Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   IMF22_RS21850 Genome accession   NZ_CP063073
Coordinates   4888634..4889959 (-) Length   441 a.a.
NCBI ID   WP_197629983.1    Uniprot ID   A0A7M1KT37
Organism   Pseudomonas poae strain PMA22     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4883634..4894959
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IMF22_RS21835 (IMF22_21835) - 4885313..4886875 (-) 1563 WP_197625861.1 SPOR domain-containing protein -
  IMF22_RS21840 (IMF22_21840) aroB 4886886..4887986 (-) 1101 WP_177017297.1 3-dehydroquinate synthase -
  IMF22_RS21845 (IMF22_21845) aroK 4888111..4888629 (-) 519 WP_017138567.1 shikimate kinase AroK -
  IMF22_RS21850 (IMF22_21850) pilQ 4888634..4889959 (-) 1326 WP_197629983.1 type IV pilus secretin PilQ Machinery gene
  IMF22_RS21855 (IMF22_21855) - 4890029..4891000 (-) 972 WP_197625863.1 pilus assembly protein PilP -
  IMF22_RS21860 (IMF22_21860) - 4890997..4891548 (-) 552 WP_197625865.1 PilN domain-containing protein -
  IMF22_RS21865 (IMF22_21865) pilM 4891545..4892201 (-) 657 WP_197629984.1 type IV pilus assembly protein PilM -
  IMF22_RS21870 (IMF22_21870) - 4892431..4894893 (+) 2463 WP_371317686.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 441 a.a.        Molecular weight: 47941.06 Da        Isoelectric Point: 4.9400

>NTDB_id=492738 IMF22_RS21850 WP_197629983.1 4888634..4889959(-) (pilQ) [Pseudomonas poae strain PMA22]
MALAVPNRVDLIQLPPPGRAVPSTYSGDKLNLNFQNIELRAVLQQIADVAGLNLVTSDEVQGSITLRLKDVPWDQALDLV
LQTKGLDKRVKAGVLLVAPAEELAARELLVLESNKQMADLAPLRRELLQVNYAKAADLAKLFQSVTTLDGLTDERGSVAV
DDRTNNIIAYQTGERLEELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGRLNRGNWGAGGIGKPLAEGVEPPA
SPSSSPFVDLGSLTGTAGLGIAYITDNLLLDLELTAMEKTGNGEIVSQPKVVTSDKETARILKGTEIPYQEATGRGATSV
SFKEASLSLEVTPQITPDGWVIMEVKVTKDEPDYLNKLNDVPPIKKNEVNAKVLVKDGETIVIGGVFSNTQSKVVDKVPF
LGDVPYLGRLFRRDVVAQRKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 1326 bp        

>NTDB_id=492738 IMF22_RS21850 WP_197629983.1 4888634..4889959(-) (pilQ) [Pseudomonas poae strain PMA22]
ATGGCCCTTGCTGTGCCCAACCGTGTAGATCTGATCCAACTGCCGCCGCCGGGTCGCGCGGTGCCAAGCACCTATAGCGG
TGACAAGCTGAACCTCAACTTCCAGAACATCGAGTTGCGTGCGGTATTGCAGCAGATAGCGGATGTGGCCGGGCTCAACC
TGGTCACCAGCGACGAGGTGCAAGGCTCGATCACCCTGCGACTCAAGGATGTGCCCTGGGATCAGGCGCTGGACCTGGTG
TTGCAAACCAAAGGGTTGGACAAGCGCGTGAAAGCCGGCGTTTTGCTGGTGGCGCCCGCCGAAGAATTGGCCGCCCGTGA
GTTGCTGGTGTTGGAGTCGAACAAGCAGATGGCCGACTTGGCGCCATTGCGCCGGGAGCTGTTACAGGTCAATTACGCCA
AGGCCGCGGACCTGGCCAAGCTGTTTCAGTCGGTGACGACCCTGGATGGCCTCACCGACGAGCGCGGTTCAGTGGCGGTG
GATGACCGCACCAACAATATCATTGCCTACCAGACCGGCGAGCGGCTCGAAGAGCTGCGACGGATCGTGGCGCAGTTGGA
TATTCCAGTGCGCCAAGTAATGATTGAGGCGCGCATCGTCGAGGCTAATGTCGATTACGACAAAAGCCTGGGCGTGCGCT
GGGGCGGGCGCCTTAATCGTGGCAATTGGGGCGCGGGCGGCATCGGCAAGCCGTTGGCTGAGGGCGTTGAACCACCGGCG
AGCCCGTCCAGCTCGCCGTTTGTTGACTTGGGCTCACTCACCGGCACTGCGGGTTTGGGCATTGCCTATATCACCGACAA
CCTGTTGTTGGACCTGGAACTGACGGCCATGGAGAAAACCGGCAACGGCGAAATCGTCTCGCAGCCCAAGGTCGTCACCT
CCGACAAGGAGACCGCGCGCATCCTCAAAGGCACCGAGATTCCCTATCAGGAAGCCACCGGCAGAGGCGCCACGTCGGTG
TCCTTCAAGGAGGCCTCGCTGTCGTTGGAGGTGACCCCGCAAATCACGCCCGATGGCTGGGTGATCATGGAGGTCAAGGT
CACCAAGGATGAGCCCGACTACCTGAACAAACTCAACGACGTGCCGCCGATCAAGAAAAACGAAGTCAACGCCAAGGTGC
TGGTCAAGGACGGCGAGACCATCGTCATCGGCGGGGTTTTCTCCAATACCCAAAGCAAAGTGGTAGATAAAGTGCCATTT
TTGGGCGATGTGCCGTATCTTGGCCGCCTTTTCCGGCGCGATGTTGTGGCGCAAAGAAAATCCGAGCTGCTGGTATTCCT
GACTCCGCGTATTATGAATAACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(127-192)

Bacterial type II and III secretion system protein (Secretin)

(276-432)

Secretin and TonB N terminus short domain (STN)

(54-99)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7M1KT37

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

71.33

98.866

0.705
  pilQ Acinetobacter baumannii strain A118

40.568

100

0.454
  pilQ Acinetobacter baumannii D1279779

40.568

100

0.454
  comQ Acinetobacter baylyi ADP1

42.667

100

0.435
  pilQ Vibrio campbellii strain DS40M4

43.972

95.918

0.422
  pilQ Legionella pneumophila strain ERS1305867

44.286

95.238

0.422
  pilQ Legionella pneumophila strain Lp02

44.076

95.692

0.422
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

42.686

94.558

0.404
  pilQ Vibrio cholerae strain A1552

42.686

94.558

0.404
  pilQ Neisseria gonorrhoeae strain FA1090

36.301

99.32

0.361