Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   IWH25_RS07280 Genome accession   NZ_CP064781
Coordinates   1475697..1477412 (-) Length   571 a.a.
NCBI ID   WP_203388657.1    Uniprot ID   A0A974Y572
Organism   Azospira restricta strain DSM 18626     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1470697..1482412
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IWH25_RS07255 (IWH25_07250) aspS 1470758..1472572 (-) 1815 WP_203388653.1 aspartate--tRNA ligase -
  IWH25_RS07260 (IWH25_07255) - 1472599..1473213 (-) 615 WP_203389179.1 DUF502 domain-containing protein -
  IWH25_RS07265 (IWH25_07260) - 1473213..1473479 (-) 267 WP_203388654.1 FmdB family zinc ribbon protein -
  IWH25_RS07270 (IWH25_07265) pilD 1473593..1474444 (-) 852 WP_203388655.1 prepilin peptidase Machinery gene
  IWH25_RS07275 (IWH25_07270) pilC 1474441..1475673 (-) 1233 WP_203388656.1 type II secretion system F family protein Machinery gene
  IWH25_RS07280 (IWH25_07275) pilB 1475697..1477412 (-) 1716 WP_203388657.1 type IV-A pilus assembly ATPase PilB Machinery gene
  IWH25_RS07285 (IWH25_07280) - 1477562..1478401 (-) 840 WP_203388658.1 cytochrome C assembly family protein -
  IWH25_RS07290 (IWH25_07285) ffh 1478432..1479793 (+) 1362 WP_203388659.1 signal recognition particle protein -
  IWH25_RS07295 (IWH25_07290) - 1479805..1480410 (-) 606 WP_203388660.1 lytic transglycosylase domain-containing protein -
  IWH25_RS07300 (IWH25_07295) - 1480401..1482116 (-) 1716 WP_203388661.1 proline--tRNA ligase -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 61751.96 Da        Isoelectric Point: 5.2097

>NTDB_id=504702 IWH25_RS07280 WP_203388657.1 1475697..1477412(-) (pilB) [Azospira restricta strain DSM 18626]
MAANPQQAPLSGLARALVQAGRLKETEAESLLAQAAANGSSLIEQIANAKKMSAQEVATFAAETFGYPLFDLAAFDDAHI
PANAVDRKLISSHRVIPLHKRGNRLTIATADPTNLRALDEIRFQTSMIVDPVVVEEPKLLPLVKKLSESAEETLKSIASE
DINLEFTDEEAQAATEEAASLEVDDAPVVKFIQKMLLDAINDGASDIHFEPYEKFYRIRFRVDGILREIATPPLVIKDKI
ASRIKVISRLNIAEKRVPQDGRMKLVLSKNRAIDFRVSTLPTLHGEKIVLRILDPTSATLGIDALGYDPDQKEALLDAIN
RPYGMVLVTGPTGSGKTVSLYTCLNILNQPGINISTAEDPAEIPLPGINQVNVDDKAGLTFAAALKSFLRQDPDIIMVGE
IRDLETAEIAVKAAQTGHMVLSTLHTNDAPQTLTRLMNMGVPLFNIASSVLLITAQRLARRLCTCKVPHEAPTEALVNAG
FTEADLDGSWTLYGPGSCDRCKGSGYKGRVGIYQVMPVTEEIQRIILAGGTALDIAAQAAREGVKDLRRSGLIKAMQGVT
SLEEVLSTTNA

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=504702 IWH25_RS07280 WP_203388657.1 1475697..1477412(-) (pilB) [Azospira restricta strain DSM 18626]
ATGGCAGCCAACCCGCAACAAGCGCCACTCAGCGGCCTCGCCCGGGCCCTCGTGCAAGCCGGCCGCCTCAAGGAAACCGA
GGCGGAAAGCCTGCTTGCGCAGGCGGCCGCCAATGGCAGCTCTTTGATCGAACAGATCGCCAACGCAAAGAAGATGTCGG
CGCAGGAAGTTGCCACTTTTGCCGCAGAAACCTTCGGCTACCCGCTTTTCGACCTGGCCGCCTTCGACGACGCGCACATT
CCGGCAAACGCTGTCGACCGCAAGCTGATCAGCTCGCACCGGGTCATCCCGCTGCACAAACGCGGCAACCGGCTGACCAT
CGCGACCGCCGACCCGACCAATCTGCGGGCGCTCGACGAAATCCGTTTCCAGACCAGCATGATCGTCGACCCGGTGGTGG
TCGAGGAGCCCAAGCTGCTGCCGCTGGTCAAGAAGCTCTCGGAATCCGCGGAAGAAACGCTGAAGAGCATCGCCAGCGAG
GATATCAACCTCGAGTTCACCGACGAGGAAGCGCAGGCAGCGACGGAGGAGGCGGCCTCGCTCGAAGTGGACGACGCGCC
GGTCGTCAAGTTCATCCAGAAGATGCTGCTCGACGCGATCAACGACGGCGCCTCGGACATCCACTTCGAGCCTTACGAGA
AGTTCTACCGCATCCGCTTCCGCGTCGACGGCATCTTGCGCGAAATCGCCACGCCGCCGCTGGTGATCAAGGACAAGATC
GCCTCGCGGATCAAGGTCATCTCGCGCCTCAACATCGCCGAGAAACGGGTCCCGCAGGACGGCCGAATGAAGCTGGTGCT
GTCCAAGAACCGCGCGATCGACTTCCGGGTCAGCACGCTGCCGACGCTGCACGGTGAAAAGATCGTGCTGCGCATTCTCG
ACCCGACCAGCGCCACGCTGGGCATCGACGCCCTCGGCTACGACCCGGACCAGAAGGAAGCCCTGCTCGACGCGATCAAC
CGCCCCTACGGCATGGTGCTGGTTACCGGGCCGACCGGCTCCGGCAAGACGGTATCGCTCTACACCTGCCTGAACATCCT
CAACCAGCCCGGGATCAACATCTCGACGGCGGAGGACCCGGCGGAAATTCCGCTGCCGGGCATCAACCAGGTCAACGTCG
ACGACAAGGCCGGACTGACTTTCGCCGCGGCGCTGAAGTCCTTCCTGCGCCAGGATCCGGACATCATCATGGTCGGCGAA
ATCCGCGACCTGGAGACCGCCGAGATTGCGGTCAAGGCCGCGCAGACCGGCCACATGGTGCTGTCGACGCTGCACACCAA
CGATGCGCCGCAAACGCTGACCCGCCTGATGAACATGGGCGTGCCGCTGTTCAACATCGCCTCCAGCGTGCTGCTGATCA
CCGCCCAGCGACTGGCGCGGCGGCTATGCACCTGCAAGGTTCCGCACGAAGCACCGACCGAAGCGCTGGTCAACGCCGGT
TTCACCGAGGCCGACCTCGACGGCTCGTGGACGCTGTACGGGCCGGGCAGTTGCGATCGCTGCAAGGGCTCCGGCTACAA
GGGGCGGGTCGGCATCTATCAGGTGATGCCGGTCACCGAGGAAATCCAGCGCATCATCCTCGCCGGCGGAACCGCGCTCG
ACATCGCCGCCCAGGCCGCCCGCGAGGGGGTCAAGGACCTGCGCCGCTCGGGCCTGATCAAGGCGATGCAGGGCGTCACT
TCGCTGGAAGAAGTGCTGAGCACCACCAACGCATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(51-151)

Type II/IV secretion system protein (T2SSE)

(194-461)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.42

100

0.555
  pilB Acinetobacter baylyi ADP1

54.625

100

0.548
  pilB Legionella pneumophila strain ERS1305867

53.415

100

0.534
  pilF Neisseria gonorrhoeae MS11

52.734

99.299

0.524
  pilB Vibrio cholerae strain A1552

49.027

98.949

0.485
  pilB Vibrio parahaemolyticus RIMD 2210633

47.695

98.774

0.471
  pilB Vibrio campbellii strain DS40M4

47.434

98.949

0.469
  pilF Thermus thermophilus HB27

41.217

100

0.415
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.394

98.249

0.387