Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   IMX18_RS25935 Genome accession   NZ_CP063456
Coordinates   5617070..5618338 (+) Length   422 a.a.
NCBI ID   WP_217189988.1    Uniprot ID   -
Organism   Pseudomonas shirazica strain A28     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5612070..5623338
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IMX18_RS25920 pilM 5614719..5615600 (+) 882 WP_054572203.1 type IV pilus biogenesis protein PilM -
  IMX18_RS25925 - 5615597..5616133 (+) 537 WP_139657891.1 PilN domain-containing protein -
  IMX18_RS25930 - 5616145..5617146 (+) 1002 WP_043936291.1 pilus assembly protein PilP -
  IMX18_RS25935 pilQ 5617070..5618338 (+) 1269 WP_217189988.1 type IV pilus secretin PilQ Machinery gene
  IMX18_RS25940 aroK 5618343..5618861 (+) 519 WP_015272136.1 shikimate kinase AroK -
  IMX18_RS25945 aroB 5618958..5620055 (+) 1098 WP_015272135.1 3-dehydroquinate synthase -
  IMX18_RS25950 - 5620071..5621705 (+) 1635 WP_100412792.1 SPOR domain-containing protein -

Sequence

Protein


Download         Length: 422 a.a.        Molecular weight: 46120.97 Da        Isoelectric Point: 5.0056

>NTDB_id=495750 IMX18_RS25935 WP_217189988.1 5617070..5618338(+) (pilQ) [Pseudomonas shirazica strain A28]
MKRLMKWMLAALLPFPLASAMPYQGEPLSLNFQDVEVRSVLQVLADYAGVNLVASDAVQGSVTLRLEDVPWDQALDLVLR
SKGLARRQEGNVLLVAPAAEFAAQSVDAHVGQALDAQLQPLRRELLPIHHAKAAELAELLLASLADDGILNGRGSLSVDE
RTNTLVVYQPADRLAELRQLVAQLDVPVRQVAIEARIVEANVDYEKSLGVRWGGPLYVENPRPGKDLFVDLGVERVGGSI
GLGLLRGDVLLDLELSAMEKSGNGEIISQPKVVTADKETARILKGTEVPYQETSKSGATSVAFREASLSLEVTPQITPDN
KVIMTVRVTKDEPDYVNALNNVPPIRKNEVNAKVRVTDRETIVIGGVYSTSQNNVVDKVPFFGDLPYVGRLFRRDALQEK
KSELLVFLTPRIMSDQAIAVSR

Nucleotide


Download         Length: 1269 bp        

>NTDB_id=495750 IMX18_RS25935 WP_217189988.1 5617070..5618338(+) (pilQ) [Pseudomonas shirazica strain A28]
ATGAAAAGATTGATGAAATGGATGCTGGCAGCCCTGCTGCCGTTCCCGCTGGCGTCGGCAATGCCTTATCAGGGTGAACC
ACTGTCGCTGAACTTCCAGGATGTGGAAGTGCGCTCGGTGCTGCAGGTGCTGGCTGACTATGCCGGTGTCAACCTGGTTG
CCAGCGATGCCGTGCAGGGTAGCGTCACCTTGCGCCTTGAGGATGTTCCATGGGACCAGGCGCTGGATCTGGTGCTGCGC
AGCAAGGGCTTGGCCCGGCGACAGGAGGGCAATGTGCTGTTGGTGGCGCCCGCGGCGGAGTTTGCCGCGCAGTCTGTCGA
TGCCCATGTGGGCCAGGCGCTGGATGCACAGTTGCAGCCGTTGCGTCGGGAGCTGTTGCCCATTCATCACGCCAAGGCCG
CCGAGCTGGCAGAGCTGCTGCTGGCGAGCCTGGCGGATGACGGCATCCTCAACGGTCGGGGCAGCCTGAGTGTCGATGAG
CGCACCAATACGCTGGTGGTGTACCAGCCGGCAGACCGCCTTGCCGAACTGAGGCAGCTGGTGGCGCAGCTGGATGTGCC
GGTGCGTCAGGTGGCGATCGAGGCACGTATCGTCGAGGCCAACGTCGATTACGAGAAAAGCCTGGGGGTGCGCTGGGGTG
GGCCGCTGTATGTCGAGAACCCGCGCCCGGGCAAGGATCTGTTCGTCGATCTCGGGGTGGAGCGGGTGGGCGGTAGCATC
GGTCTGGGCTTGTTGCGTGGCGATGTGCTGCTCGACCTGGAACTCAGTGCCATGGAAAAGAGCGGCAACGGCGAAATCAT
CTCGCAGCCCAAGGTGGTCACGGCCGACAAGGAAACGGCCAGGATCCTCAAAGGCACCGAGGTGCCGTACCAGGAAACCA
GCAAGAGCGGCGCCACCTCGGTAGCGTTCCGCGAAGCCTCGTTGTCGCTGGAAGTGACCCCGCAGATCACTCCGGACAAC
AAGGTGATCATGACCGTCAGGGTGACCAAGGACGAGCCGGATTACGTCAATGCCTTGAACAACGTACCGCCGATCCGCAA
GAACGAGGTCAATGCGAAGGTTCGCGTGACGGACCGAGAAACGATCGTGATCGGTGGCGTGTACTCGACTTCGCAAAACA
ATGTGGTCGACAAGGTGCCATTTTTCGGCGATCTGCCGTATGTTGGGCGGCTGTTTCGACGCGATGCATTACAAGAGAAA
AAATCCGAGCTGCTGGTCTTCCTGACTCCGCGTATCATGAGTGACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(51-97)

Bacterial type II/III secretion system short domain (Secretin_N)

(125-190)

Bacterial type II and III secretion system protein (Secretin)

(257-413)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

59.174

100

0.611
  pilQ Acinetobacter baumannii D1279779

41.703

100

0.453
  pilQ Acinetobacter baumannii strain A118

41.703

100

0.453
  comQ Acinetobacter baylyi ADP1

42.035

100

0.45
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

40.783

100

0.419
  pilQ Vibrio cholerae strain A1552

40.783

100

0.419
  pilQ Legionella pneumophila strain ERS1305867

42.105

99.052

0.417
  pilQ Legionella pneumophila strain Lp02

42.105

99.052

0.417
  pilQ Vibrio campbellii strain DS40M4

40.509

100

0.415
  pilQ Neisseria gonorrhoeae strain FA1090

36.603

99.052

0.363