Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   INP96_RS00265 Genome accession   NZ_CP063116
Coordinates   52180..53559 (+) Length   459 a.a.
NCBI ID   WP_111327324.1    Uniprot ID   -
Organism   Haemophilus parainfluenzae strain M1C146_1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 47180..58559
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  INP96_RS00235 (INP96_00235) - 47472..48323 (-) 852 WP_197555886.1 thymidylate synthase -
  INP96_RS00240 (INP96_00240) lgt 48334..49143 (-) 810 WP_197555887.1 prolipoprotein diacylglyceryl transferase -
  INP96_RS00245 (INP96_00245) - 49152..49946 (-) 795 WP_197555888.1 sulfite exporter TauE/SafE family protein -
  INP96_RS00250 (INP96_00250) rppH 49946..50539 (-) 594 WP_197555889.1 RNA pyrophosphohydrolase -
  INP96_RS00255 (INP96_00255) ampD 51024..51587 (-) 564 WP_197555890.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  INP96_RS00260 (INP96_00260) pilA 51730..52173 (+) 444 WP_049369499.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  INP96_RS00265 (INP96_00265) pilB 52180..53559 (+) 1380 WP_111327324.1 GspE/PulE family protein Machinery gene
  INP96_RS00270 (INP96_00270) pilC 53568..54791 (+) 1224 WP_197555891.1 type II secretion system F family protein Machinery gene
  INP96_RS00275 (INP96_00275) pilD 54788..55465 (+) 678 WP_197555892.1 prepilin peptidase Machinery gene
  INP96_RS00280 (INP96_00280) coaE 55552..56172 (+) 621 WP_197555893.1 dephospho-CoA kinase -
  INP96_RS00285 (INP96_00285) yacG 56165..56371 (+) 207 WP_111327320.1 DNA gyrase inhibitor YacG -
  INP96_RS00290 (INP96_00290) - 56368..56649 (+) 282 WP_111327319.1 GNAT family N-acetyltransferase -
  INP96_RS00295 (INP96_00295) rhlB 56740..57999 (+) 1260 WP_197555894.1 ATP-dependent RNA helicase RhlB -
  INP96_RS00300 (INP96_00300) mreD 58041..58529 (-) 489 WP_111327318.1 rod shape-determining protein MreD -

Sequence

Protein


Download         Length: 459 a.a.        Molecular weight: 52006.26 Da        Isoelectric Point: 5.7472

>NTDB_id=492999 INP96_RS00265 WP_111327324.1 52180..53559(+) (pilB) [Haemophilus parainfluenzae strain M1C146_1]
MAYQAIAKNGEIYQISPQYWQQNQQQQALLLRYFALPIKEDEHHLWLAVDSLNNLAACETFAFLSGKLVEPILFETAQLK
QLLQSLAPKANQIEEQTTFYHHSEDESTANLSNEDEPVIQLLNSVFETALQKNASDIHLEIQPNGLLTRLRIDGVLQPQP
IISKSLANRVISRLKLLAKLDISETRLPQDGRFQFKTTFSDILDFRLSTLPTHWGEKAVLRLQQNKPVQLSFAELGMTKN
QQNQFQHALGQPQGLILVTGPTGSGKSISLYTALQWLNTPDKHIMTAEDPIEIELEGIIQTQVNPQIGLDFSRLLRTFLR
QDPDIIMLGEIRDEESALMALRAAQTGHLVLSTLHTNDALSAISRLQQLGIQSHEIENSLLLVIAQRLVRKRCLKCGQHF
SDSCDCHQGYQGRIGVYQFLQCENNCYQTDFDSLYQSALEKVKDNITDLDEIQRVLGKK

Nucleotide


Download         Length: 1380 bp        

>NTDB_id=492999 INP96_RS00265 WP_111327324.1 52180..53559(+) (pilB) [Haemophilus parainfluenzae strain M1C146_1]
ATGGCTTATCAAGCAATCGCAAAAAATGGCGAGATTTACCAAATCTCGCCACAATATTGGCAACAAAACCAACAGCAACA
AGCATTGCTGTTACGTTACTTTGCACTCCCTATTAAAGAAGACGAACACCATTTATGGCTTGCAGTAGATTCGCTGAATA
ATCTTGCAGCTTGTGAAACCTTTGCTTTTTTAAGTGGAAAATTAGTTGAACCGATTTTATTTGAAACGGCCCAACTTAAA
CAACTTTTACAATCCCTTGCACCGAAAGCCAATCAAATAGAAGAGCAAACGACGTTTTATCATCATTCAGAAGATGAAAG
CACTGCCAATTTATCTAATGAAGATGAACCTGTTATTCAATTATTAAACAGTGTTTTTGAAACTGCCCTACAAAAAAATG
CCTCCGATATTCATTTAGAAATTCAGCCAAATGGGCTCTTAACTCGTTTACGTATTGATGGCGTACTCCAACCACAGCCG
ATTATCAGTAAATCACTCGCCAATCGTGTAATTTCTCGTTTAAAACTCTTAGCCAAACTGGATATCAGTGAAACTCGGCT
ACCACAAGATGGCCGCTTTCAATTCAAAACGACATTCTCCGATATTTTAGATTTCCGCCTTTCTACCTTGCCCACGCATT
GGGGAGAAAAAGCGGTATTACGGTTACAACAAAATAAACCTGTTCAGTTAAGTTTTGCTGAACTTGGCATGACAAAAAAC
CAACAAAATCAATTTCAACATGCACTCGGTCAACCTCAGGGTTTAATCCTCGTGACTGGGCCAACGGGAAGCGGAAAAAG
TATTTCGCTTTATACTGCACTTCAATGGCTCAATACGCCAGATAAACATATCATGACGGCAGAAGATCCCATTGAAATTG
AATTGGAAGGTATTATTCAAACTCAAGTAAATCCACAAATCGGTTTAGATTTTAGCCGATTACTTCGTACATTTTTACGG
CAAGACCCCGACATTATTATGCTTGGTGAAATTCGAGATGAAGAAAGTGCATTAATGGCATTAAGAGCAGCTCAAACCGG
CCATTTAGTGCTTTCGACCTTACATACCAACGATGCCCTCTCAGCAATTTCACGCTTGCAACAACTAGGCATTCAATCTC
ATGAAATTGAAAATAGTCTCTTATTAGTTATCGCACAGAGATTGGTGCGTAAACGTTGTTTAAAGTGCGGTCAACATTTT
AGTGATTCTTGCGATTGCCATCAGGGGTATCAAGGGCGTATTGGGGTGTATCAATTTTTACAATGTGAAAATAACTGTTA
CCAAACTGATTTCGATTCACTTTATCAAAGTGCATTAGAAAAAGTAAAAGATAACATCACCGATCTTGATGAAATTCAAC
GTGTGTTAGGTAAAAAATAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(25-88)

Type II/IV secretion system protein (T2SSE)

(125-391)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae 86-028NP

77.434

98.475

0.763
  pilB Haemophilus influenzae Rd KW20

76.264

99.129

0.756
  pilB Glaesserella parasuis strain SC1401

59.292

98.475

0.584
  pilB Vibrio cholerae strain A1552

41.458

100

0.434
  pilB Vibrio campbellii strain DS40M4

40.417

100

0.423
  pilB Vibrio parahaemolyticus RIMD 2210633

40.208

100

0.42
  pilB Acinetobacter baumannii D1279779

38.604

100

0.41
  pilB Acinetobacter baylyi ADP1

37.827

100

0.41
  pilB Legionella pneumophila strain ERS1305867

38.037

100

0.405
  pilF Neisseria gonorrhoeae MS11

36.82

100

0.383
  pilF Thermus thermophilus HB27

36.383

100

0.373
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

35.095

100

0.362