Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   INQ00_RS00250 Genome accession   NZ_CP063110
Coordinates   50614..51057 (+) Length   147 a.a.
NCBI ID   WP_197546948.1    Uniprot ID   -
Organism   Haemophilus parainfluenzae strain M1C160_1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 45614..56057
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  INQ00_RS00220 (INQ00_00220) tadA 45828..46346 (-) 519 WP_152682592.1 tRNA adenosine(34) deaminase TadA -
  INQ00_RS00225 (INQ00_00225) - 46356..47207 (-) 852 WP_049364941.1 thymidylate synthase -
  INQ00_RS00230 (INQ00_00230) lgt 47218..48027 (-) 810 WP_197546944.1 prolipoprotein diacylglyceryl transferase -
  INQ00_RS00235 (INQ00_00235) - 48036..48830 (-) 795 WP_197546945.1 sulfite exporter TauE/SafE family protein -
  INQ00_RS00240 (INQ00_00240) rppH 48961..49423 (-) 463 Protein_47 RNA pyrophosphohydrolase -
  INQ00_RS00245 (INQ00_00245) ampD 49906..50469 (-) 564 WP_197546947.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  INQ00_RS00250 (INQ00_00250) pilA 50614..51057 (+) 444 WP_197546948.1 prepilin peptidase-dependent pilin Machinery gene
  INQ00_RS00255 (INQ00_00255) pilB 51064..52443 (+) 1380 WP_197546949.1 GspE/PulE family protein Machinery gene
  INQ00_RS00260 (INQ00_00260) pilC 52452..53675 (+) 1224 WP_197546950.1 type II secretion system F family protein Machinery gene
  INQ00_RS00265 (INQ00_00265) pilD 53672..54349 (+) 678 WP_197546951.1 prepilin peptidase Machinery gene
  INQ00_RS00270 (INQ00_00270) coaE 54436..55056 (+) 621 WP_197546952.1 dephospho-CoA kinase -
  INQ00_RS00275 (INQ00_00275) yacG 55049..55255 (+) 207 WP_197546953.1 DNA gyrase inhibitor YacG -
  INQ00_RS00280 (INQ00_00280) - 55252..55533 (+) 282 WP_197546954.1 GNAT family N-acetyltransferase -

Sequence

Protein


Download         Length: 147 a.a.        Molecular weight: 15181.44 Da        Isoelectric Point: 8.4674

>NTDB_id=492873 INQ00_RS00250 WP_197546948.1 50614..51057(+) (pilA) [Haemophilus parainfluenzae strain M1C160_1]
MKLTFSKPLHKGFTLIELMIVIAIIAILATIAIPSYQNYTKKAAISELLQASAPYKSDVELCVYSTNAPTNCSGGSNGIT
ADITTAKGYVKSITTKSGVITVTGNGALDGISYSLTATGSTSSGVTWTTACPSNADLFPAGFCSPTK

Nucleotide


Download         Length: 444 bp        

>NTDB_id=492873 INQ00_RS00250 WP_197546948.1 50614..51057(+) (pilA) [Haemophilus parainfluenzae strain M1C160_1]
ATGAAACTGACTTTTTCTAAACCCTTACATAAAGGTTTTACGTTAATTGAATTGATGATCGTGATTGCGATTATTGCTAT
TCTCGCGACGATCGCCATTCCGTCTTATCAAAATTATACCAAAAAAGCAGCCATCTCCGAATTATTGCAAGCTTCTGCAC
CTTATAAATCTGATGTGGAATTATGTGTCTATAGCACCAATGCCCCAACAAACTGTTCTGGTGGTTCAAATGGCATTACA
GCAGACATTACTACTGCAAAAGGCTATGTTAAATCCATTACCACGAAATCGGGTGTGATTACGGTAACAGGAAATGGCGC
ATTGGATGGGATCAGTTATTCTTTAACGGCGACAGGCTCAACCTCATCAGGTGTGACCTGGACAACCGCTTGCCCGAGCA
ATGCGGATTTATTCCCTGCGGGCTTCTGCTCTCCTACCAAATAG

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(10-32)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

75

97.959

0.735
  pilA Haemophilus influenzae Rd KW20

72.917

97.959

0.714
  pilA Glaesserella parasuis strain SC1401

56.081

100

0.565
  pilA Vibrio campbellii strain DS40M4

41.096

99.32

0.408
  pilE Neisseria gonorrhoeae MS11

41.221

89.116

0.367