   2039546..2041303 (+) Length   585 a.a.
NCBI ID   WP_199285355.1    Uniprot ID   A0A7T0RHZ9
Organism   Halomonas sp. A40-4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2034546..2046303
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IT895_RS09545 (IT895_09570) - 2036919..2037737 (-) 819 WP_199285353.1 inositol monophosphatase family protein -
  IT895_RS09550 (IT895_09575) - 2037763..2038209 (-) 447 WP_199285445.1 anti-virulence regulator CigR family protein -
  IT895_RS09555 (IT895_09580) - 2038300..2038755 (-) 456 WP_199285354.1 pilin -
  IT895_RS19350 (IT895_09585) - 2038838..2039305 (-) 468 WP_186255736.1 pilin -
  IT895_RS09565 (IT895_09590) pilB 2039546..2041303 (+) 1758 WP_199285355.1 type IV-A pilus assembly ATPase PilB Machinery gene
  IT895_RS09570 (IT895_09595) pilC 2041307..2042539 (+) 1233 WP_085918493.1 type II secretion system F family protein Machinery gene
  IT895_RS09575 (IT895_09600) - 2042561..2043406 (+) 846 WP_085918492.1 A24 family peptidase -
  IT895_RS09580 (IT895_09605) coaE 2043446..2044048 (+) 603 WP_199285356.1 dephospho-CoA kinase -
  IT895_RS09585 (IT895_09610) yacG 2044111..2044344 (+) 234 WP_199285357.1 DNA gyrase inhibitor YacG -
  IT895_RS09590 (IT895_09615) - 2044345..2045220 (+) 876 WP_199285358.1 DNA replication terminus site-binding protein -
  IT895_RS09595 (IT895_09620) - 2045258..2045584 (-) 327 WP_199285359.1 hypothetical protein -

Sequence

Protein


Download         Length: 585 a.a.        Molecular weight: 64221.50 Da        Isoelectric Point: 5.9693

>NTDB_id=508253 IT895_RS09565 WP_199285355.1 2039546..2041303(+) (pilB) [Halomonas sp. A40-4]
MSEPYFESTLSHAAARGGLRGIAQRLVKHGLLTEAQAATAEATAFDLDISLLQHVVDSGLVDPRQSAIAAGWEYGLPVID
LNAVRLSSLPPAAEYPVKVLQNLTVLPLARHGHRLTVAVPYPATLTQLDELQFATGLSVEGVLAPVDQLSTALNQYLTQN
ERSMLEELDDIDDAVSSLNVVQGAAGEDAPLEEAPQNSDDAPIVKFVNKLLLDAIRRGASDIHFEPYETQYRVRFRIDGM
LIEVARPPFAMRNRIAARLKIMARLDISERRLPQDGAIKLKLSRTRSLDFRVNSLPTVYGEKLVLRILDPTATQLGIEQL
GFTDDQRGYYERALAQPQGMILVTGPTGSGKTVSLYTGINILNQIERNICTAEDPVEIKVPGVNQVNVLPKIGLDFASAL
RAFLRQDPDVVMVGEIRDLETAEISIKAAQTGHLVLSTVHTNSAAETLTRLSNMGVSSFNIASAVSLIIAQRLARKLCPH
CKEPADIPHETLRREGFSQQEIQQATLFRPVGCKQCTHGYKGRVGVYEVVPITDTMRQLVMRDANSLDIDQLARREGYPS
LHQSGLLKVMQGITSLEEVNRISKE

Nucleotide


Download         Length: 1758 bp        

>NTDB_id=508253 IT895_RS09565 WP_199285355.1 2039546..2041303(+) (pilB) [Halomonas sp. A40-4]
ATGAGCGAACCCTATTTCGAGTCAACCTTAAGCCATGCCGCCGCCCGGGGCGGACTGCGTGGTATCGCCCAACGCCTGGT
CAAGCACGGGTTGCTGACCGAGGCTCAGGCGGCCACCGCTGAAGCGACAGCGTTCGACTTGGATATCTCACTGCTCCAGC
ATGTCGTTGACAGCGGCCTGGTCGATCCCAGGCAATCAGCCATTGCCGCTGGCTGGGAGTATGGGTTGCCGGTGATTGAT
CTCAACGCCGTACGGTTATCGTCACTGCCACCAGCGGCTGAGTACCCTGTTAAAGTCCTTCAGAACTTGACGGTGCTGCC
ATTGGCACGCCACGGTCACCGGCTCACTGTTGCCGTCCCTTACCCGGCGACGCTTACCCAACTGGATGAGTTGCAATTTG
CCACCGGGCTAAGCGTTGAAGGTGTTCTCGCGCCTGTTGATCAGTTGAGCACGGCGTTAAACCAGTATCTCACCCAGAAT
GAGCGCAGCATGCTGGAGGAGCTTGACGACATCGACGATGCCGTCAGTTCCTTGAATGTCGTCCAGGGAGCCGCCGGTGA
AGACGCTCCCCTTGAGGAAGCACCGCAGAATAGCGACGACGCACCGATCGTCAAATTCGTCAATAAACTGCTGCTGGATG
CGATTCGCCGGGGCGCCTCAGATATCCACTTTGAACCCTATGAAACCCAGTACCGGGTTCGCTTCCGGATTGATGGCATG
CTGATCGAGGTTGCACGGCCGCCTTTCGCCATGCGTAACCGAATCGCCGCGCGCTTGAAGATCATGGCCCGGCTGGATAT
CTCCGAGCGCCGCTTGCCCCAGGACGGTGCGATCAAACTCAAGCTTTCACGCACTCGCTCCCTCGACTTCAGGGTCAATT
CACTGCCGACTGTGTATGGCGAGAAGCTCGTACTGAGGATCCTTGACCCCACCGCCACGCAATTGGGCATCGAGCAGTTA
GGCTTCACCGATGACCAGCGCGGTTACTACGAGCGGGCGCTGGCGCAGCCGCAAGGCATGATTCTGGTGACCGGCCCCAC
GGGCAGTGGTAAAACCGTATCGCTGTATACCGGGATCAATATCCTTAACCAGATAGAGCGCAATATCTGCACTGCCGAAG
ACCCAGTGGAAATCAAGGTCCCTGGCGTTAACCAGGTCAACGTACTGCCAAAAATCGGTCTGGATTTCGCCAGCGCGTTA
AGGGCATTCCTGCGCCAGGATCCTGACGTGGTCATGGTCGGTGAAATTCGCGACCTGGAAACCGCCGAGATCTCCATCAA
GGCGGCCCAGACCGGCCACCTAGTGCTGTCAACGGTACATACCAACTCGGCGGCCGAAACCCTCACTCGCCTGTCCAATA
TGGGGGTGTCTTCCTTCAATATTGCCAGCGCGGTCAGTTTGATCATTGCTCAGCGCCTGGCGCGCAAGCTTTGCCCTCAC
TGCAAGGAACCGGCTGATATTCCCCACGAAACACTAAGACGCGAAGGGTTCAGCCAACAGGAGATTCAGCAGGCTACCCT
ATTTCGCCCGGTCGGCTGCAAGCAGTGTACCCATGGCTACAAGGGGCGTGTGGGTGTCTACGAAGTCGTGCCGATAACCG
ATACGATGCGCCAGTTGGTCATGCGCGATGCCAACTCTCTGGATATTGACCAGTTGGCCCGCCGTGAGGGGTATCCGAGT
CTGCATCAAAGTGGTCTTTTAAAGGTGATGCAAGGGATCACGAGCCTTGAGGAAGTTAACCGTATCAGCAAGGAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(209-476)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(26-159)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7T0RHZ9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

53.18

96.752

0.515
  pilB Acinetobacter baumannii D1279779

51.323

96.923

0.497
  pilB Acinetobacter baylyi ADP1

50

98.12

0.491
  pilB Vibrio campbellii strain DS40M4

48.592

97.094

0.472
  pilB Vibrio parahaemolyticus RIMD 2210633

48.929

95.726

0.468
  pilB Vibrio cholerae strain A1552

51.272

87.35

0.448
  pilF Neisseria gonorrhoeae MS11

49.791

81.709

0.407