Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   INP99_RS00260 Genome accession   NZ_CP063111
Coordinates   51998..53377 (+) Length   459 a.a.
NCBI ID   WP_197542327.1    Uniprot ID   -
Organism   Haemophilus parainfluenzae strain M1C152_1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 46998..58377
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  INP99_RS00230 (INP99_00230) - 47288..48139 (-) 852 WP_049364941.1 thymidylate synthase -
  INP99_RS00235 (INP99_00235) lgt 48150..48959 (-) 810 WP_197542326.1 prolipoprotein diacylglyceryl transferase -
  INP99_RS00240 (INP99_00240) - 48968..49762 (-) 795 WP_177989464.1 sulfite exporter TauE/SafE family protein -
  INP99_RS00245 (INP99_00245) rppH 49762..50355 (-) 594 WP_178160903.1 RNA pyrophosphohydrolase -
  INP99_RS00250 (INP99_00250) ampD 50840..51403 (-) 564 WP_054419755.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  INP99_RS00255 (INP99_00255) pilA 51548..51991 (+) 444 WP_005697917.1 prepilin peptidase-dependent pilin Machinery gene
  INP99_RS00260 (INP99_00260) pilB 51998..53377 (+) 1380 WP_197542327.1 GspE/PulE family protein Machinery gene
  INP99_RS00265 (INP99_00265) pilC 53386..54609 (+) 1224 WP_197542328.1 type II secretion system F family protein Machinery gene
  INP99_RS00270 (INP99_00270) pilD 54606..55283 (+) 678 WP_197542329.1 prepilin peptidase Machinery gene
  INP99_RS00275 (INP99_00275) coaE 55371..55991 (+) 621 WP_197542330.1 dephospho-CoA kinase -
  INP99_RS00280 (INP99_00280) yacG 55984..56190 (+) 207 WP_005695505.1 DNA gyrase inhibitor YacG -
  INP99_RS00285 (INP99_00285) - 56187..56468 (+) 282 WP_178160891.1 GNAT family N-acetyltransferase -
  INP99_RS00290 (INP99_00290) rhlB 56559..57818 (+) 1260 WP_197542331.1 ATP-dependent RNA helicase RhlB -
  INP99_RS00295 (INP99_00295) mreD 57860..58348 (-) 489 WP_111327318.1 rod shape-determining protein MreD -

Sequence

Protein


Download         Length: 459 a.a.        Molecular weight: 52059.32 Da        Isoelectric Point: 5.8776

>NTDB_id=492905 INP99_RS00260 WP_197542327.1 51998..53377(+) (pilB) [Haemophilus parainfluenzae strain M1C152_1]
MAYQAIAKNGEIYQISPQYWQQNQQQQALLLRYFALPLKEDEHHLWLAVDSLNNLAACETFAFLSGKLVEPILFETHQLK
QLLQSLAPKANQIEEQATFYHHSEDESTANLSNEDEPVIQLLNGIFETALQKNASDIHLETQPNGLLVRLRIDGVLQPQP
IISKSLANRVISRLKLLAKLDISETRLPQDGRFQFKTTFSDILDFRLSTLPTHWGEKAVLRLQQNKPVQLSFAELGMTQK
QQSQFQHALSQPQGLILVTGPTGSGKSISLYTALQWLNTPDKHIMTAEDPVEIELEGIIQTQVNPQIGLDFSRLLRTFLR
QDPDIIMLGEIRDEESALMALRAAQTGHLVLSTLHTNDALSAISRLQQLGIQSHEIENSLLLVIAQRLVRKRCLKCSQHF
SESCDCHQGYQGRIGVYQFLQCENNCYQTDFDSLYQSALEKVKDNITDLDEIQRVLGKK

Nucleotide


Download         Length: 1380 bp        

>NTDB_id=492905 INP99_RS00260 WP_197542327.1 51998..53377(+) (pilB) [Haemophilus parainfluenzae strain M1C152_1]
ATGGCCTATCAAGCAATCGCAAAAAATGGCGAGATTTATCAGATCTCGCCACAATATTGGCAACAAAACCAACAGCAACA
AGCTTTGCTGTTGCGTTACTTTGCGCTCCCTCTTAAAGAAGACGAACACCATTTATGGCTTGCAGTAGATTCGCTGAATA
ATCTTGCAGCTTGTGAAACCTTTGCCTTTTTAAGTGGAAAATTAGTTGAACCAATTTTATTTGAAACACATCAACTCAAA
CAACTTTTACAATCCCTTGCACCGAAAGCCAATCAAATAGAAGAACAAGCGACGTTTTATCATCATTCAGAAGACGAAAG
CACTGCCAATTTATCTAATGAAGATGAACCCGTTATTCAATTATTAAATGGTATTTTTGAAACCGCCCTGCAAAAAAATG
CCTCCGATATTCATTTAGAAACTCAGCCAAATGGGCTTTTAGTTCGTTTGCGTATTGATGGCGTACTACAACCACAGCCG
ATTATCAGTAAATCACTCGCCAATCGTGTGATTTCTCGTTTAAAACTCTTAGCCAAACTGGATATCAGTGAAACTCGGCT
ACCACAAGATGGCCGTTTTCAATTCAAAACGACATTCTCCGATATTTTAGATTTCCGCCTTTCTACCTTGCCCACGCATT
GGGGAGAAAAAGCGGTATTACGGTTACAACAAAATAAACCTGTTCAGTTAAGTTTTGCTGAACTTGGCATGACACAAAAA
CAACAAAGTCAATTTCAACATGCACTCAGCCAACCGCAAGGTTTAATTCTTGTGACTGGACCAACGGGAAGTGGAAAAAG
TATCTCACTTTATACCGCACTTCAATGGCTCAATACGCCAGATAAACATATTATGACGGCAGAGGATCCCGTTGAAATTG
AATTAGAAGGCATTATTCAAACTCAAGTGAATCCGCAAATTGGTTTAGATTTTAGCCGATTACTTCGTACATTTTTACGA
CAAGACCCCGACATTATTATGCTTGGTGAAATTCGAGATGAAGAAAGTGCATTAATGGCATTAAGAGCAGCTCAAACCGG
CCATTTAGTGCTTTCGACCTTACATACCAACGATGCCCTCTCAGCAATTTCACGCTTGCAACAACTAGGCATTCAATCTC
ATGAAATTGAAAATAGTCTCTTATTAGTTATCGCACAGCGATTGGTGCGTAAACGTTGCTTAAAGTGCAGTCAACATTTT
AGTGAGTCTTGCGATTGCCATCAGGGGTATCAAGGGCGTATTGGGGTGTATCAATTTTTACAATGTGAAAATAACTGTTA
CCAAACTGATTTCGATTCACTTTATCAAAGTGCATTAGAAAAAGTAAAAGATAACATCACCGATCTTGATGAAATTCAAC
GTGTGTTAGGTAAAAAATAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(25-88)

Type II/IV secretion system protein (T2SSE)

(125-391)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae 86-028NP

77.363

99.129

0.767
  pilB Haemophilus influenzae Rd KW20

76.703

99.129

0.76
  pilB Glaesserella parasuis strain SC1401

59.513

98.475

0.586
  pilB Vibrio campbellii strain DS40M4

41.322

100

0.436
  pilB Vibrio cholerae strain A1552

41.458

100

0.434
  pilB Vibrio parahaemolyticus RIMD 2210633

41.25

100

0.431
  pilB Acinetobacter baumannii D1279779

39.63

100

0.42
  pilB Legionella pneumophila strain ERS1305867

38.855

100

0.414
  pilB Acinetobacter baylyi ADP1

37.827

100

0.41
  pilF Thermus thermophilus HB27

38.681

99.129

0.383
  pilF Neisseria gonorrhoeae MS11

36.611

100

0.381
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

35.307

100

0.364