Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA/cglA   Type   Machinery gene
Locus tag   I2435_RS00720 Genome accession   NZ_CP065059
Coordinates   122106..123044 (+) Length   312 a.a.
NCBI ID   WP_206156531.1    Uniprot ID   -
Organism   Streptococcus equi subsp. zooepidemicus strain SEZ25     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 117106..128044
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I2435_RS00700 (JFMEOBDD_00141) - 117471..118703 (+) 1233 WP_014622136.1 hypothetical protein -
  I2435_RS10110 - 119095..119211 (+) 117 WP_231145532.1 hypothetical protein -
  I2435_RS00710 (JFMEOBDD_00142) - 119276..121279 (+) 2004 WP_206156530.1 DUF1430 domain-containing protein -
  I2435_RS00715 (JFMEOBDD_00143) - 121281..121925 (+) 645 WP_111677648.1 ATP-binding cassette domain-containing protein -
  I2435_RS00720 (JFMEOBDD_00144) comGA/cglA 122106..123044 (+) 939 WP_206156531.1 competence type IV pilus ATPase ComGA Machinery gene
  I2435_RS00725 (JFMEOBDD_00145) comGB 122977..124010 (+) 1034 Protein_110 competence type IV pilus assembly protein ComGB -
  I2435_RS00730 (JFMEOBDD_00146) comGC/cglC 124011..124334 (+) 324 WP_012677235.1 competence type IV pilus major pilin ComGC Machinery gene
  I2435_RS00735 (JFMEOBDD_00147) comGD 124312..124737 (+) 426 WP_012514783.1 competence type IV pilus minor pilin ComGD -
  I2435_RS00740 comGE 124694..124984 (+) 291 WP_043030351.1 competence type IV pilus minor pilin ComGE -
  I2435_RS00745 (JFMEOBDD_00148) comYF 124971..125405 (+) 435 WP_206156890.1 competence type IV pilus minor pilin ComGF Machinery gene
  I2435_RS00750 (JFMEOBDD_00149) comGG 125383..125745 (+) 363 WP_206156532.1 competence type IV pilus minor pilin ComGG -
  I2435_RS00755 - 125812..127154 (+) 1343 WP_206156533.1 IS3 family transposase -

Sequence

Protein


Download         Length: 312 a.a.        Molecular weight: 35369.58 Da        Isoelectric Point: 7.1483

>NTDB_id=506460 I2435_RS00720 WP_206156531.1 122106..123044(+) (comGA/cglA) [Streptococcus equi subsp. zooepidemicus strain SEZ25]
MVQELAKRLIRQAEELQAQDIYILPRGASYELLMRIGDDRRLIDVCESDRMANLISHFKFVAGMNVGEKRRYQLGACDYD
LDGAKVSLRLSAVGDYQGQESLVIRLLYHHQRQLRYWFDGLERVTSAIGARGLYLFSGPVGSGKTTLMYQLVSDYCKELQ
VISIEDPVEIKNDQLLQLQVNDSIGMTYDNLIKLSLRHRPDVLIIGEIRDTQTARAVIRASLTGAMVFSTVHAKSISGVY
ARLLELGISRAELDNSLAMVAYQRFISGGALIDCAQKEFEHHQANRWNQQIDQLLAEGHLNTRQARLEKIIQ

Nucleotide


Download         Length: 939 bp        

>NTDB_id=506460 I2435_RS00720 WP_206156531.1 122106..123044(+) (comGA/cglA) [Streptococcus equi subsp. zooepidemicus strain SEZ25]
ATGGTTCAAGAATTAGCCAAGAGGCTTATCAGGCAGGCCGAAGAATTGCAGGCTCAGGATATTTATATACTGCCAAGAGG
GGCAAGCTATGAGCTTTTGATGAGGATAGGTGATGACAGGAGGCTAATAGATGTTTGTGAAAGTGATCGGATGGCTAATC
TTATTAGTCACTTTAAGTTCGTTGCAGGAATGAATGTGGGAGAAAAAAGACGCTACCAGCTCGGTGCTTGTGACTACGAC
CTTGATGGCGCTAAGGTATCTCTACGATTATCAGCTGTGGGTGACTATCAGGGACAGGAGAGCTTGGTTATTCGTCTCTT
GTATCATCATCAACGCCAGCTCAGGTATTGGTTTGATGGTTTAGAGCGAGTCACATCAGCTATTGGAGCCAGAGGCCTTT
ACCTCTTTTCTGGACCGGTTGGATCAGGTAAGACAACCCTGATGTATCAGCTAGTCTCTGACTATTGCAAGGAGCTTCAG
GTGATCAGTATTGAGGATCCTGTGGAAATTAAAAATGATCAGCTCCTGCAATTACAGGTTAACGACAGCATTGGCATGAC
ATACGATAATCTGATTAAGCTTTCCTTGCGTCATCGTCCAGATGTGTTGATTATCGGAGAAATTAGAGATACACAGACTG
CTAGAGCGGTTATTAGGGCTAGCCTAACAGGAGCAATGGTCTTTTCAACCGTCCATGCAAAGAGTATTTCAGGCGTTTAC
GCTAGGCTGCTAGAATTAGGCATTTCTAGGGCAGAATTGGATAATAGTCTAGCAATGGTTGCTTACCAGCGATTTATTAG
TGGAGGTGCTTTAATTGATTGTGCACAAAAGGAATTTGAACATCATCAAGCCAACCGGTGGAATCAGCAGATTGATCAGC
TTCTTGCAGAGGGACATCTCAATACCAGGCAGGCAAGGCTTGAAAAAATTATCCAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-264)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

68.71

99.359

0.683
  comYA Streptococcus mutans UA140

63.987

99.679

0.638
  comYA Streptococcus mutans UA159

63.987

99.679

0.638
  comYA Streptococcus gordonii str. Challis substr. CH1

61.613

99.359

0.612
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

61.613

99.359

0.612
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

61.29

99.359

0.609
  comGA/cglA/cilD Streptococcus pneumoniae D39

61.29

99.359

0.609
  comGA/cglA/cilD Streptococcus pneumoniae R6

61.29

99.359

0.609
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

61.29

99.359

0.609
  comGA Lactococcus lactis subsp. cremoris KW2

53.055

99.679

0.529
  comGA Latilactobacillus sakei subsp. sakei 23K

40.493

91.026

0.369