Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   INP90_RS00260 Genome accession   NZ_CP063124
Coordinates   52928..54307 (+) Length   459 a.a.
NCBI ID   WP_197559687.1    Uniprot ID   -
Organism   Haemophilus parainfluenzae strain M1C113_1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 47928..59307
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  INP90_RS00230 (INP90_00230) - 48221..49072 (-) 852 WP_197559683.1 thymidylate synthase -
  INP90_RS00235 (INP90_00235) lgt 49083..49892 (-) 810 WP_197559684.1 prolipoprotein diacylglyceryl transferase -
  INP90_RS00240 (INP90_00240) - 49901..50695 (-) 795 WP_197559685.1 sulfite exporter TauE/SafE family protein -
  INP90_RS00245 (INP90_00245) rppH 50695..51288 (-) 594 WP_049379955.1 RNA pyrophosphohydrolase -
  INP90_RS00250 (INP90_00250) ampD 51772..52335 (-) 564 WP_197559686.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  INP90_RS00255 (INP90_00255) pilA 52478..52921 (+) 444 WP_005697917.1 prepilin peptidase-dependent pilin Machinery gene
  INP90_RS00260 (INP90_00260) pilB 52928..54307 (+) 1380 WP_197559687.1 GspE/PulE family protein Machinery gene
  INP90_RS00265 (INP90_00265) pilC 54316..55539 (+) 1224 WP_197559688.1 type II secretion system F family protein Machinery gene
  INP90_RS00270 (INP90_00270) pilD 55536..56213 (+) 678 WP_197559689.1 prepilin peptidase Machinery gene
  INP90_RS00275 (INP90_00275) coaE 56299..56919 (+) 621 WP_197559690.1 dephospho-CoA kinase -
  INP90_RS00280 (INP90_00280) yacG 56912..57118 (+) 207 WP_005695505.1 DNA gyrase inhibitor YacG -
  INP90_RS00285 (INP90_00285) - 57115..57396 (+) 282 WP_197559691.1 GNAT family N-acetyltransferase -
  INP90_RS00290 (INP90_00290) rhlB 57487..58746 (+) 1260 WP_197559692.1 ATP-dependent RNA helicase RhlB -
  INP90_RS00295 (INP90_00295) mreD 58787..59275 (-) 489 WP_197559693.1 rod shape-determining protein MreD -

Sequence

Protein


Download         Length: 459 a.a.        Molecular weight: 52010.28 Da        Isoelectric Point: 6.2291

>NTDB_id=493188 INP90_RS00260 WP_197559687.1 52928..54307(+) (pilB) [Haemophilus parainfluenzae strain M1C113_1]
MAYQAIAKNGEIYHISPQYWQQNQQQQALLLRYFALPLKEDGHHLWLAVDSLNNLAACETFAFLSGKLVEPILFETTQLK
QLLQSLAPKANQIEEQTTFYHHSEDESTANLSNEDEPVIQLLNSIFETALQKNASDIHLETQSNGLLIRLRIDGVLQPQP
VISKSLANRVISRLKLLAKLDISETRLPQDGRFQFKTTFSDILDFRLSTLPTHWGEKAVLRLQQNKPVQLSFAELGMTQN
QQIQFQHALSQPQGLILVTGPTGSGKSISLYTALQWLNTPDKHIMTAEDPVEIELEGIIQTQVNPQIGLDFSRLLRTFLR
QDPDIIMLGEIRDEESALMALRAAQTGHLVLSTLHTNDAISAISRLQQLGIQSHEIENSLLLVIAQRLVRKRCLKCGQHF
SDSCDCHQGYQGRIGVYQFLQCENNRYQTDFESLYQSALEKVKDHVTDLAEIQRVLGKK

Nucleotide


Download         Length: 1380 bp        

>NTDB_id=493188 INP90_RS00260 WP_197559687.1 52928..54307(+) (pilB) [Haemophilus parainfluenzae strain M1C113_1]
ATGGCCTATCAAGCAATCGCAAAAAATGGCGAGATTTATCATATCTCGCCACAATATTGGCAACAAAACCAACAGCAACA
AGCTTTGCTGCTGCGTTACTTTGCGCTCCCTCTTAAAGAAGACGGACACCATTTATGGCTTGCGGTGGATTCGCTGAATA
ATCTTGCGGCTTGTGAAACCTTTGCTTTTTTAAGTGGAAAATTGGTTGAACCGATTTTATTTGAAACGACTCAACTCAAG
CAACTTTTACAATCCCTTGCCCCTAAAGCCAATCAAATAGAAGAACAAACGACGTTTTATCATCATTCAGAAGACGAAAG
CACGGCTAATTTATCTAATGAAGATGAACCCGTTATTCAATTATTAAACAGTATTTTTGAAACAGCCCTGCAAAAAAATG
CCTCCGATATTCATTTAGAAACTCAGTCAAATGGGCTCTTAATTCGTTTACGTATTGATGGTGTACTCCAACCTCAGCCG
GTAATCAGTAAATCGCTCGCCAATCGTGTAATTTCTCGTTTAAAACTCTTAGCCAAACTGGATATCAGTGAAACTCGGCT
ACCACAAGATGGCCGCTTTCAATTCAAAACGACATTCTCAGATATTTTAGATTTCCGCCTTTCTACCTTGCCCACGCATT
GGGGAGAAAAAGCGGTATTACGATTACAACAAAATAAACCTGTTCAGTTAAGTTTTGCTGAACTTGGCATGACACAAAAC
CAACAAATCCAATTTCAACATGCACTCAGCCAACCACAAGGTTTAATTCTTGTGACAGGACCAACGGGAAGCGGAAAAAG
TATCTCGCTTTATACCGCACTTCAATGGCTCAATACGCCAGATAAACATATTATGACGGCAGAAGATCCCGTTGAAATTG
AATTAGAAGGTATTATTCAAACTCAAGTGAATCCGCAAATCGGTTTAGATTTTAGCCGATTACTTCGTACATTTTTACGA
CAAGATCCCGACATTATTATGCTTGGTGAAATTCGAGACGAAGAAAGTGCATTGATGGCATTAAGAGCGGCTCAAACAGG
TCATCTAGTACTTTCTACCTTACATACCAACGATGCGATCTCAGCCATTTCACGCTTACAACAATTAGGCATTCAATCTC
ACGAAATTGAAAATAGTCTCTTATTGGTTATCGCACAGCGATTGGTACGTAAACGTTGCTTAAAGTGCGGTCAACATTTT
AGTGATTCTTGCGATTGCCATCAGGGGTATCAAGGGCGTATTGGGGTATATCAATTTTTACAATGCGAAAATAACCGTTA
CCAAACTGATTTTGAGTCACTTTATCAAAGCGCTTTAGAAAAAGTAAAAGATCACGTCACCGATCTCGCCGAAATTCAAC
GCGTATTAGGTAAAAAATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(124-391)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(26-88)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae 86-028NP

78.319

98.475

0.771
  pilB Haemophilus influenzae Rd KW20

77.434

98.475

0.763
  pilB Glaesserella parasuis strain SC1401

59.292

98.475

0.584
  pilB Vibrio cholerae strain A1552

41.667

100

0.436
  pilB Vibrio campbellii strain DS40M4

40.833

100

0.427
  pilB Vibrio parahaemolyticus RIMD 2210633

40.625

100

0.425
  pilB Acinetobacter baylyi ADP1

38.632

100

0.418
  pilB Acinetobacter baumannii D1279779

39.425

100

0.418
  pilB Legionella pneumophila strain ERS1305867

38.65

100

0.412
  pilF Neisseria gonorrhoeae MS11

39.394

93.464

0.368
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

35.518

100

0.366
  pilF Thermus thermophilus HB27

38.695

93.464

0.362