Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   INP91_RS08720 Genome accession   NZ_CP063123
Coordinates   1808641..1810020 (+) Length   459 a.a.
NCBI ID   WP_197545828.1    Uniprot ID   -
Organism   Haemophilus parainfluenzae strain M1C120_2     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1803641..1815020
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  INP91_RS08690 (INP91_08685) - 1803934..1804785 (-) 852 WP_070582536.1 thymidylate synthase -
  INP91_RS08695 (INP91_08690) lgt 1804798..1805604 (-) 807 WP_070582537.1 prolipoprotein diacylglyceryl transferase -
  INP91_RS08700 (INP91_08695) - 1805613..1806407 (-) 795 WP_197545827.1 sulfite exporter TauE/SafE family protein -
  INP91_RS08705 (INP91_08700) rppH 1806407..1807000 (-) 594 WP_070582539.1 RNA pyrophosphohydrolase -
  INP91_RS08710 (INP91_08705) ampD 1807485..1808048 (-) 564 WP_070582540.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  INP91_RS08715 (INP91_08710) pilA 1808191..1808634 (+) 444 WP_049379953.1 prepilin peptidase-dependent pilin Machinery gene
  INP91_RS08720 (INP91_08715) pilB 1808641..1810020 (+) 1380 WP_197545828.1 GspE/PulE family protein Machinery gene
  INP91_RS08725 (INP91_08720) pilC 1810029..1811252 (+) 1224 WP_197545829.1 type II secretion system F family protein Machinery gene
  INP91_RS08730 (INP91_08725) pilD 1811249..1811926 (+) 678 WP_197545830.1 prepilin peptidase Machinery gene
  INP91_RS08735 (INP91_08730) coaE 1812015..1812635 (+) 621 WP_197545831.1 dephospho-CoA kinase -
  INP91_RS08740 (INP91_08735) yacG 1812628..1812834 (+) 207 WP_005695505.1 DNA gyrase inhibitor YacG -
  INP91_RS08745 (INP91_08740) - 1812831..1813112 (+) 282 WP_014064065.1 GNAT family N-acetyltransferase -
  INP91_RS08750 (INP91_08745) rhlB 1813203..1814462 (+) 1260 WP_197545832.1 ATP-dependent RNA helicase RhlB -
  INP91_RS08755 (INP91_08750) mreD 1814501..1814989 (-) 489 WP_197545833.1 rod shape-determining protein MreD -

Sequence

Protein


Download         Length: 459 a.a.        Molecular weight: 52092.40 Da        Isoelectric Point: 6.1486

>NTDB_id=493181 INP91_RS08720 WP_197545828.1 1808641..1810020(+) (pilB) [Haemophilus parainfluenzae strain M1C120_2]
MAYQAIAKNGEIYHISPQYWQQNQQQQALLLRYFALPLKEDEHHLWLAVDSLNNLAACETFAFLSGKLVEPILFETAELK
QLLQSLAPKANQIEEQTTFYHHSEDESTANLSNDDEPVIQLLNGIFETALQKNASDIHLETQPNGLLIRLRIDGVLQPQP
VISKSLANRVISRLKLLAKLDISETRLPQDGRFQFKTTFSDILDFRLSTLPTYWGEKAVLRLQRNKPVQLSFAELGMTQN
QQSQFQHALSQPQGLILVTGPTGSGKSISLYTALQWLNTPDKHIMTAEDPVEIELEGIIQTQVNPQIGLDFSRLLRTFLR
QDPDIIMLGEIRDEESALMALRAAQTGHLVLSTLHTNDTISAISRLQQLGIQSHEIENSLLLVIAQRLVRKRCLKCGQHF
CNSCDCHQGYQGRIGVYQFLQCENNRYQTDFDSLYQSALEKVKDHITDLAEIQRVLGKK

Nucleotide


Download         Length: 1380 bp        

>NTDB_id=493181 INP91_RS08720 WP_197545828.1 1808641..1810020(+) (pilB) [Haemophilus parainfluenzae strain M1C120_2]
ATGGCTTATCAAGCAATCGCAAAAAATGGCGAGATTTATCATATCTCGCCACAATATTGGCAACAAAACCAACAGCAACA
AGCTTTGCTGTTGCGTTACTTTGCGCTCCCCCTTAAAGAAGATGAACACCATTTATGGCTTGCGGTGGACTCACTCAATA
ATCTTGCAGCTTGTGAAACCTTTGCTTTTTTAAGTGGAAAATTAGTCGAACCGATTTTATTTGAAACGGCCGAACTCAAA
CAACTTTTACAATCCCTTGCGCCTAAAGCCAATCAAATCGAAGAGCAAACGACGTTTTATCATCATTCAGAAGACGAAAG
CACTGCCAATTTATCTAATGATGATGAGCCCGTTATTCAATTATTAAATGGTATTTTTGAAACAGCCCTGCAAAAAAATG
CCTCCGATATTCATTTAGAAACCCAGCCAAATGGGCTCTTAATTCGTTTACGTATTGATGGTGTACTCCAACCACAACCG
GTAATCAGTAAATCGCTCGCCAATCGTGTAATTTCTCGTTTAAAACTCTTAGCCAAACTGGATATCAGTGAAACTCGGCT
ACCACAAGATGGCCGCTTTCAATTCAAAACGACATTCTCCGATATTTTAGATTTCCGCCTTTCTACCTTACCTACCTATT
GGGGAGAAAAAGCGGTATTACGATTACAACGAAATAAACCTGTTCAGTTAAGTTTTGCTGAGCTTGGCATGACACAAAAC
CAACAAAGTCAATTTCAGCATGCACTCAGCCAACCTCAAGGTTTAATCCTGGTGACGGGACCAACGGGAAGCGGAAAAAG
TATCTCGCTTTATACCGCACTTCAATGGCTCAATACGCCAGATAAACATATTATGACGGCAGAAGATCCCGTTGAAATTG
AATTAGAAGGCATTATTCAAACTCAAGTGAATCCGCAAATCGGTTTAGATTTTAGCCGATTACTTCGTACATTTTTACGA
CAAGATCCCGACATTATTATGCTTGGTGAAATTCGAGATGAAGAAAGTGCATTGATGGCATTAAGAGCGGCTCAAACCGG
CCATTTAGTGCTTTCTACCTTGCATACCAATGATACCATCTCAGCCATTTCACGCTTGCAACAATTAGGCATTCAATCTC
ACGAAATTGAAAACAGTCTCTTATTAGTTATCGCACAACGATTGGTACGCAAACGTTGCTTAAAGTGCGGTCAACATTTT
TGTAATTCTTGTGATTGCCATCAGGGGTATCAAGGCCGTATTGGGGTATATCAATTTTTACAATGCGAAAACAACCGTTA
CCAAACTGATTTCGATTCACTTTATCAAAGCGCGTTAGAAAAAGTGAAAGATCACATCACTGATCTCGCCGAAATTCAAC
GCGTGTTAGGTAAAAAATAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(26-88)

Type II/IV secretion system protein (T2SSE)

(125-391)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae 86-028NP

77.876

98.475

0.767
  pilB Haemophilus influenzae Rd KW20

77.212

98.475

0.76
  pilB Glaesserella parasuis strain SC1401

59.424

98.257

0.584
  pilB Vibrio cholerae strain A1552

41.701

100

0.438
  pilB Vibrio campbellii strain DS40M4

41.079

100

0.431
  pilB Vibrio parahaemolyticus RIMD 2210633

40.871

100

0.429
  pilB Legionella pneumophila strain ERS1305867

39.059

100

0.416
  pilB Acinetobacter baylyi ADP1

37.626

100

0.407
  pilB Acinetobacter baumannii D1279779

38.398

100

0.407
  pilF Neisseria gonorrhoeae MS11

35.699

100

0.373