Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   INP99_RS00255 Genome accession   NZ_CP063111
Coordinates   51548..51991 (+) Length   147 a.a.
NCBI ID   WP_005697917.1    Uniprot ID   A0A369YN52
Organism   Haemophilus parainfluenzae strain M1C152_1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 46548..56991
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  INP99_RS00225 (INP99_00225) tadA 46760..47278 (-) 519 WP_197542325.1 tRNA adenosine(34) deaminase TadA -
  INP99_RS00230 (INP99_00230) - 47288..48139 (-) 852 WP_049364941.1 thymidylate synthase -
  INP99_RS00235 (INP99_00235) lgt 48150..48959 (-) 810 WP_197542326.1 prolipoprotein diacylglyceryl transferase -
  INP99_RS00240 (INP99_00240) - 48968..49762 (-) 795 WP_177989464.1 sulfite exporter TauE/SafE family protein -
  INP99_RS00245 (INP99_00245) rppH 49762..50355 (-) 594 WP_178160903.1 RNA pyrophosphohydrolase -
  INP99_RS00250 (INP99_00250) ampD 50840..51403 (-) 564 WP_054419755.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  INP99_RS00255 (INP99_00255) pilA 51548..51991 (+) 444 WP_005697917.1 prepilin peptidase-dependent pilin Machinery gene
  INP99_RS00260 (INP99_00260) pilB 51998..53377 (+) 1380 WP_197542327.1 GspE/PulE family protein Machinery gene
  INP99_RS00265 (INP99_00265) pilC 53386..54609 (+) 1224 WP_197542328.1 type II secretion system F family protein Machinery gene
  INP99_RS00270 (INP99_00270) pilD 54606..55283 (+) 678 WP_197542329.1 prepilin peptidase Machinery gene
  INP99_RS00275 (INP99_00275) coaE 55371..55991 (+) 621 WP_197542330.1 dephospho-CoA kinase -
  INP99_RS00280 (INP99_00280) yacG 55984..56190 (+) 207 WP_005695505.1 DNA gyrase inhibitor YacG -
  INP99_RS00285 (INP99_00285) - 56187..56468 (+) 282 WP_178160891.1 GNAT family N-acetyltransferase -

Sequence

Protein


Download         Length: 147 a.a.        Molecular weight: 15151.42 Da        Isoelectric Point: 8.4674

>NTDB_id=492904 INP99_RS00255 WP_005697917.1 51548..51991(+) (pilA) [Haemophilus parainfluenzae strain M1C152_1]
MKLTFSKPLHKGFTLIELMIVIAIIAILATIAIPSYQNYTKKAAISELLQASAPYKSDVELCVYSTNAPTNCSGGSNGIA
ADITTAKGYVKSITTKSGVITVTGNGALDGISYSLTATGSTSSGVTWTTACPSNADLFPAGFCSPTK

Nucleotide


Download         Length: 444 bp        

>NTDB_id=492904 INP99_RS00255 WP_005697917.1 51548..51991(+) (pilA) [Haemophilus parainfluenzae strain M1C152_1]
ATGAAACTGACTTTTTCTAAACCCTTACATAAAGGTTTTACGTTAATTGAATTGATGATCGTGATTGCGATTATTGCTAT
TCTCGCGACGATCGCCATTCCGTCTTATCAAAATTATACCAAAAAAGCAGCCATCTCCGAATTGTTGCAAGCTTCTGCAC
CTTATAAATCTGATGTGGAATTATGTGTCTATAGCACCAATGCCCCAACAAACTGTTCTGGTGGTTCAAATGGCATTGCA
GCAGACATCACTACTGCAAAAGGCTATGTTAAATCCATTACCACGAAATCGGGTGTGATTACGGTAACAGGAAATGGCGC
ATTGGATGGGATCAGTTATTCTTTAACGGCGACAGGCTCAACCTCATCAGGTGTCACCTGGACAACTGCTTGCCCAAGCA
ATGCGGATTTATTCCCAGCTGGTTTCTGCTCTCCTACCAAATAG

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(10-32)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A369YN52

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

75.694

97.959

0.741
  pilA Haemophilus influenzae Rd KW20

73.611

97.959

0.721
  pilA Glaesserella parasuis strain SC1401

56.081

100

0.565
  pilA Vibrio campbellii strain DS40M4

41.096

99.32

0.408
  pilE Neisseria gonorrhoeae MS11

41.985

89.116

0.374
  pilA2 Legionella pneumophila str. Paris

42.188

87.075

0.367