Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   IUJ52_RS10730 Genome accession   NZ_CP064379
Coordinates   2279919..2281607 (-) Length   562 a.a.
NCBI ID   WP_004729083.1    Uniprot ID   -
Organism   Vibrio furnissii strain PartQ-Vfurnissii-RM8376     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2274919..2286607
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IUJ52_RS10705 (IUJ52_10700) yacG 2276054..2276251 (-) 198 WP_004729092.1 DNA gyrase inhibitor YacG -
  IUJ52_RS10710 (IUJ52_10705) zapD 2276309..2277049 (-) 741 WP_004729090.1 cell division protein ZapD -
  IUJ52_RS10715 (IUJ52_10710) coaE 2277082..2277690 (-) 609 WP_004729089.1 dephospho-CoA kinase -
  IUJ52_RS10720 (IUJ52_10715) pilD 2277693..2278562 (-) 870 WP_004729087.1 A24 family peptidase Machinery gene
  IUJ52_RS10725 (IUJ52_10720) pilC 2278614..2279840 (-) 1227 WP_004729085.1 type II secretion system F family protein Machinery gene
  IUJ52_RS10730 (IUJ52_10725) pilB 2279919..2281607 (-) 1689 WP_004729083.1 type IV-A pilus assembly ATPase PilB Machinery gene
  IUJ52_RS10735 (IUJ52_10730) - 2281613..2282062 (-) 450 WP_004729081.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  IUJ52_RS10740 (IUJ52_10735) nadC 2282309..2283196 (-) 888 WP_004729079.1 carboxylating nicotinate-nucleotide diphosphorylase -
  IUJ52_RS10745 (IUJ52_10740) ampD 2283345..2283899 (+) 555 WP_004729077.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  IUJ52_RS10750 (IUJ52_10745) fldB 2283935..2284456 (-) 522 WP_004729075.1 flavodoxin FldB -
  IUJ52_RS10755 (IUJ52_10750) xerD 2284652..2285506 (+) 855 WP_231623439.1 site-specific tyrosine recombinase XerD -
  IUJ52_RS10760 (IUJ52_10755) dsbC 2285523..2286287 (+) 765 WP_004729072.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -

Sequence

Protein


Download         Length: 562 a.a.        Molecular weight: 62058.19 Da        Isoelectric Point: 6.1994

>NTDB_id=501454 IUJ52_RS10730 WP_004729083.1 2279919..2281607(-) (pilB) [Vibrio furnissii strain PartQ-Vfurnissii-RM8376]
MLTNLIAILRQAKLISPTQEQAVIDHIHASGATVPEALIDLAIFHHHDLTEQLSQIFGLPETDLTRFDYAPLCQQLGLRE
LITRYQALPLHSNGQILTLAVADPTHLQVEDEFRFATGLQVELVLADHKALHAAIRRLYGRSLATPGTQGKEISQDELAD
LVQISDDEKTTIEDLSQDDSPVSRFINQILLDAVRKGASDIHFEPYESHYRIRLRCDGILVETQQPASHLSRRLAARIKI
LSKLDIAERRLPQDGRIKLRLDADTAIDMRVSTLPTLWGEKIVLRLLDSSAANLNIDKLGYNEQQKQLYLAALTKPQGMI
LMTGPTGSGKTVSLYTGLRILNTEQVNISTAEDPVEINLPGINQVQVQPKIGFGFAQALRSFLRQDPDVVMVGEIRDLET
AEIAIKAAQTGHLVLSTLHTNSAAETVIRLANMGIEPFNLASSLSLIIAQRLARRLCPHCKVAITPPAALQQQFGLLAED
AVYEAHPSGCTECTNGYSGRVGIYEVMAFHPALADAVMQRASIHQIETIAQQHGMQPLLQSGIEKLRAGITSFSELQRVL
YF

Nucleotide


Download         Length: 1689 bp        

>NTDB_id=501454 IUJ52_RS10730 WP_004729083.1 2279919..2281607(-) (pilB) [Vibrio furnissii strain PartQ-Vfurnissii-RM8376]
ATGCTGACCAACCTTATCGCCATTCTGCGTCAGGCTAAGTTGATCAGCCCAACGCAGGAACAGGCCGTGATCGATCATAT
TCACGCTTCCGGTGCCACGGTGCCGGAGGCGTTGATCGATTTGGCGATATTTCATCATCACGATTTAACCGAGCAACTGA
GTCAGATCTTTGGCCTACCCGAAACCGATCTCACTCGGTTTGATTACGCGCCATTGTGCCAGCAGTTGGGACTTCGAGAA
CTCATCACCCGCTACCAAGCCCTGCCGCTGCACAGCAATGGCCAAATCCTGACGTTGGCGGTTGCTGACCCAACTCACCT
GCAAGTCGAAGACGAGTTTCGCTTTGCCACCGGATTACAGGTGGAACTGGTGCTCGCCGATCACAAGGCGCTGCATGCCG
CCATTCGCCGCTTGTATGGGCGCAGCCTTGCGACGCCGGGGACGCAAGGCAAAGAGATCAGTCAGGATGAATTGGCGGAT
CTGGTACAGATCTCTGACGACGAAAAAACCACCATCGAAGATCTCAGCCAAGATGATTCACCGGTCAGTCGTTTTATCAA
CCAGATCCTACTCGATGCCGTGCGCAAAGGTGCGTCCGACATTCACTTTGAGCCGTATGAGTCCCACTACCGTATTCGTC
TACGCTGTGACGGCATTCTGGTCGAAACCCAACAACCAGCCAGTCACCTCAGCCGACGCTTGGCAGCCCGAATCAAAATT
TTGTCGAAATTAGATATTGCCGAGCGCCGCCTCCCGCAAGATGGACGCATCAAACTGCGCTTGGATGCCGACACCGCGAT
TGATATGCGTGTATCGACCTTGCCAACATTGTGGGGTGAAAAGATTGTTCTGCGCCTACTCGACAGCAGTGCGGCCAACC
TCAACATCGACAAACTCGGCTACAACGAGCAGCAAAAGCAGCTCTACCTCGCGGCCCTCACCAAACCGCAAGGCATGATC
TTAATGACCGGCCCAACCGGCAGCGGCAAAACTGTGTCGCTCTACACCGGATTACGCATTCTCAATACCGAACAAGTGAA
CATCTCCACCGCCGAAGATCCGGTGGAAATCAACCTGCCCGGCATCAATCAGGTGCAAGTTCAACCCAAAATCGGCTTTG
GCTTTGCACAAGCGCTGCGCTCGTTTCTGCGTCAAGATCCCGATGTGGTGATGGTGGGCGAAATCCGCGATCTAGAAACG
GCAGAAATCGCCATTAAGGCCGCGCAAACCGGGCACTTGGTGCTCTCAACCTTGCACACCAACTCGGCCGCGGAAACCGT
GATTCGTTTAGCCAACATGGGCATTGAACCCTTTAACCTTGCCTCATCATTGAGCCTGATCATTGCGCAGCGGCTGGCCC
GCCGTTTGTGCCCGCATTGCAAGGTCGCCATCACGCCCCCAGCTGCGCTGCAGCAGCAATTTGGCTTGCTCGCCGAAGAT
GCCGTGTACGAAGCACATCCATCGGGTTGCACGGAATGCACCAATGGGTATAGCGGCCGCGTGGGCATTTATGAAGTGAT
GGCGTTTCATCCCGCCTTGGCGGACGCGGTGATGCAGCGCGCCAGCATTCATCAAATCGAAACCATTGCCCAACAGCACG
GCATGCAACCCCTGCTGCAATCGGGGATTGAAAAACTGCGCGCGGGCATCACCAGTTTTAGTGAATTACAGCGAGTACTC
TACTTTTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(39-142)

Type II/IV secretion system protein (T2SSE)

(188-455)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

82.384

100

0.824
  pilB Vibrio campbellii strain DS40M4

71.886

100

0.719
  pilB Vibrio parahaemolyticus RIMD 2210633

71.886

100

0.719
  pilB Legionella pneumophila strain ERS1305867

52.519

91.815

0.482
  pilB Acinetobacter baumannii D1279779

51.06

92.349

0.472
  pilB Acinetobacter baylyi ADP1

50.097

91.993

0.461
  pilF Neisseria gonorrhoeae MS11

44.858

100

0.45
  pilB Haemophilus influenzae 86-028NP

39.658

93.772

0.372
  pilB/pilB1 Synechocystis sp. PCC 6803

37.636

97.865

0.368
  pilF Thermus thermophilus HB27

36.655

100

0.367