Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   INP90_RS00255 Genome accession   NZ_CP063124
Coordinates   52478..52921 (+) Length   147 a.a.
NCBI ID   WP_005697917.1    Uniprot ID   A0A369YN52
Organism   Haemophilus parainfluenzae strain M1C113_1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 47478..57921
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  INP90_RS00225 (INP90_00225) tadA 47693..48211 (-) 519 WP_197559682.1 tRNA adenosine(34) deaminase TadA -
  INP90_RS00230 (INP90_00230) - 48221..49072 (-) 852 WP_197559683.1 thymidylate synthase -
  INP90_RS00235 (INP90_00235) lgt 49083..49892 (-) 810 WP_197559684.1 prolipoprotein diacylglyceryl transferase -
  INP90_RS00240 (INP90_00240) - 49901..50695 (-) 795 WP_197559685.1 sulfite exporter TauE/SafE family protein -
  INP90_RS00245 (INP90_00245) rppH 50695..51288 (-) 594 WP_049379955.1 RNA pyrophosphohydrolase -
  INP90_RS00250 (INP90_00250) ampD 51772..52335 (-) 564 WP_197559686.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  INP90_RS00255 (INP90_00255) pilA 52478..52921 (+) 444 WP_005697917.1 prepilin peptidase-dependent pilin Machinery gene
  INP90_RS00260 (INP90_00260) pilB 52928..54307 (+) 1380 WP_197559687.1 GspE/PulE family protein Machinery gene
  INP90_RS00265 (INP90_00265) pilC 54316..55539 (+) 1224 WP_197559688.1 type II secretion system F family protein Machinery gene
  INP90_RS00270 (INP90_00270) pilD 55536..56213 (+) 678 WP_197559689.1 prepilin peptidase Machinery gene
  INP90_RS00275 (INP90_00275) coaE 56299..56919 (+) 621 WP_197559690.1 dephospho-CoA kinase -
  INP90_RS00280 (INP90_00280) yacG 56912..57118 (+) 207 WP_005695505.1 DNA gyrase inhibitor YacG -
  INP90_RS00285 (INP90_00285) - 57115..57396 (+) 282 WP_197559691.1 GNAT family N-acetyltransferase -

Sequence

Protein


Download         Length: 147 a.a.        Molecular weight: 15151.42 Da        Isoelectric Point: 8.4674

>NTDB_id=493187 INP90_RS00255 WP_005697917.1 52478..52921(+) (pilA) [Haemophilus parainfluenzae strain M1C113_1]
MKLTFSKPLHKGFTLIELMIVIAIIAILATIAIPSYQNYTKKAAISELLQASAPYKSDVELCVYSTNAPTNCSGGSNGIA
ADITTAKGYVKSITTKSGVITVTGNGALDGISYSLTATGSTSSGVTWTTACPSNADLFPAGFCSPTK

Nucleotide


Download         Length: 444 bp        

>NTDB_id=493187 INP90_RS00255 WP_005697917.1 52478..52921(+) (pilA) [Haemophilus parainfluenzae strain M1C113_1]
ATGAAACTCACTTTTTCTAAACCTTTACATAAAGGTTTTACGTTAATTGAATTGATGATCGTGATTGCGATTATTGCTAT
TCTCGCGACGATCGCCATTCCGTCTTATCAAAATTATACCAAAAAAGCAGCTATTTCCGAATTGTTACAAGCTTCTGCAC
CTTATAAATCTGATGTGGAGTTATGTGTCTATAGTACCAATGCCCCGACAAACTGTTCAGGTGGTTCAAATGGCATAGCA
GCAGACATCACTACTGCAAAAGGCTATGTTAAATCCATTACCACGAAATCGGGTGTGATTACGGTAACAGGAAATGGCGC
ATTGGATGGGATCAGTTATTCTTTAACGGCGACAGGCTCAACCTCATCAGGTGTGACTTGGACAACTGCTTGCCCAAGCA
ATGCGGATTTATTCCCTGCGGGTTTCTGCTCTCCTACCAAATAG

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(10-32)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A369YN52

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

75.694

97.959

0.741
  pilA Haemophilus influenzae Rd KW20

73.611

97.959

0.721
  pilA Glaesserella parasuis strain SC1401

56.081

100

0.565
  pilA Vibrio campbellii strain DS40M4

41.096

99.32

0.408
  pilE Neisseria gonorrhoeae MS11

41.985

89.116

0.374
  pilA2 Legionella pneumophila str. Paris

42.188

87.075

0.367