Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   IWH25_RS07275 Genome accession   NZ_CP064781
Coordinates   1474441..1475673 (-) Length   410 a.a.
NCBI ID   WP_203388656.1    Uniprot ID   A0A974SRQ0
Organism   Azospira restricta strain DSM 18626     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1469441..1480673
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IWH25_RS07240 (IWH25_07235) nudB 1469471..1469914 (-) 444 WP_203388650.1 dihydroneopterin triphosphate diphosphatase -
  IWH25_RS07245 (IWH25_07240) - 1469911..1470366 (-) 456 WP_203388651.1 barstar family protein -
  IWH25_RS07250 (IWH25_07245) - 1470377..1470754 (-) 378 WP_203388652.1 ribonuclease domain-containing protein -
  IWH25_RS07255 (IWH25_07250) aspS 1470758..1472572 (-) 1815 WP_203388653.1 aspartate--tRNA ligase -
  IWH25_RS07260 (IWH25_07255) - 1472599..1473213 (-) 615 WP_203389179.1 DUF502 domain-containing protein -
  IWH25_RS07265 (IWH25_07260) - 1473213..1473479 (-) 267 WP_203388654.1 FmdB family zinc ribbon protein -
  IWH25_RS07270 (IWH25_07265) pilD 1473593..1474444 (-) 852 WP_203388655.1 prepilin peptidase Machinery gene
  IWH25_RS07275 (IWH25_07270) pilC 1474441..1475673 (-) 1233 WP_203388656.1 type II secretion system F family protein Machinery gene
  IWH25_RS07280 (IWH25_07275) pilB 1475697..1477412 (-) 1716 WP_203388657.1 type IV-A pilus assembly ATPase PilB Machinery gene
  IWH25_RS07285 (IWH25_07280) - 1477562..1478401 (-) 840 WP_203388658.1 cytochrome C assembly family protein -
  IWH25_RS07290 (IWH25_07285) ffh 1478432..1479793 (+) 1362 WP_203388659.1 signal recognition particle protein -
  IWH25_RS07295 (IWH25_07290) - 1479805..1480410 (-) 606 WP_203388660.1 lytic transglycosylase domain-containing protein -

Sequence

Protein


Download         Length: 410 a.a.        Molecular weight: 44640.50 Da        Isoelectric Point: 9.8519

>NTDB_id=504701 IWH25_RS07275 WP_203388656.1 1474441..1475673(-) (pilC) [Azospira restricta strain DSM 18626]
MATAKPAKKPAGAKEFTFLWEGKNKDGKDVRGELRATSEAVAQATLRRQGVRITKISKQRFKTGKKITDKDITLFTRQLA
TMMKAGVPLLQAFDIVGRGHSNPSVGKLLLDIKADVETGSSLSQAFRKYPLHFDALFCNLVAAGEQAGILEALLDRLATY
KEKMQALKAKIKSALFYPMAILIMAFVITAVIMIFVIPAFKEVFKSFGADLPAPTVMVIAMSDYFVEYWWAIFGSIGGGI
YAFIQAYKRSEAMQIAFDRYALRIPVFGDVIRKSTVARWTRTLATMFAAGVPLVESLDSVGGASGNYVYKVATRQIQSEV
STGTNLTTAMQNSKLFDNMVIQMVAIGEESGALDSMLEKVAGFLEAEVDDAVEALSSLMEPMIMAVLGVLIGGLVIAMYL
PIFKLGSVVG

Nucleotide


Download         Length: 1233 bp        

>NTDB_id=504701 IWH25_RS07275 WP_203388656.1 1474441..1475673(-) (pilC) [Azospira restricta strain DSM 18626]
ATGGCCACTGCCAAGCCAGCAAAAAAACCCGCAGGAGCGAAGGAGTTCACCTTTCTCTGGGAGGGCAAGAACAAGGACGG
CAAGGATGTCAGGGGCGAACTGCGGGCCACCAGCGAGGCAGTCGCGCAGGCAACCCTCCGCCGGCAGGGCGTGCGCATCA
CCAAGATCAGCAAGCAGCGTTTCAAGACCGGAAAGAAGATCACCGACAAGGACATCACGCTGTTCACGCGCCAGCTGGCG
ACGATGATGAAAGCCGGCGTGCCGCTGCTGCAGGCCTTCGACATCGTCGGCCGCGGCCACTCGAACCCCTCGGTCGGCAA
GCTGCTGCTCGACATCAAGGCCGACGTCGAGACCGGCTCGTCGCTATCGCAGGCCTTCCGCAAGTATCCGCTCCACTTCG
ATGCCCTCTTCTGCAACCTCGTCGCCGCCGGCGAACAGGCCGGCATCCTGGAAGCGCTGCTCGACCGCCTGGCGACCTAC
AAGGAAAAAATGCAGGCGCTGAAGGCCAAGATCAAGTCGGCACTGTTCTACCCGATGGCGATCCTGATCATGGCCTTCGT
CATCACCGCGGTGATCATGATCTTCGTGATCCCGGCGTTCAAGGAAGTCTTCAAGAGCTTCGGCGCCGACCTGCCGGCGC
CGACGGTGATGGTGATCGCCATGTCCGACTATTTCGTCGAGTATTGGTGGGCGATCTTCGGCTCGATCGGCGGCGGCATT
TATGCCTTTATCCAGGCCTACAAGCGCTCCGAGGCGATGCAGATCGCCTTCGACCGCTACGCGCTGCGCATCCCGGTCTT
TGGCGACGTCATCCGCAAATCCACGGTCGCCCGGTGGACGCGGACGCTGGCGACGATGTTCGCCGCCGGCGTGCCGCTGG
TCGAGTCGCTCGACTCGGTCGGCGGCGCCTCCGGCAACTACGTCTACAAGGTCGCCACCCGCCAGATCCAGAGCGAAGTC
AGCACCGGCACCAACCTGACGACGGCGATGCAGAACAGCAAGCTGTTCGACAACATGGTGATCCAGATGGTCGCCATCGG
CGAAGAGTCCGGCGCCCTCGACTCGATGCTGGAAAAGGTCGCCGGCTTCCTCGAGGCCGAGGTCGACGATGCGGTCGAGG
CGCTCTCCAGCCTGATGGAACCGATGATCATGGCCGTCCTCGGCGTCCTCATCGGCGGCCTCGTGATCGCCATGTACCTG
CCGATCTTCAAGCTGGGTTCCGTCGTCGGATGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(75-198)

Type II secretion system (T2SS), protein F (T2SSF)

(279-401)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

55.138

97.317

0.537
  pilC Acinetobacter baumannii D1279779

54.25

97.561

0.529
  pilC Legionella pneumophila strain ERS1305867

53.266

97.073

0.517
  pilC Acinetobacter baylyi ADP1

52.141

96.829

0.505
  pilG Neisseria meningitidis 44/76-A

51.889

96.829

0.502
  pilG Neisseria gonorrhoeae MS11

51.889

96.829

0.502
  pilC Vibrio cholerae strain A1552

40.201

97.073

0.39
  pilC Vibrio campbellii strain DS40M4

39.356

98.537

0.388