Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   INQ00_RS00255 Genome accession   NZ_CP063110
Coordinates   51064..52443 (+) Length   459 a.a.
NCBI ID   WP_197546949.1    Uniprot ID   -
Organism   Haemophilus parainfluenzae strain M1C160_1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 46064..57443
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  INQ00_RS00225 (INQ00_00225) - 46356..47207 (-) 852 WP_049364941.1 thymidylate synthase -
  INQ00_RS00230 (INQ00_00230) lgt 47218..48027 (-) 810 WP_197546944.1 prolipoprotein diacylglyceryl transferase -
  INQ00_RS00235 (INQ00_00235) - 48036..48830 (-) 795 WP_197546945.1 sulfite exporter TauE/SafE family protein -
  INQ00_RS00240 (INQ00_00240) rppH 48961..49423 (-) 463 Protein_47 RNA pyrophosphohydrolase -
  INQ00_RS00245 (INQ00_00245) ampD 49906..50469 (-) 564 WP_197546947.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  INQ00_RS00250 (INQ00_00250) pilA 50614..51057 (+) 444 WP_197546948.1 prepilin peptidase-dependent pilin Machinery gene
  INQ00_RS00255 (INQ00_00255) pilB 51064..52443 (+) 1380 WP_197546949.1 GspE/PulE family protein Machinery gene
  INQ00_RS00260 (INQ00_00260) pilC 52452..53675 (+) 1224 WP_197546950.1 type II secretion system F family protein Machinery gene
  INQ00_RS00265 (INQ00_00265) pilD 53672..54349 (+) 678 WP_197546951.1 prepilin peptidase Machinery gene
  INQ00_RS00270 (INQ00_00270) coaE 54436..55056 (+) 621 WP_197546952.1 dephospho-CoA kinase -
  INQ00_RS00275 (INQ00_00275) yacG 55049..55255 (+) 207 WP_197546953.1 DNA gyrase inhibitor YacG -
  INQ00_RS00280 (INQ00_00280) - 55252..55533 (+) 282 WP_197546954.1 GNAT family N-acetyltransferase -
  INQ00_RS00285 (INQ00_00285) rhlB 55624..56883 (+) 1260 WP_197546955.1 ATP-dependent RNA helicase RhlB -
  INQ00_RS00290 (INQ00_00290) mreD 56925..57413 (-) 489 WP_111327318.1 rod shape-determining protein MreD -

Sequence

Protein


Download         Length: 459 a.a.        Molecular weight: 52123.36 Da        Isoelectric Point: 6.0196

>NTDB_id=492874 INQ00_RS00255 WP_197546949.1 51064..52443(+) (pilB) [Haemophilus parainfluenzae strain M1C160_1]
MAYQAIAKNGEIYQISPQYWQQNQQQQALLLRYFALPLKEDEHHLWLAVDSLNNLAACETFAFLSGKLVEPILFETHQLK
QLLQSLAPKANQIEEQATFYHHSEDESTANLSNEDEPVIQLLNGIFETALQKNASDIHLETQPNGLLVRLRIDGVLQPQP
IISKSLANRVISRLKLLAKLDISETRLPQDGRFQFKTTFSDILDFRLSTLPTHWGEKAVLRLQQNKPVQLSFAELGMTQN
QQNQFQHALGQPQGLILVTGPTGSGKSISLYTALQWLNTPDKHIMTAEDPVEIELEGIIQTQVNPQIGLDFSRLLRTFLR
QDPDIIMLGEIRDEESALMALRAAQTGHLVLSTLHTNDAISAISRLQQLGIQSHEIENSLLLVIAQRLVRKRCLKCSQHF
SESCDCHQGYRGRIGVYQFLQCENNRYQTDFDSLYQSALEKVKDQVTDLDEIQRVLGKK

Nucleotide


Download         Length: 1380 bp        

>NTDB_id=492874 INQ00_RS00255 WP_197546949.1 51064..52443(+) (pilB) [Haemophilus parainfluenzae strain M1C160_1]
ATGGCCTATCAAGCAATCGCAAAAAATGGCGAGATTTATCAGATCTCGCCACAATATTGGCAACAAAACCAACAGCAACA
AGCATTGCTGTTGCGTTACTTTGCGCTACCACTTAAAGAAGATGAACACCATTTATGGCTTGCGGTGGATTCGCTGAATA
ATCTTGCGGCTTGTGAAACCTTTGCTTTTTTAAGTGGAAAATTGGTTGAGCCAATTTTATTTGAAACACATCAACTCAAA
CAACTTTTACAATCCCTTGCACCGAAAGCCAATCAAATAGAAGAACAAGCGACGTTTTATCATCATTCAGAAGACGAAAG
CACTGCCAATTTATCTAATGAAGATGAACCCGTTATTCAATTATTAAATGGTATTTTTGAAACCGCCCTGCAAAAAAATG
CCTCCGATATTCATTTAGAAACTCAGCCAAATGGCCTTTTGGTTCGTTTGCGTATTGATGGCGTACTACAACCACAGCCG
ATTATCAGTAAATCACTCGCCAATCGTGTGATTTCTCGTTTAAAACTCTTAGCCAAACTGGATATCAGTGAAACTCGGCT
ACCACAAGATGGCCGTTTTCAATTCAAAACGACATTCTCCGATATTTTAGATTTCCGCCTTTCTACCTTGCCCACGCATT
GGGGAGAAAAAGCGGTATTACGATTGCAACAAAATAAACCTGTTCAGTTAAGTTTTGCTGAATTGGGTATGACACAAAAC
CAACAAAATCAATTTCAACATGCACTCGGTCAACCTCAGGGTTTAATCCTTGTAACGGGACCAACAGGAAGTGGAAAAAG
TATCTCACTTTATACCGCACTTCAATGGCTCAATACGCCAGATAAACATATTATGACGGCAGAGGATCCCGTTGAAATTG
AATTAGAAGGCATCATTCAAACTCAAGTAAATCCACAAATTGGTTTAGATTTTAGTCGATTACTTCGCACATTTTTACGA
CAAGACCCCGACATTATTATGCTTGGTGAAATTCGAGATGAAGAAAGTGCATTAATGGCATTAAGAGCAGCTCAAACTGG
CCATTTAGTGCTTTCTACCTTACATACCAACGATGCCATCTCAGCAATTTCACGCTTACAGCAATTAGGCATTCAATCTC
ACGAAATTGAAAATAGTCTCTTATTAGTTATCGCACAGCGATTGGTGCGTAAACGTTGCTTAAAGTGCAGTCAACATTTT
AGTGAGTCTTGCGATTGCCATCAGGGATATCGGGGACGTATTGGGGTGTATCAATTTTTACAATGTGAAAACAACCGTTA
CCAAACCGATTTTGACTCACTTTATCAAAGCGCATTAGAAAAAGTAAAAGATCAAGTCACCGATCTCGATGAAATTCAAC
GCGTGTTAGGCAAAAAATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(125-391)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(25-88)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae 86-028NP

78.097

98.475

0.769
  pilB Haemophilus influenzae Rd KW20

77.434

98.475

0.763
  pilB Glaesserella parasuis strain SC1401

59.735

98.475

0.588
  pilB Vibrio campbellii strain DS40M4

41.116

100

0.434
  pilB Vibrio cholerae strain A1552

41.25

100

0.431
  pilB Vibrio parahaemolyticus RIMD 2210633

40.833

100

0.427
  pilB Acinetobacter baumannii D1279779

39.836

100

0.423
  pilB Legionella pneumophila strain ERS1305867

38.855

100

0.414
  pilB Acinetobacter baylyi ADP1

38.028

100

0.412
  pilF Neisseria gonorrhoeae MS11

36.402

100

0.379
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

35.307

100

0.364