Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   Azoinq_RS04605 Genome accession   NZ_CP064782
Coordinates   1032695..1033933 (+) Length   412 a.a.
NCBI ID   WP_216131798.1    Uniprot ID   A0A975XVK1
Organism   Azospira inquinata strain Azo-3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1027695..1038933
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Azoinq_RS04585 (Azoinq_04585) - 1027884..1028486 (+) 603 WP_216131809.1 transglycosylase SLT domain-containing protein -
  Azoinq_RS04590 (Azoinq_04590) ffh 1028538..1029893 (-) 1356 WP_216131806.1 signal recognition particle protein -
  Azoinq_RS04595 (Azoinq_04595) - 1029924..1030766 (+) 843 WP_216131803.1 inner membrane protein YpjD -
  Azoinq_RS04600 (Azoinq_04600) pilB 1030946..1032661 (+) 1716 WP_216131801.1 type IV-A pilus assembly ATPase PilB Machinery gene
  Azoinq_RS04605 (Azoinq_04605) pilC 1032695..1033933 (+) 1239 WP_216131798.1 type II secretion system F family protein Machinery gene
  Azoinq_RS04610 (Azoinq_04610) pilD 1033957..1034814 (+) 858 WP_216131795.1 A24 family peptidase Machinery gene
  Azoinq_RS04615 (Azoinq_04615) - 1034913..1035161 (+) 249 WP_216131792.1 FmdB family zinc ribbon protein -
  Azoinq_RS04620 (Azoinq_04620) - 1035161..1035784 (+) 624 WP_216132211.1 DUF502 domain-containing protein -
  Azoinq_RS04625 (Azoinq_04625) aspS 1035807..1037621 (+) 1815 WP_216131789.1 aspartate--tRNA ligase -
  Azoinq_RS04630 (Azoinq_04630) - 1037621..1038010 (+) 390 WP_216131786.1 ribonuclease domain-containing protein -
  Azoinq_RS04635 (Azoinq_04635) - 1038024..1038437 (+) 414 WP_216131782.1 barstar family protein -
  Azoinq_RS04640 (Azoinq_04640) nudB 1038442..1038891 (+) 450 WP_216131780.1 dihydroneopterin triphosphate diphosphatase -

Sequence

Protein


Download         Length: 412 a.a.        Molecular weight: 44745.84 Da        Isoelectric Point: 10.1199

>NTDB_id=504718 Azoinq_RS04605 WP_216131798.1 1032695..1033933(+) (pilC) [Azospira inquinata strain Azo-3]
MNSAAKKPIKKIDSGLKESVFLWEGKDKHGKLVRGEMRSTSVTVVQATLRRQGIIVAKVSKQRFRSGRKITEKDITLFTR
QLATMMRSGVPLLQAFDIVGKGHANPSVSKLLSDIKSDVETGSSLNQAFKKYPLYFDSLFCNLVAAGEQAGILDSILDRL
AVYKEKILAIKSKIKSALFYPTAVLAVAFIITAIIMIFVIPAFKKVFSDFGADLPAPTVFVIAISDFFVAYWPLILIALG
GGIYGALQAWKRSEKVQMFMDRALLRAPIFGEIIRKSTIARWTRTLATMFAAGVPLVEALDSVGGASGNYVYKVATAKIQ
SEVSTGTSLTAAMQNASIFPNMVVQMVAIGEESGALDAMLSKVADFFEQEVDDSVEALSSLMEPIIMVVLGTLIGGMVIA
MYLPIFKLGSVV

Nucleotide


Download         Length: 1239 bp        

>NTDB_id=504718 Azoinq_RS04605 WP_216131798.1 1032695..1033933(+) (pilC) [Azospira inquinata strain Azo-3]
ATGAACAGCGCCGCAAAAAAACCGATCAAGAAAATTGATAGCGGCTTAAAAGAAAGTGTTTTTCTTTGGGAGGGGAAAGA
CAAACACGGCAAATTGGTACGGGGGGAAATGCGCAGCACCAGCGTTACCGTGGTGCAAGCCACCCTGCGCAGGCAAGGTA
TTATCGTCGCAAAAGTATCTAAGCAACGATTTCGTTCTGGAAGAAAAATCACGGAGAAGGATATTACCCTTTTCACTCGG
CAACTTGCTACCATGATGAGATCCGGCGTCCCGTTATTACAGGCATTTGATATTGTTGGAAAGGGGCACGCCAACCCCTC
CGTATCAAAACTTTTATCAGATATTAAAAGTGATGTCGAAACAGGTAGCTCGCTTAATCAAGCCTTTAAAAAATACCCTC
TATATTTTGATAGCCTATTTTGTAACTTGGTTGCAGCCGGTGAGCAGGCCGGCATATTAGACAGCATACTAGACCGCCTT
GCGGTTTATAAGGAGAAAATCCTTGCAATAAAAAGCAAAATTAAATCTGCCTTATTCTACCCAACGGCAGTGCTTGCTGT
AGCTTTCATTATCACAGCAATTATTATGATTTTTGTAATTCCAGCCTTTAAAAAGGTTTTTAGCGACTTTGGCGCTGACT
TACCTGCACCAACTGTTTTTGTAATAGCAATTTCTGATTTTTTCGTCGCTTATTGGCCGCTTATATTAATTGCCTTGGGC
GGTGGCATATACGGCGCATTACAGGCATGGAAGCGCTCAGAAAAAGTGCAAATGTTTATGGACCGAGCGCTGCTAAGAGC
ACCGATATTCGGAGAGATTATCCGTAAATCCACTATCGCCCGTTGGACCAGAACCCTCGCCACGATGTTTGCTGCGGGAG
TACCGCTGGTCGAAGCGTTGGATTCGGTAGGAGGCGCCTCGGGGAATTACGTATATAAAGTCGCCACCGCAAAGATTCAG
AGTGAGGTCAGTACGGGCACCAGCCTGACCGCCGCTATGCAAAACGCATCCATATTTCCCAATATGGTGGTACAGATGGT
TGCAATTGGGGAAGAATCCGGGGCTTTGGACGCCATGCTCTCAAAGGTCGCAGACTTCTTTGAACAGGAGGTAGACGACT
CGGTAGAGGCTCTCTCCAGTTTAATGGAACCGATCATTATGGTGGTGCTGGGTACGCTGATTGGCGGTATGGTTATCGCC
ATGTATCTTCCTATCTTCAAACTCGGCTCTGTGGTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(78-201)

Type II secretion system (T2SS), protein F (T2SSF)

(282-404)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

54.5

97.087

0.529
  pilC Legionella pneumophila strain ERS1305867

54.798

96.117

0.527
  pilG Neisseria gonorrhoeae MS11

53.149

96.359

0.512
  pilG Neisseria meningitidis 44/76-A

52.897

96.359

0.51
  pilC Acinetobacter baylyi ADP1

50

96.602

0.483
  pilC Acinetobacter baumannii D1279779

49.624

96.845

0.481
  pilC Vibrio cholerae strain A1552

40.201

96.602

0.388
  pilC Vibrio campbellii strain DS40M4

38.235

99.029

0.379
  pilC Thermus thermophilus HB27

36.779

100

0.371