Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   IL989_RS12030 Genome accession   NZ_CP062984
Coordinates   2462391..2463005 (-) Length   204 a.a.
NCBI ID   WP_193350455.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain PP19 isolate PP19     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2457391..2468005
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IL989_RS12000 (IL989_12000) rpsT 2457691..2457957 (+) 267 WP_003152876.1 30S ribosomal protein S20 -
  IL989_RS12005 (IL989_12005) holA 2457975..2459016 (-) 1042 Protein_2334 DNA polymerase III subunit delta -
  IL989_RS12010 (IL989_12010) - 2459056..2459210 (-) 155 Protein_2335 hypothetical protein -
  IL989_RS12015 (IL989_12015) - 2459251..2459385 (+) 135 WP_003152870.1 YqzM family protein -
  IL989_RS12020 (IL989_12020) comEC 2459404..2461755 (-) 2352 WP_193350453.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  IL989_RS12025 (IL989_12025) - 2461756..2462325 (-) 570 WP_193350454.1 ComE operon protein 2 -
  IL989_RS12030 (IL989_12030) comEA 2462391..2463005 (-) 615 WP_193350455.1 helix-hairpin-helix domain-containing protein Machinery gene
  IL989_RS12035 (IL989_12035) comER 2463064..2463885 (+) 822 WP_045510457.1 late competence protein ComER -
  IL989_RS12040 (IL989_12040) - 2463954..2464691 (-) 738 WP_014470703.1 class I SAM-dependent methyltransferase -
  IL989_RS12045 (IL989_12045) rsfS 2464688..2465044 (-) 357 WP_045510454.1 ribosome silencing factor -
  IL989_RS12050 (IL989_12050) yqeK 2465061..2465621 (-) 561 WP_193350456.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  IL989_RS12055 (IL989_12055) - 2465611..2466180 (-) 570 WP_065522728.1 nicotinate-nucleotide adenylyltransferase -
  IL989_RS12060 (IL989_12060) yhbY 2466191..2466481 (-) 291 WP_014470707.1 ribosome assembly RNA-binding protein YhbY -
  IL989_RS12065 (IL989_12065) aroE 2466475..2467317 (-) 843 WP_014470708.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 204 a.a.        Molecular weight: 22164.40 Da        Isoelectric Point: 5.4688

>NTDB_id=492263 IL989_RS12030 WP_193350455.1 2462391..2463005(-) (comEA) [Bacillus amyloliquefaciens strain PP19 isolate PP19]
MTKVMMTERQRQYLKILGVCLAAGAVLICILLFRPEKEEPVKQQSVLGQTASAAVTDKKDTADDKIMIDIKGAVRHPGVY
EMKAGDRVTQAIKKAGGLKEKADELNVNLAEQLQDGTIVYIPSEGEEANPPKTAAGEKENASVNINTASLDELQAIPGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide


Download         Length: 615 bp        

>NTDB_id=492263 IL989_RS12030 WP_193350455.1 2462391..2463005(-) (comEA) [Bacillus amyloliquefaciens strain PP19 isolate PP19]
ATGACGAAAGTGATGATGACGGAGCGGCAGAGGCAGTACCTGAAAATTCTTGGCGTTTGTCTTGCGGCAGGGGCGGTTTT
GATCTGCATTTTGCTGTTTCGTCCCGAAAAAGAAGAGCCTGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAG
CCGTCACGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATATTAAAGGAGCGGTCCGACATCCCGGCGTGTAT
GAAATGAAGGCGGGAGACAGAGTGACGCAAGCCATTAAGAAAGCCGGCGGTCTGAAGGAAAAGGCGGATGAACTGAATGT
TAATTTAGCAGAGCAGTTGCAGGACGGGACGATCGTTTATATCCCGAGTGAAGGCGAAGAGGCGAATCCCCCGAAAACCG
CAGCAGGTGAAAAGGAGAATGCTTCTGTCAATATCAATACGGCATCTCTTGACGAACTTCAGGCGATTCCCGGTGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAATGGGCGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGTTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(141-202)

SLBB domain (SLBB)

(68-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

57.436

95.588

0.549
  comEA/celA/cilE Streptococcus pneumoniae R6

37.788

100

0.402
  comEA/celA/cilE Streptococcus pneumoniae Rx1

37.788

100

0.402
  comEA/celA/cilE Streptococcus pneumoniae D39

37.788

100

0.402
  comEA/celA/cilE Streptococcus mitis NCTC 12261

37.327

100

0.397
  comEA/celA/cilE Streptococcus pneumoniae TIGR4

37.674

100

0.397
  comEA Staphylococcus aureus MW2

35.294

100

0.382
  comEA Lactococcus lactis subsp. cremoris KW2

34.821

100

0.382
  comEA Staphylococcus aureus N315

34.842

100

0.377
  comEA/celA/cilE Streptococcus mitis SK321

35.814

100

0.377
  comEA Latilactobacillus sakei subsp. sakei 23K

32.051

100

0.368