Detailed information NTG0504700

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: IWH25_RS07270
• Genome accession: NZ_CP064781
• Coordinates: 1473593..1474444 (-)
• Length: 283 a.a.
• NCBI ID: WP_203388655.1
• Uniprot ID: A0A974SRE9
• Organism: Azospira restricta strain DSM 18626
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1468593..1479444

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
IWH25_RS07235 (IWH25_07230)	-	1468882..1469397 (+)	516	WP_203388649.1	DoxX family protein	-
IWH25_RS07240 (IWH25_07235)	nudB	1469471..1469914 (-)	444	WP_203388650.1	dihydroneopterin triphosphate diphosphatase	-
IWH25_RS07245 (IWH25_07240)	-	1469911..1470366 (-)	456	WP_203388651.1	barstar family protein	-
IWH25_RS07250 (IWH25_07245)	-	1470377..1470754 (-)	378	WP_203388652.1	ribonuclease domain-containing protein	-
IWH25_RS07255 (IWH25_07250)	aspS	1470758..1472572 (-)	1815	WP_203388653.1	aspartate--tRNA ligase	-
IWH25_RS07260 (IWH25_07255)	-	1472599..1473213 (-)	615	WP_203389179.1	DUF502 domain-containing protein	-
IWH25_RS07265 (IWH25_07260)	-	1473213..1473479 (-)	267	WP_203388654.1	FmdB family zinc ribbon protein	-
IWH25_RS07270 (IWH25_07265)	pilD	1473593..1474444 (-)	852	WP_203388655.1	prepilin peptidase	Machinery gene
IWH25_RS07275 (IWH25_07270)	pilC	1474441..1475673 (-)	1233	WP_203388656.1	type II secretion system F family protein	Machinery gene
IWH25_RS07280 (IWH25_07275)	pilB	1475697..1477412 (-)	1716	WP_203388657.1	type IV-A pilus assembly ATPase PilB	Machinery gene
IWH25_RS07285 (IWH25_07280)	-	1477562..1478401 (-)	840	WP_203388658.1	cytochrome C assembly family protein	-

Sequence

Protein

• Length: 283 a.a.
• Molecular weight: 30131.63 Da
• Isoelectric Point: 7.6424

>NTDB_id=504700 IWH25_RS07270 WP_203388655.1 1473593..1474444(-) (pilD) [Azospira restricta strain DSM 18626]
MIALFADPVVFTTAAGLLGLIVGSFLNVVIHRLPLMMERDWQCQCAELRGEAAPPAEPLSLSQPRSRCPHCGHPISALEN
IPLLSWLLLRGRCSACGAGIALRYPLVETASGLLAAFAAWHFGFGWAAAGAIVFGWAMIALTGIDFDTQLLPDSITLPLL
WLGLAFNLFGTYTSLASAVIGAMAGYLSLWSVYWGFKLATGKEGMGYGDFKLLAAIGAWLGWQALPLTILFSSLVGAVVG
IVLIVAARHGRNVPIPFGPYLAAAGLLALFWGRPLTQSYLGLF

Nucleotide

• Length: 852 bp

>NTDB_id=504700 IWH25_RS07270 WP_203388655.1 1473593..1474444(-) (pilD) [Azospira restricta strain DSM 18626]
ATGATCGCGCTGTTCGCCGACCCCGTCGTCTTCACCACGGCGGCGGGGCTGCTCGGCCTGATCGTCGGCAGCTTCCTCAA
CGTCGTCATCCATCGCCTGCCGCTGATGATGGAGCGGGACTGGCAGTGCCAGTGCGCGGAGCTGCGCGGGGAGGCGGCGC
CGCCGGCGGAGCCGCTCTCGCTGTCGCAGCCGCGCTCGCGCTGCCCGCATTGCGGCCATCCGATCTCGGCACTGGAGAAC
ATTCCGCTGCTCAGCTGGCTGCTCCTGCGCGGCAGGTGCTCGGCCTGCGGCGCCGGCATTGCGCTCCGCTACCCGCTGGT
CGAGACGGCCAGCGGCCTGCTGGCGGCATTCGCCGCCTGGCATTTCGGCTTCGGCTGGGCGGCCGCGGGTGCCATCGTCT
TCGGCTGGGCGATGATCGCGCTGACCGGCATCGACTTCGACACGCAGCTGCTGCCCGACAGCATCACGCTGCCGCTGCTC
TGGCTGGGCCTCGCCTTCAATCTGTTCGGCACCTACACCTCGCTCGCCTCGGCGGTGATCGGCGCGATGGCCGGCTACCT
GTCGCTGTGGTCGGTCTACTGGGGGTTCAAGCTGGCGACCGGCAAGGAGGGCATGGGCTACGGCGACTTCAAGCTGCTCG
CGGCGATCGGCGCCTGGCTCGGCTGGCAGGCGCTGCCGCTGACCATCCTGTTCTCGTCGCTGGTCGGCGCCGTCGTCGGC
ATCGTGCTGATCGTCGCCGCCCGCCACGGGCGCAACGTTCCGATCCCGTTCGGCCCCTACCTGGCGGCAGCCGGGCTGCT
GGCGCTGTTCTGGGGCCGCCCGCTGACGCAATCCTATCTCGGGCTCTTTTGA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (133-241)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (17-122)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	57.455	97.173	0.558
pilD	Vibrio campbellii strain DS40M4	53.986	97.527	0.527
pilD	Acinetobacter nosocomialis M2	51.481	95.406	0.491
pilD	Acinetobacter baumannii D1279779	50.741	95.406	0.484
pilD	Neisseria gonorrhoeae MS11	51.779	89.399	0.463