Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   H9J98_RS03155 Genome accession   NZ_CP064041
Coordinates   652119..652571 (-) Length   150 a.a.
NCBI ID   WP_024702454.1    Uniprot ID   A0A9Q3UHP8
Organism   Vibrio parahaemolyticus strain XMO116     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 647119..657571
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H9J98_RS03135 (H9J98_03130) coaE 647635..648249 (-) 615 WP_025631392.1 dephospho-CoA kinase -
  H9J98_RS03140 (H9J98_03135) pilD 648250..649119 (-) 870 WP_176296457.1 A24 family peptidase Machinery gene
  H9J98_RS03145 (H9J98_03140) pilC 649184..650407 (-) 1224 WP_005479682.1 type II secretion system F family protein Machinery gene
  H9J98_RS03150 (H9J98_03145) pilB 650433..652118 (-) 1686 WP_176296458.1 type IV-A pilus assembly ATPase PilB Machinery gene
  H9J98_RS03155 (H9J98_03150) pilA 652119..652571 (-) 453 WP_024702454.1 pilin Machinery gene
  H9J98_RS03160 (H9J98_03155) nadC 652834..653721 (-) 888 WP_228085838.1 carboxylating nicotinate-nucleotide diphosphorylase -
  H9J98_RS03165 (H9J98_03160) ampD 653814..654365 (+) 552 WP_005479703.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  H9J98_RS03170 (H9J98_03165) pdhR 654771..655538 (+) 768 WP_005462576.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 150 a.a.        Molecular weight: 15716.95 Da        Isoelectric Point: 9.0482

>NTDB_id=498647 H9J98_RS03155 WP_024702454.1 652119..652571(-) (pilA) [Vibrio parahaemolyticus strain XMO116]
MKHSKQKKQQGFTLIELMIVVAVIGVLASIAIPQYQKYVAKSEVASALATMTGVKTNVEAYAVENGEFPDTGKENDLGVP
SSIPSGNIAFAKTASAATGTITFTFKTSGVSNLITGKKFALARDGNGTWTCDGTASNAVTDDLLPKNCRP

Nucleotide


Download         Length: 453 bp        

>NTDB_id=498647 H9J98_RS03155 WP_024702454.1 652119..652571(-) (pilA) [Vibrio parahaemolyticus strain XMO116]
ATGAAACACAGTAAACAGAAAAAACAACAAGGTTTTACCTTGATTGAATTGATGATTGTCGTGGCGGTAATTGGCGTACT
AGCATCGATTGCGATTCCCCAATACCAAAAGTATGTGGCAAAGTCAGAAGTAGCATCTGCTCTTGCAACAATGACTGGCG
TAAAAACGAACGTAGAAGCATATGCAGTTGAAAATGGCGAATTCCCGGATACAGGTAAAGAGAATGATCTGGGTGTTCCG
AGTTCCATTCCCTCAGGAAATATAGCGTTTGCTAAAACGGCATCTGCAGCAACTGGTACCATTACTTTCACATTTAAAAC
TAGTGGTGTAAGTAACCTAATTACAGGTAAAAAGTTCGCTTTAGCAAGAGATGGTAACGGTACTTGGACTTGTGACGGCA
CTGCAAGTAATGCAGTTACAGATGATCTTCTGCCGAAAAACTGCCGTCCATAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(40-149)

Prokaryotic N-terminal methylation motif (N_methyl)

(7-31)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio cholerae strain A1552

49.351

100

0.507
  pilA Vibrio cholerae C6706

49.351

100

0.507
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

49.351

100

0.507
  pilA Vibrio parahaemolyticus RIMD 2210633

44.966

99.333

0.447
  pilA Pseudomonas aeruginosa PAK

43.709

100

0.44
  pilA Acinetobacter baumannii strain A118

43.357

95.333

0.413
  pilA Acinetobacter nosocomialis M2

40.426

94

0.38
  pilE Neisseria gonorrhoeae MS11

35.669

100

0.373