Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   IHV77_RS07860 Genome accession   NZ_CP063056
Coordinates   1659278..1659715 (-) Length   145 a.a.
NCBI ID   WP_194811433.1    Uniprot ID   -
Organism   Rodentibacter haemolyticus strain DSM 111151     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1654278..1664715
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IHV77_RS07830 (IHV77_07830) - 1654880..1655161 (-) 282 WP_194811428.1 GNAT family N-acetyltransferase -
  IHV77_RS07835 (IHV77_07835) yacG 1655158..1655364 (-) 207 WP_194811429.1 DNA gyrase inhibitor YacG -
  IHV77_RS07840 (IHV77_07840) coaE 1655357..1655977 (-) 621 WP_194813256.1 dephospho-CoA kinase -
  IHV77_RS07845 (IHV77_07845) pilD 1655997..1656686 (-) 690 WP_194811430.1 A24 family peptidase Machinery gene
  IHV77_RS07850 (IHV77_07850) pilC 1656683..1657903 (-) 1221 WP_194811431.1 type II secretion system F family protein Machinery gene
  IHV77_RS07855 (IHV77_07855) pilB 1657905..1659281 (-) 1377 WP_194811432.1 GspE/PulE family protein Machinery gene
  IHV77_RS07860 (IHV77_07860) pilA 1659278..1659715 (-) 438 WP_194811433.1 prepilin peptidase-dependent pilin Machinery gene
  IHV77_RS07865 (IHV77_07865) ampD 1659830..1660393 (+) 564 WP_194811434.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  IHV77_RS07870 (IHV77_07870) rppH 1660911..1661379 (+) 469 Protein_1520 RNA pyrophosphohydrolase -
  IHV77_RS07875 (IHV77_07875) lgt 1661624..1662430 (+) 807 WP_408635263.1 prolipoprotein diacylglyceryl transferase -
  IHV77_RS07880 (IHV77_07880) - 1662441..1663292 (+) 852 WP_194811436.1 thymidylate synthase -
  IHV77_RS07885 (IHV77_07885) tadA 1663331..1663801 (+) 471 WP_194811437.1 tRNA adenosine(34) deaminase TadA -
  IHV77_RS07890 (IHV77_07890) ung 1663820..1664479 (-) 660 WP_194811438.1 uracil-DNA glycosylase -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 14953.18 Da        Isoelectric Point: 8.4749

>NTDB_id=492611 IHV77_RS07860 WP_194811433.1 1659278..1659715(-) (pilA) [Rodentibacter haemolyticus strain DSM 111151]
MKLTSQRTLKKGFTLIELMIVIAIIAILATIAIPSYQNYTKKAAMSELLQAAAPFKSDVELCIYGTGTTANCSGGSNGIA
ADITSAKGYVKSISTNAGVITVVGNGTLDGVQYTLTATGDNARGVSWSATCNGDASLFPAGFCTR

Nucleotide


Download         Length: 438 bp        

>NTDB_id=492611 IHV77_RS07860 WP_194811433.1 1659278..1659715(-) (pilA) [Rodentibacter haemolyticus strain DSM 111151]
ATGAAACTCACCTCTCAACGCACATTGAAAAAAGGATTTACGCTAATTGAATTAATGATTGTGATCGCCATCATCGCGAT
TCTCGCGACAATTGCGATTCCCTCTTACCAGAATTATACAAAAAAGGCGGCGATGTCTGAACTGTTACAAGCCGCAGCCC
CTTTTAAATCCGACGTAGAACTCTGCATTTACGGCACGGGAACAACCGCAAATTGTAGTGGCGGCTCAAACGGTATTGCT
GCCGATATTACCTCAGCAAAAGGCTATGTGAAAAGTATTAGCACTAACGCCGGTGTAATTACCGTTGTTGGCAACGGCAC
GTTAGACGGAGTGCAATATACCTTAACCGCAACCGGTGATAATGCTAGAGGAGTAAGCTGGTCTGCAACCTGTAACGGTG
ATGCTTCCTTATTTCCGGCAGGGTTCTGTACAAGATGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(10-32)

Pilin (bacterial filament) (Pilin)

(42-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

75

99.31

0.745
  pilA Haemophilus influenzae Rd KW20

72.222

99.31

0.717
  pilA Glaesserella parasuis strain SC1401

59.441

98.621

0.586
  pilA Vibrio campbellii strain DS40M4

37.419

100

0.4
  comP Acinetobacter baylyi ADP1

40.426

97.241

0.393
  pilA2 Legionella pneumophila strain ERS1305867

41.985

90.345

0.379
  pilA2 Legionella pneumophila str. Paris

41.985

90.345

0.379
  pilA/pilAI Pseudomonas stutzeri DSM 10701

38.732

97.931

0.379